Optical tweezers in single-molecule biophysics [PDF]
Nature Reviews Methods Primers, 2021 Optical tweezers have become the method of choice in single-molecule manipulation studies. In this Primer, we first review the physical principles of optical tweezers and the characteristics that make them a powerful tool to investigate single molecules.
Michelle D Wang +2 more
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Single-molecule biophysics: at the interface of biology, physics and chemistry [PDF]
Journal of the Royal Society Interface, 2008 Single-molecule methods have matured into powerful and popular tools to probe the complex behaviour of biological molecules, due to their unique abilities to probe molecular structure, dynamics and function, unhindered by the averaging inherent in ensemble experiments.
Edward Lemke +2 more
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Multiple events on single molecules: Unbiased estimation in single-molecule biophysics [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2006 Most analyses of single-molecule experiments consist of binning experimental outcomes into a histogram and finding the parameters that optimize the fit of this histogram to a given data model. Here we show that such an approach can introduce biases in the estimation of the parameters, thus great care must be taken in the estimation of model parameters ...
Nynke Dekker +2 more
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Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics. [PDF]
Int J Mol Sci, 2022 Cellular functions depend on the dynamic assembly of protein regulator complexes at specific cellular locations. Single Molecule Tracking (SMT) is a method of choice for the biochemical characterization of protein dynamics in vitro and in vivo. SMT follows individual molecules in live cells and provides direct information about their behavior.
Ball DA, Jalloh B, Karpova TS.
europepmc +3 more sources
Statistics and Related Topics in Single-Molecule Biophysics. [PDF]
Annu Rev Stat Appl, 2014 Since the universal acceptance of atoms and molecules as the fundamental constituents of matter in the early-twentieth century, molecular physics, chemistry, and molecular biology have all experienced major theoretical breakthroughs. Although researchers had to wait until the 1970s to see individual biological macromolecules one at a time in action ...
Qian H, Kou SC.
europepmc +4 more sources
Graphene Energy Transfer for Single‐Molecule Biophysics, Biosensing, and Super‐Resolution Microscopy [PDF]
Advanced Materials, 2021 Johann Bohlen +2 more
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Japan–US symposium on motor proteins and associated single-molecule biophysics [PDF]
Biophysics and Physicobiology, 2022 Kumiko Hayashi, Jakia Jannat Keya
doaj +2 more sources
The third Japan-U.S. symposium on motor proteins and associated single-molecule biophysics [PDF]
Biophysics and Physicobiology, 2023 Tomohiro Shima, Kumiko Hayashi
doaj +2 more sources
Towards physiological complexity with in vitro single-molecule biophysics [PDF]
Philosophical Transactions of the Royal Society B: Biological Sciences, 2013 Eric Greene, Daniel Duzdevich
exaly +2 more sources
Photophysics of Fluorescent Probes for Single-Molecule Biophysics and Super-Resolution Imaging [PDF]
Annual Review of Physical Chemistry, 2012 Jingyi Fei, Philip Tinnefeld
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