Results 1 to 10 of about 479,986 (210)
Ultrasensitive Detection of Attomolar Protein Concentrations by Dropcast Single Molecule Assays. [PDF]
J Am Chem Soc, 2020 Measurements of very low levels of biomolecules, including proteins and nucleic acids, remain a critical challenge in many clinical diagnostic applications due to insufficient sensitivity. While digital measurement methods such as Single Molecule Arrays (Simoa), or digital ELISA, have made significant advances in sensitivity, there are still many ...
Wu C, Garden PM, Walt DR.
europepmc +5 more sources
Influence of fluorescent tag on the motility properties of kinesin-1 in single-molecule assays. [PDF]
Biophys J, 2015 Molecular motors such as kinesin and dynein use the energy derived from ATP hydrolysis to walk processively along microtubule tracks and transport various cargoes inside the cell.
Norris SR, Núñez MF, Verhey KJ.
europepmc +2 more sources
Advances in Optical Single‐Molecule Detection: En Route to Supersensitive Bioaffinity Assays
Angewandte Chemie - International Edition, 2020 The ability to detect low concentrations of analytes and in particular low-abundance biomarkers is of fundamental importance, e.g., for early-stage disease diagnosis.
Zdenek Farka +2 more
exaly +2 more sources
Chemistry - A European Journal, 2022 Single‐molecule assays often require functionalized surfaces. One approach for microtubule assays renders surfaces hydrophobic and uses amphiphilic blocking agents. However, the optimal hydrophobicity is unclear, protocols take long, produce toxic waste,
Heiko Peisert, Erik Schaffer
exaly +2 more sources
How Nanoparticles Transform Single Molecule Measurements into Quantitative Sensors
Advanced Materials, 2020 Single molecule measurements are revolutionizing the understanding of the stochastics of behavior of single molecules. There is a common theme referred to as a near-field approach, in how many single molecule measurements are being performed in assays ...
Yanfang Wu +2 more
exaly +3 more sources
Protocol for single-molecule pull-down of fluorescently tagged oligomers from cell lysates
STAR Protocols, 2023 Summary: Mutations in intrinsically disordered proteins drive the irreversible formation of pathological aggregates, a hallmark of neurodegenerative diseases.
Nathalie Djaja, Sua Myong
doaj +1 more source
Quantifying transport dynamics with three-dimensional single-particle tracking in adherent cells
STAR Protocols, 2022 Summary: Intracellular transport plays an important role in maintaining the physiological functions of cells. Here, we describe a protocol for 3D single-particle tracking within living cells.
Chao Jiang +3 more
doaj +1 more source
Protocol for generation and regeneration of PEG-passivated slides for single-molecule measurements
STAR Protocols, 2022 Summary: Single-molecule fluorescence detection by total internal reflection microscope requires surface passivation by polyethylene glycol (PEG) coating, which is labor intensive and is only good for one or two experiments. Here, we present an efficient
Tapas Paul, Sua Myong
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Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis
STAR Protocols, 2021 Summary: Here, we describe a rapid and versatile protocol to generate gapped DNA substrates for single-molecule (SM) analysis using optical tweezers via site-specific Cas9 nicking and force-induced melting. We provide examples of single-stranded (ss) DNA
Ondrej Belan +6 more
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STAR Protocols, 2022 Summary: Aquaporin water channels (AQPs) are membrane proteins that maintain cellular water homeostasis. The interactions between human AQPs and other proteins play crucial roles in AQP regulation by both gating and trafficking.
Tamim Al-Jubair +7 more
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