Results 1 to 10 of about 324,481 (117)
Highly sensitive volumetric single-molecule imaging [PDF]
NanophotonicsVolumetric subcellular imaging has long been essential for studying structures and dynamics in cells and tissues. However, due to limited imaging speed and depth of field, it has been challenging to perform live-cell imaging and single-particle tracking.
Wang Le-Mei, Kim Jiah, Han Kyu Young
doaj +2 more sources
Automated single-molecule imaging in living cells
Nature Communications, 2018 Large scale live cell screens often lack single-molecule resolution. Here the authors present an artificial intelligence-assisted TIRF microscope with automated cell searching and focusing, and use it for high-throughput single-molecule imaging of EGFR ...
Masato Yasui +4 more
doaj +2 more sources
Spontaneous Confinement of mRNA Molecules at Biomolecular Condensate Boundaries
Cells, 2023 Cellular biomolecular condensates, termed ribonucleoprotein (RNP) granules, are often enriched in messenger RNA (mRNA) molecules relative to the surrounding cytoplasm.
Rebecca T. Perelman +3 more
doaj +1 more source
Biomechanics of T Cell Dysfunctions in Chronic Diseases
Frontiers in Immunology, 2021 Understanding the mechanisms behind T cell dysfunctions during chronic diseases is critical in developing effective immunotherapies. As demonstrated by several animal models and human studies, T cell dysfunctions are induced during chronic diseases ...
Sachith D. Gunasinghe +7 more
doaj +1 more source
Frontiers in Bioinformatics, 2021 Single molecule localisation microscopy (SMLM) is a powerful tool that has revealed the spatial arrangement of cell surface signalling proteins, producing data of enormous complexity.
Kristen Feher +12 more
doaj +1 more source
Duplex DNA engagement and RPA oppositely regulate the DNA-unwinding rate of CMG helicase
Nature Communications, 2020 During eukaryotic chromosome replication cells utilize ring-shaped CMG helicase that separates the two strands of the DNA double helix. Here the authors reveal that CMG helicase activity is inhibited by duplex DNA engagement at the fork, which is ...
Hazal B. Kose +4 more
doaj +1 more source
Live cell imaging of single RNA molecules with fluorogenic Mango II arrays
Nature Communications, 2020 Fluorogenic RNA aptamers have been used for RNA imaging, but folding and fluorescence stability often limited their use in high resolution applications.
Adam D. Cawte +2 more
doaj +1 more source
Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis
STAR Protocols, 2021 Summary: Here, we describe a rapid and versatile protocol to generate gapped DNA substrates for single-molecule (SM) analysis using optical tweezers via site-specific Cas9 nicking and force-induced melting. We provide examples of single-stranded (ss) DNA
Ondrej Belan +6 more
doaj +1 more source
Bio-Protocol, 2022 The human immunodeficiency virus 1 (HIV-1) consists of a viral membrane surrounding the conical capsid. The capsid is a protein container assembled from approximately 1,500 copies of the viral capsid protein (CA), functioning as a reaction and transport ...
Derrick Lau +3 more
doaj +1 more source
Genetic screening for anticancer genes highlights FBLN5 as a synthetic lethal partner of MYC
Cell Communication and Signaling, 2023 Background When ectopically overexpressed, anticancer genes, such as TRAIL, PAR4 and ORCTL3, specifically destroy tumour cells without harming untransformed cells. Anticancer genes can not only serve as powerful tumour specific therapy tools but studying
Motasim Masood +6 more
doaj +1 more source