Results 1 to 10 of about 310,324 (162)
Highly sensitive volumetric single-molecule imaging [PDF]
NanophotonicsVolumetric subcellular imaging has long been essential for studying structures and dynamics in cells and tissues. However, due to limited imaging speed and depth of field, it has been challenging to perform live-cell imaging and single-particle tracking.
Wang Le-Mei, Kim Jiah, Han Kyu Young
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DNA Manipulation and Single-Molecule Imaging [PDF]
Molecules, 2021 DNA replication, repair, and recombination in the cell play a significant role in the regulation of the inheritance, maintenance, and transfer of genetic information.
Shunsuke Takahashi +2 more
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Single-molecule imaging in the primary cilium. [PDF]
Methods Cell Biol, 2023 The primary cilium is an important signaling organelle critical for normal development and tissue homeostasis. Its small dimensions and complexity necessitate advanced imaging approaches to uncover the molecular mechanisms behind its function. Here, we outline how single-molecule fluorescence microscopy can be used for tracking molecular dynamics and ...
Weiss LE +8 more
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Single molecule imaging simulations with advanced fluorophore photophysics [PDF]
Communications Biology, 2023 Single Molecule Imaging Simulator (SMIS) simulates how the photophysical behavior of fluorophores in single-molecule localization experiments influences experimental data, as evidenced by applying SMIS to several imaging scenarios.
Dominique Bourgeois
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Single Molecule Imaging and Manipulation
CHIMIA, 2002 The atomic force microscope (AFM) and optical tweezers are tools that allow single biomolecules to be imaged and manipulated. Progress in instrumentation, sample preparation, and image acquisition conditions make novel applications of these ...
Martin Hegner, Andreas Engel
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Automated single-molecule imaging in living cells
Nature Communications, 2018 Large scale live cell screens often lack single-molecule resolution. Here the authors present an artificial intelligence-assisted TIRF microscope with automated cell searching and focusing, and use it for high-throughput single-molecule imaging of EGFR ...
Masato Yasui +4 more
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Spontaneous Confinement of mRNA Molecules at Biomolecular Condensate Boundaries
Cells, 2023 Cellular biomolecular condensates, termed ribonucleoprotein (RNP) granules, are often enriched in messenger RNA (mRNA) molecules relative to the surrounding cytoplasm.
Rebecca T. Perelman +3 more
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Imaging of single molecule diffusion. [PDF]
Proceedings of the National Academy of Sciences, 1996 In recent years observations at the level of individual atoms and molecules became possible by microscopy and spectroscopy. Imaging of single fluorescence molecules has been achieved but has so far been restricted to molecules in the immobile state. Here we provide methodology for visualization of the motion of individual fluorescent molecules.
Schmidt, T. +4 more
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Biomechanics of T Cell Dysfunctions in Chronic Diseases
Frontiers in Immunology, 2021 Understanding the mechanisms behind T cell dysfunctions during chronic diseases is critical in developing effective immunotherapies. As demonstrated by several animal models and human studies, T cell dysfunctions are induced during chronic diseases ...
Sachith D. Gunasinghe +7 more
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Imaging of Tautomerism in a Single Molecule [PDF]
Journal of the American Chemical Society, 2005 Fluorescence imaging is used to visualize directly the transfer of two inner hydrogen atoms in single porphycene molecules. This reaction leads to a chemically equivalent but differently oriented structure and hence results in a rotation of the transition dipole moments.
Piwoński, Hubert +5 more
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