Results 11 to 20 of about 7,224,188 (257)

Trends in Single-Molecule Total Internal Reflection Fluorescence Imaging and Their Biological Applications with Lab-on-a-Chip Technology

open access: yesSensors, 2023
Single-molecule imaging technologies, especially those based on fluorescence, have been developed to probe both the equilibrium and dynamic properties of biomolecules at the single-molecular and quantitative levels. In this review, we provide an overview
Louis Colson   +6 more
doaj   +2 more sources

Single-molecule nano-optics at low temperature [PDF]

open access: yes, 2006
The work presented in this thesis involves experiments on single molecules at low temperature. At low temperature, many disturbing temperature activated processes are frozen out, resulting in extremely sharp zero-phonon lines in the fluorescence ex ...
Butter, Jacqueline Yvonne Paula
core   +1 more source

songuno1/single-molecule-assay: Single-molecule assay analysis

open access: yes, 2023
Single-molecule assay analysis tool for "Transcriptional pause extension benefits the stand-by rather than catch-up Rho-dependent termination"
Eunho Song
core   +1 more source

SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling

open access: yeseLife, 2022
T cell activation requires engagement of a cognate antigen by the T cell receptor (TCR) and the co-stimulatory signal of CD28. Both TCR and CD28 aggregate into clusters at the plasma membrane of activated T cells.
Manuela Ecker   +9 more
doaj   +1 more source

Real-Time analysis and visualization for single-molecule based super-resolution microscopy [PDF]

open access: yes, 2013
Accurate multidimensional localization of isolated fluorescent emitters is a time consuming process in single-molecule based super-resolution microscopy.
Jean-Baptiste Sibarita   +9 more
core   +1 more source

Single molecule detection for in vitro diagnostics

open access: yes, 2022
Paper 68620HIn this paper we present a novel highly sensitive detection system for diagnostic applications. The system is designed to meet the needs of medical diagnostics for reliable measurements of pathogens and biomarkers in the low concentration ...
Greiner, B.   +7 more
core   +1 more source

T Cell Membrane Heterogeneity Aids Antigen Recognition and T Cell Activation

open access: yesFrontiers in Cell and Developmental Biology, 2020
T cells are critical for co-ordinating the immune response. T cells are activated when their surface T cell receptors (TCRs) engage cognate antigens in the form of peptide-major histocompatibility complexes (pMHC) presented on the surface of antigen ...
Megan V. Farrell   +7 more
doaj   +1 more source

Building a Total Internal Reflection Microscope (TIRF) with Active Stabilization (Feedback SMLM)

open access: yesBio-Protocol, 2021
The data quality of high-resolution imaging can be markedly improved with active stabilization, which is based on feedback loops within the microscope that maintain the sample in the same location throughout the experiment.
Simao Coelho   +3 more
doaj   +1 more source

Fluorescence Microscopy Assay to Measure HIV-1 Capsid Uncoating Kinetics in vitro

open access: yesBio-Protocol, 2019
The stability of the HIV-1 capsid and the spatiotemporal control of its disassembly, a process called uncoating, need to be finely tuned for infection to proceed.
Chantal Márquez   +6 more
doaj   +1 more source

Rapid whole cell imaging reveals a calcium-APPL1-dynein nexus that regulates cohort trafficking of stimulated EGF receptors

open access: yesCommunications Biology, 2021
York et al employ lattice light-sheet microscopy to study the initial events following activation of the epidermal growth factor receptor (EGFR) in whole live cells.
H. M. York   +9 more
doaj   +1 more source

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