Results 11 to 20 of about 310,423 (261)
Single-Molecule Anisotropy Imaging [PDF]
Biophysical Journal, 1999 A novel method, single-molecule anisotropy imaging, has been employed to simultaneously study lateral and rotational diffusion of fluorescence-labeled lipids on supported phospholipid membranes. In a fluid membrane composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, in which the rotational diffusion time is on the order of the excited-state ...
Harms, G.S. +4 more
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Imaging proteins at the single-molecule level [PDF]
Proceedings of the National Academy of Sciences, 2017 Significance We report a method to image and reveal structural details of proteins on a truly single-molecule level. Low-energy electron holography is used to image individual proteins electrospray deposited on freestanding graphene.
Jean-Nicolas Longchamp +6 more
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Frontiers in Bioinformatics, 2021 Single molecule localisation microscopy (SMLM) is a powerful tool that has revealed the spatial arrangement of cell surface signalling proteins, producing data of enormous complexity.
Kristen Feher +12 more
doaj +1 more source
Imaging single glycan molecules [PDF]
Nature Methods, 2020 Direct imaging of carbohydrates using electrospray ion beam deposition and scanning tunneling microscopy.
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Duplex DNA engagement and RPA oppositely regulate the DNA-unwinding rate of CMG helicase
Nature Communications, 2020 During eukaryotic chromosome replication cells utilize ring-shaped CMG helicase that separates the two strands of the DNA double helix. Here the authors reveal that CMG helicase activity is inhibited by duplex DNA engagement at the fork, which is ...
Hazal B. Kose +4 more
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Live cell imaging of single RNA molecules with fluorogenic Mango II arrays
Nature Communications, 2020 Fluorogenic RNA aptamers have been used for RNA imaging, but folding and fluorescence stability often limited their use in high resolution applications.
Adam D. Cawte +2 more
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Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis
STAR Protocols, 2021 Summary: Here, we describe a rapid and versatile protocol to generate gapped DNA substrates for single-molecule (SM) analysis using optical tweezers via site-specific Cas9 nicking and force-induced melting. We provide examples of single-stranded (ss) DNA
Ondrej Belan +6 more
doaj +1 more source
Bio-Protocol, 2022 The human immunodeficiency virus 1 (HIV-1) consists of a viral membrane surrounding the conical capsid. The capsid is a protein container assembled from approximately 1,500 copies of the viral capsid protein (CA), functioning as a reaction and transport ...
Derrick Lau +3 more
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Genetic screening for anticancer genes highlights FBLN5 as a synthetic lethal partner of MYC
Cell Communication and Signaling, 2023 Background When ectopically overexpressed, anticancer genes, such as TRAIL, PAR4 and ORCTL3, specifically destroy tumour cells without harming untransformed cells. Anticancer genes can not only serve as powerful tumour specific therapy tools but studying
Motasim Masood +6 more
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SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling
eLife, 2022 T cell activation requires engagement of a cognate antigen by the T cell receptor (TCR) and the co-stimulatory signal of CD28. Both TCR and CD28 aggregate into clusters at the plasma membrane of activated T cells.
Manuela Ecker +9 more
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