Results 21 to 30 of about 310,423 (261)
Verification of Single-Molecule Imaging and Single-Molecule Measurements
Journal of Robotics and Mechatronics, 2010 Single-molecule imaging and single-molecule measurements constitute an integral part of life science researches. The research method, which allows direct measurements of individual motions and events of biomolecules, has contributed much to the development of life science research by providing us with numerous findings. It is left up to the judgment of
Akihiko Ishijima +2 more
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Single molecule imaging of mRNA splicing [PDF]
Nucleic Acids Symposium Series, 2005 Most eukaryotic mRNA precursors (pre-mRNAs) are interrupted by non-coding intervening sequences known as introns which must be precisely and efficiently removed to yield correct mature mRNAs. The process of intron excision, pre-mRNA splicing, takes place in a multicomponent ribonucleoprotein complexes which assemble in a stepwise pathway.
Yo, Ishihama +3 more
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T Cell Membrane Heterogeneity Aids Antigen Recognition and T Cell Activation
Frontiers in Cell and Developmental Biology, 2020 T cells are critical for co-ordinating the immune response. T cells are activated when their surface T cell receptors (TCRs) engage cognate antigens in the form of peptide-major histocompatibility complexes (pMHC) presented on the surface of antigen ...
Megan V. Farrell +7 more
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Building a Total Internal Reflection Microscope (TIRF) with Active Stabilization (Feedback SMLM)
Bio-Protocol, 2021 The data quality of high-resolution imaging can be markedly improved with active stabilization, which is based on feedback loops within the microscope that maintain the sample in the same location throughout the experiment.
Simao Coelho +3 more
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Fluorescence Microscopy Assay to Measure HIV-1 Capsid Uncoating Kinetics in vitro
Bio-Protocol, 2019 The stability of the HIV-1 capsid and the spatiotemporal control of its disassembly, a process called uncoating, need to be finely tuned for infection to proceed.
Chantal Márquez +6 more
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Communications Biology, 2021 York et al employ lattice light-sheet microscopy to study the initial events following activation of the epidermal growth factor receptor (EGFR) in whole live cells.
H. M. York +9 more
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Functional analysis of the secondary HIV-1 capsid binding site in the host protein cyclophilin A
Retrovirology, 2019 Background Efficient HIV-1 replication depends on interaction of the viral capsid with the host protein cyclophilin A (CypA). CypA, a peptidylprolyl isomerase, binds to an exposed loop in the viral CA protein via the enzyme’s active site.
Wang Peng +9 more
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Serotonin: an overlooked regulator of endocytosis and endosomal sorting?
Biology Open, 2022 Serotonin is a neurotransmitter and a hormone that is typically associated with regulating our mood. However, the serotonin transporter and receptors are expressed throughout the body, highlighting the much broader, systemic role of serotonin in ...
Gregory Redpath, Nikita Deo
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Nature Communications, 2018 Endocytosis of T cell receptors (TCR) and their polarized recycling back to the plasma membrane is crucial for T cell activation; however, the underlying mechanisms remain unclear.
Ewoud B. Compeer +15 more
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Kinetics of HIV-1 capsid uncoating revealed by single-molecule analysis
eLife, 2018 Uncoating of the metastable HIV-1 capsid is a tightly regulated disassembly process required for release of the viral cDNA prior to nuclear import. To understand the intrinsic capsid disassembly pathway and how it can be modulated, we have developed a ...
Chantal L Márquez +10 more
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