Results 21 to 30 of about 424,913 (262)
Non-invasive chimeric HaloTag labeling to study clustering and diffusion of membrane proteins
STAR Protocols, 2022 Summary: As live imaging plays an increasingly critical role in cell biology research, the desire to label and track individual protein molecules in vivo has been growing.
Yiran Chang, Daniel J. Dickinson
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STAR Protocols, 2021 Summary: Co-immunoprecipitation (co-IP) of protein complexes from cell lysates is widely used to study protein-protein interactions. However, establishing robust co-IP assays often involves considerable optimization.
Christoph J. Burckhardt +2 more
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High-Throughput Single-Molecule Transcription Assay [PDF]
Biophysical Journal, 2012 The study of the role and mechanics of proteins in a cell is crucial to understanding the complexity of cells. Most biological systems respond to a change by adding heterogeneity to its population states. One of the most effective ways of studying a biological system is to compare the population states of proteins as a function of time and chemical ...
Kim, Soohong +5 more
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Optimized protocol for single-molecule RNA FISH to visualize gene expression in S. cerevisiae
STAR Protocols, 2021 Summary: Single-molecule RNA fluorescence in situ hybridization (smFISH) allows subcellular visualization, localization, and quantification of endogenous RNA molecules in fixed cells.
Heta P. Patel +2 more
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Optical Tweezers to Force Information out of Biological and Synthetic Systems One Molecule at a Time
Biophysica, 2022 Over the last few decades, in vitro single-molecule manipulation techniques have enabled the use of force and displacement as controlled variables in biochemistry. Measuring the effect of mechanical force on the real-time kinetics of a biological process
Rebeca Bocanegra +4 more
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DNA Y Structure: A Versatile, Multidimensional Single Molecule Assay [PDF]
Nano Letters, 2014 Optical trapping is a powerful single molecule technique used to study dynamic biomolecular events, especially those involving DNA and DNA-binding proteins. Current implementations usually involve only one of stretching, unzipping, or twisting DNA along one dimension.
Inman, James T. +6 more
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STAR Protocols, 2022 Summary: Multiplexed single-molecule magnetic tweezers (MT) have recently been employed to probe the RNA synthesis dynamics of RNA-dependent RNA polymerases (RdRp).
Louis Kuijpers +3 more
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A functional single-molecule binding assay via force spectroscopy [PDF]
Proceedings of the National Academy of Sciences, 2007 Protein–ligand interactions, including protein–protein interactions, are ubiquitously essential in biological processes and also have important applications in biotechnology. A wide range of methodologies have been developed for quantitative analysis of protein–ligand interactions.
Yi, Cao +3 more
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Prospects for single-molecule electrostatic detection in molecular motor gliding motility assays
New Journal of Physics, 2021 Molecular motor gliding motility assays based on myosin/actin or kinesin/microtubules are of interest for nanotechnology applications ranging from cargo-trafficking in lab-on-a-chip devices to novel biocomputation strategies.
M Sanchez Miranda +5 more
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Frontiers in Microbiology, 2022 The F1-ATPase is a rotary motor fueled by ATP hydrolysis. Its rotational dynamics have been well characterized using single-molecule rotation assays. While F1-ATPases from various species have been studied using rotation assays, the standard model for ...
Hiroyuki Noji, Hiroshi Ueno
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