Results 21 to 30 of about 6,253,377 (301)

Single molecule probing of disordered RNA binding proteins

STAR Protocols, 2022
Summary: Liquid-liquid phase separation of intrinsically disordered proteins is known to underlie diverse pathologies such as neurodegeneration, cancer, and aging.
Kevin Rhine, Sua Myong
doaj   +1 more source

High-Throughput Single-Molecule Transcription Assay [PDF]

Biophysical Journal, 2012
The study of the role and mechanics of proteins in a cell is crucial to understanding the complexity of cells. Most biological systems respond to a change by adding heterogeneity to its population states. One of the most effective ways of studying a biological system is to compare the population states of proteins as a function of time and chemical ...
Kim, Soohong, Streets, Aaron M., Lin, Ron R., Quake, Stephen R., Weiss, Shimon, Majumdar, Devdoot S.   +5 more
openaire   +1 more source

Non-invasive chimeric HaloTag labeling to study clustering and diffusion of membrane proteins

STAR Protocols, 2022
Summary: As live imaging plays an increasingly critical role in cell biology research, the desire to label and track individual protein molecules in vivo has been growing.
Yiran Chang, Daniel J. Dickinson
doaj   +1 more source

Co-immunoprecipitation and semi-quantitative immunoblotting for the analysis of protein-protein interactions

STAR Protocols, 2021
Summary: Co-immunoprecipitation (co-IP) of protein complexes from cell lysates is widely used to study protein-protein interactions. However, establishing robust co-IP assays often involves considerable optimization.
Christoph J. Burckhardt, John D. Minna, Gaudenz Danuser   +2 more
doaj   +1 more source

songuno1/single-molecule-assay: Single-molecule assay analysis

, 2023
Single-molecule assay analysis tool for "Transcriptional pause extension benefits the stand-by rather than catch-up Rho-dependent termination"
Eunho Song
core   +1 more source

Optimized protocol for single-molecule RNA FISH to visualize gene expression in S. cerevisiae

STAR Protocols, 2021
Summary: Single-molecule RNA fluorescence in situ hybridization (smFISH) allows subcellular visualization, localization, and quantification of endogenous RNA molecules in fixed cells.
Heta P. Patel, Ineke Brouwer, Tineke L. Lenstra   +2 more
doaj   +1 more source

Optical Tweezers to Force Information out of Biological and Synthetic Systems One Molecule at a Time

Biophysica, 2022
Over the last few decades, in vitro single-molecule manipulation techniques have enabled the use of force and displacement as controlled variables in biochemistry. Measuring the effect of mechanical force on the real-time kinetics of a biological process
Rebeca Bocanegra, María Ortiz-Rodríguez, Ismael Plaza Garcia-Abadillo, Carlos R-Pulido, Borja Ibarra   +4 more
doaj   +1 more source

Identification of binding mechanisms in single molecule-DNA complexes [PDF]

, 2003
Eckel R, Ros R, Ros A, Wilking SD, Sewald N, Anselmetti D. Identification of binding mechanisms in single molecule-DNA complexes. Biophysical journal. 2003;85(3):1968-1973.Changes in the elastic properties of single deoxyribonucleic acid (DNA) molecules ...
Anselmetti, Dario, Wilking, Sven David, Ros, Robert, Eckel, Rainer, Sven David Wilking, Dario Anselmetti, Robert Ros, Wilking, S.D., Sewald, Norbert ; https://orcid.org/, Ra Ros, Ros, Alexandra, Sewald, Norbert, Rainer Eckel, Anselmetti, Dario ; https://orcid.org/, Y Norbert Sewald   +14 more
core   +1 more source

Visualizing helicases unwinding DNA at the single molecule level. [PDF]

, 2010
DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free energy from ATP hydrolysis. Single molecule approaches enable us to address detailed mechanistic questions about how such enzymes ...
Fili, Natali, Mashanov, Gregory I, Batters, C, Dillingham, MS, Yeeles, Joseph T P, Toseland, Christopher P, Mashanov, GI, Toseland, CP, Wallace, Mark I, Webb, Martin R, Molloy, JE, Batters, Christopher, Webb, MR, Wallace, Mark, Fili, N, Dillingham, Mark S, Yeeles, JT, Molloy, Justin E   +17 more
core   +1 more source

Single-molecule nano-optics at low temperature [PDF]

, 2006
The work presented in this thesis involves experiments on single molecules at low temperature. At low temperature, many disturbing temperature activated processes are frozen out, resulting in extremely sharp zero-phonon lines in the fluorescence ex ...
Butter, Jacqueline Yvonne Paula
core   +1 more source