Results 31 to 40 of about 6,253,377 (301)
STAR Protocols, 2022 Summary: Multiplexed single-molecule magnetic tweezers (MT) have recently been employed to probe the RNA synthesis dynamics of RNA-dependent RNA polymerases (RdRp).
Louis Kuijpers +3 more
doaj +1 more source
A functional single-molecule binding assay via force spectroscopy [PDF]
Proceedings of the National Academy of Sciences, 2007 Protein–ligand interactions, including protein–protein interactions, are ubiquitously essential in biological processes and also have important applications in biotechnology. A wide range of methodologies have been developed for quantitative analysis of protein–ligand interactions.
Yi, Cao +3 more
openaire +2 more sources
Single-molecule chemical denaturation of riboswitches [PDF]
, 2013 To date, single-molecule RNA science has been developed almost exclusively around the effect of metal ions as folding promoters and stabilizers of the RNA structure.
St-Pierre, Patrick +14 more
core +1 more source
Prospects for single-molecule electrostatic detection in molecular motor gliding motility assays
New Journal of Physics, 2021 Molecular motor gliding motility assays based on myosin/actin or kinesin/microtubules are of interest for nanotechnology applications ranging from cargo-trafficking in lab-on-a-chip devices to novel biocomputation strategies.
M Sanchez Miranda +5 more
doaj +1 more source
Future of biomedical sciences: Single molecule microscopy [PDF]
, 2007 The behavior of single molecule defines whether a cell lives, dies, or responds to a specific drug treatment. Single molecule microscopies have begun to reveal the number, location, and functionalities of molecules outside and inside living cells.
Walter, Nils G.
core +1 more source
Frontiers in Microbiology, 2022 The F1-ATPase is a rotary motor fueled by ATP hydrolysis. Its rotational dynamics have been well characterized using single-molecule rotation assays. While F1-ATPases from various species have been studied using rotation assays, the standard model for ...
Hiroyuki Noji, Hiroshi Ueno
doaj +1 more source
Single molecule force spectroscopy on ligand-DNA complexes: from molecular binding mechanisms to biosensor applications [PDF]
, 2004 Ros R, Eckel R, Bartels FW, et al. Single molecule force spectroscopy on ligand-DNA complexes: from molecular binding mechanisms to biosensor applications. Journal of Biotechnology.
Baumgarth, Birgit +9 more
core +1 more source
FLAMBE: A kinetic fluorescence polarization assay to study activation of monomeric BAX
STAR Protocols, 2022 Summary: BAX activation techniques are crucial to studying the intrinsic pathway of apoptosis— thousands of pro-apoptotic signals converge on BAX activation.
Jarvier N. Mohammed +2 more
doaj +1 more source
DNA Y Structure: A Versatile, Multidimensional Single Molecule Assay [PDF]
Nano Letters, 2014 Optical trapping is a powerful single molecule technique used to study dynamic biomolecular events, especially those involving DNA and DNA-binding proteins. Current implementations usually involve only one of stretching, unzipping, or twisting DNA along one dimension.
Inman, James T. +6 more
openaire +2 more sources
Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]
, 2013 The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Fili, Natalia +7 more
core +1 more source