Results 31 to 40 of about 479,986 (210)
Multiplexed Small Molecule Ligand Binding Assays by Affinity Labeling and DNA Sequence Analysis
, 2021 Small molecule binding assays to target proteins are a core component of drug discovery and development. While a number of assay formats are available, significant drawbacks still remain in cost, sensitivity, and throughput.
Krusemark, Casey J. +3 more
core +1 more source
Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]
, 2013 The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Fili, Natalia +7 more
core +1 more source
Cytoplasmic Actin: Purification and Single Molecule Assembly Assays [PDF]
, 2013 The actin cytoskeleton is essential to all eukaryotic cells. In addition to playing important structural roles, assembly of actin into filaments powers diverse cellular processes, including cell motility, cytokinesis, and endocytosis. Actin polymerization is tightly regulated by its numerous cofactors, which control spatial and temporal assembly of ...
Scott D, Hansen +2 more
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A SARS‐CoV‐2 Neutralization Assay Using Single Molecule Arrays [PDF]
Angewandte Chemie International Edition, 2021 AbstractCoronavirus disease 2019 (COVID‐19) manifests with high clinical variability and warrants sensitive and specific assays to analyze immune responses in infected and vaccinated individuals. Using Single Molecule Arrays (Simoa), we developed an assay to assess antibody neutralization with high sensitivity and multiplexing capabilities based on ...
Tal Gilboa +12 more
openaire +2 more sources
STAR Protocols, 2022 Summary: Multiplexed single-molecule magnetic tweezers (MT) have recently been employed to probe the RNA synthesis dynamics of RNA-dependent RNA polymerases (RdRp).
Louis Kuijpers +3 more
doaj +1 more source
Focus on function: Single molecule RNA enzymology [PDF]
, 2007 The ability of RNA to catalyze chemical reactions was first demonstrated 25 years ago with the discovery that group I introns and RNase P function as RNA enzymes (ribozymes).
Mark A. Ditzler +7 more
core +1 more source
Prospects for single-molecule electrostatic detection in molecular motor gliding motility assays
New Journal of Physics, 2021 Molecular motor gliding motility assays based on myosin/actin or kinesin/microtubules are of interest for nanotechnology applications ranging from cargo-trafficking in lab-on-a-chip devices to novel biocomputation strategies.
M Sanchez Miranda +5 more
doaj +1 more source
Frontiers in Microbiology, 2022 The F1-ATPase is a rotary motor fueled by ATP hydrolysis. Its rotational dynamics have been well characterized using single-molecule rotation assays. While F1-ATPases from various species have been studied using rotation assays, the standard model for ...
Hiroyuki Noji, Hiroshi Ueno
doaj +1 more source
Single-Molecule Optical Biosensing:Recent Advances and Future Challenges [PDF]
, 2023 In recent years, the sensitivity and specificity of optical sensors has improved tremendously due to improvements in biochemical functionalization protocols and optical detection systems.
Dey, Swayandipta +2 more
core +1 more source
A single molecule assay for measuring site-specific DNA cleavage [PDF]
Analytical Biochemistry, 2016 Sequence-specific DNA cleavage is a key step in a number of genomic transactions. Here, we report a single-molecule technique that allows the simultaneous measurement of hundreds of DNAs, thereby collecting significant statistics in a single experiment. Microbeads are tethered with single DNA molecules in a microfluidic channel.
Stefano, Gambino +5 more
openaire +2 more sources