Results 31 to 40 of about 424,913 (262)
FLAMBE: A kinetic fluorescence polarization assay to study activation of monomeric BAX
STAR Protocols, 2022 Summary: BAX activation techniques are crucial to studying the intrinsic pathway of apoptosis— thousands of pro-apoptotic signals converge on BAX activation.
Jarvier N. Mohammed +2 more
doaj +1 more source
A single molecule assay for measuring site-specific DNA cleavage [PDF]
Analytical Biochemistry, 2016 Sequence-specific DNA cleavage is a key step in a number of genomic transactions. Here, we report a single-molecule technique that allows the simultaneous measurement of hundreds of DNAs, thereby collecting significant statistics in a single experiment. Microbeads are tethered with single DNA molecules in a microfluidic channel.
Stefano, Gambino +5 more
openaire +2 more sources
Advanced Sensor Research, 2022 The ultrasensitive measurement of protein markers plays a pivotal role in the early diagnosis of infectious and progressive diseases. Recently, digital methods such as those enabled by the Simoa Planar Array technology (SP‐X System) have made significant
Cecilia Scandurra +5 more
doaj +1 more source
A framework to validate fluorescently labeled DNA-binding proteins for single-molecule experiments
Cell Reports: Methods, 2023 Summary: Due to the enhanced labeling capability of maleimide-based fluorescent probes, lysine-cysteine-lysine (KCK) tags are frequently added to proteins for visualization. In this study, we employed an in vitro single-molecule DNA flow-stretching assay
Miranda Molina +7 more
doaj +1 more source
STAR ProtocolsSummary: OpuA is an osmoregulatory ATP-binding cassette transporter that undergoes different conformations upon varying salt concentrations and ligand conditions.
Lyan van der Sleen +4 more
doaj +1 more source
Fast Thermocycling in Custom Microfluidic Cartridge for Rapid Single-Molecule Droplet PCR
Sensors, 2023 The microfluidic droplet polymerase chain reaction (PCR), which enables simultaneous DNA amplification in numerous droplets, has led to the discovery of various applications that were previously deemed unattainable.
Hirokazu Takahara +2 more
doaj +1 more source
Strength in numbers: quantitative single‐molecule
WIREs Developmental Biology, 2015 Gene expression is a fundamental process that underlies development, homeostasis, and behavior of organisms. The fact that it relies on nucleic acid intermediates, which can specifically interact with complementary probes, provides an excellent opportunity for studying the multiple steps—transcription, RNA processing, transport, translation ...
Gaspar, Imre, Ephrussi, Anne
openaire +2 more sources
A flow extension tethered particle motion assay for single-molecule proteolysis [PDF]
Biochemistry, 2019 Abstract Regulated proteolysis of signaling proteins under mechanical tension enables cells to communicate with their environment in a variety of developmental and physiologic contexts. The role of force in inducing proteolytic sensitivity has been explored using magnetic tweezers at the single-molecule level with bead-tethered assays ...
Andrew A. Drabek +2 more
openaire +3 more sources
Multiplex Single-Molecule DNA Barcoding Using an Oligonucleotide Ligation Assay [PDF]
Biophysical Journal, 2018 Detection of specific nucleic acid sequences is invaluable in biological studies such as genetic disease diagnostics and genome profiling. Here we developed a highly sensitive and specific detection method that combines an advanced oligonucleotide ligation assay (OLA) with multicolor single-molecule fluorescence.
Severins, Ivo +2 more
openaire +2 more sources
STAR ProtocolsSummary: For single-molecule studies requiring surface anchoring of biomolecules, poorly passivated surfaces can result in alterations of biomolecule structure and function that lead to artifacts.
Tung T. Le +5 more
doaj +1 more source