Results 51 to 60 of about 479,986 (210)

Implementation of Experimental Design Techniques to Optimize Immunoglobulins Detection with Ultrasensitive Sandwich Immunoassays

Advanced Sensor Research, 2022
The ultrasensitive measurement of protein markers plays a pivotal role in the early diagnosis of infectious and progressive diseases. Recently, digital methods such as those enabled by the Simoa Planar Array technology (SP‐X System) have made significant
Cecilia Scandurra, Paolo Bollella, Ronald Österbacka, Francesco Leonetti, Eleonora Macchia, Luisa Torsi   +5 more
doaj   +1 more source

Fast Thermocycling in Custom Microfluidic Cartridge for Rapid Single-Molecule Droplet PCR

Sensors, 2023
The microfluidic droplet polymerase chain reaction (PCR), which enables simultaneous DNA amplification in numerous droplets, has led to the discovery of various applications that were previously deemed unattainable.
Hirokazu Takahara, Hayato Tanaka, Masahiko Hashimoto   +2 more
doaj   +1 more source

Elucidation of the Mechanism of an Epigenetic Switch by Single-molecule Assays [PDF]

Biophysical Journal, 2009
The lambda bacteriophage epigenetic switch determines the growth lifestyle of the virus after infection of its host (E. coli). It is now clear that the switch consists of a ∼2.3 kbp-long DNA loop mediated by the lambda repressor protein. Using tethered particle microscopy (TPM), magnetic tweezers and AFM, our laboratory has novel, direct evidence of ...
Finzi, Laura, Zurla, Chiara, Manzo, Carlo, Dunlap, David   +3 more
openaire   +1 more source

DNA Flow-Stretch Assays for Studies of Protein-DNA Interactions at the Single-Molecule Level

Applied Nano, 2022
Protein-DNA interactions are the core of the cell’s molecular machinery. For a long time, conventional biochemical methods served as a powerful investigatory basis of protein-DNA interactions and target search mechanisms.
Aurimas Kopūstas, Mindaugas Zaremba, Marijonas Tutkus   +2 more
doaj   +1 more source

DNA Y Structure: A Multidimensional Single Molecule Assay [PDF]

Biophysical Journal, 2014
Single molecule techniques have enabled significant advancement in the understanding of biological systems. However, current optical trapping techniques allow for force, extension and torque to be measured only along the axis of applied tension. This one dimensional aspect limits studies of complex biological systems due to the difficulty of data ...
Inman, James, Smith, Ben, Hall, Michael, Forties, Robert A., Wang, Michelle D.   +4 more
openaire   +1 more source

Direct experimental observation of blue-light-induced conformational change and intermolecular interactions of cryptochrome

Communications Biology, 2022
Refined structures, protein-docking analysis and single molecule assays provides insights into light-induced conformational changes in the cryptochrome CraCRY.
Pei Li, Huaqiang Cheng, Vikash Kumar, Cecylia Severin Lupala, Xuanxuan Li, Yingchen Shi, Chongjun Ma, Keehyoung Joo, Jooyoung Lee, Haiguang Liu, Yan-Wen Tan   +10 more
doaj   +1 more source

Protocol for effective surface passivation for single-molecule studies of chromatin and topoisomerase II

STAR Protocols
Summary: For single-molecule studies requiring surface anchoring of biomolecules, poorly passivated surfaces can result in alterations of biomolecule structure and function that lead to artifacts.
Tung T. Le, Xiang Gao, Seong Ha Park, Jaeyoon Lee, James T. Inman, Michelle D. Wang   +5 more
doaj   +1 more source

A Toolbox for Site-Specific Labeling of RecQ Helicase With a Single Fluorophore Used in the Single-Molecule Assay

Frontiers in Molecular Biosciences, 2020
Fluorescently labeled proteins can improve the detection sensitivity and have been widely used in a variety of biological measurements. In single-molecule assays, site-specific labeling of proteins enables the visualization of molecular interactions ...
Fang-Yuan Teng, Fang-Yuan Teng, Fang-Yuan Teng, Fang-Yuan Teng, Zong-Zhe Jiang, Zong-Zhe Jiang, Ling-Yun Huang, Man Guo, Feng Chen, Xi-Miao Hou, Xu-Guang Xi, Xu-Guang Xi, Yong Xu, Yong Xu   +13 more
doaj   +1 more source

Identification of single-point mutations in mycobacterial 16S rRNA sequences by confocal single-molecule fluorescence spectroscopy

, 2006
Marme N, Friedrich A, Müller M, et al. Identification of single-point mutations in mycobacterial 16S rRNA sequences by confocal single-molecule fluorescence spectroscopy. Nucleic Acids Research.
Friedrich, Achim, Sauer, Markus, Nolte, Oliver, Wolfrum, Jürgen, Marme, Nicole, Knemeyer, Jens-Peter, Müller, Matthias, Hoheisel, Jörg D.   +7 more
core   +1 more source

Pseudotype-based neutralization assays for influenza: a systematic analysis [PDF]

, 2015
The use of vaccination against the influenza virus remains the most effective method of mitigating the significant morbidity and mortality caused by this virus.
Ferrara, Francesca, Keith eGrehan, Temperton, Nigel J., George William Carnell, Craig Peter Thompson, Francesca eFerrara, Nigel James Temperton, Grehan, Keith, Carnell, George W., Thompson, Craig P., Francesca Ferrara, Keith Grehan   +11 more
core   +1 more source