Results 51 to 60 of about 6,253,377 (301)
Fast Thermocycling in Custom Microfluidic Cartridge for Rapid Single-Molecule Droplet PCR
Sensors, 2023 The microfluidic droplet polymerase chain reaction (PCR), which enables simultaneous DNA amplification in numerous droplets, has led to the discovery of various applications that were previously deemed unattainable.
Hirokazu Takahara +2 more
doaj +1 more source
A flow extension tethered particle motion assay for single-molecule proteolysis [PDF]
Biochemistry, 2019 Abstract Regulated proteolysis of signaling proteins under mechanical tension enables cells to communicate with their environment in a variety of developmental and physiologic contexts. The role of force in inducing proteolytic sensitivity has been explored using magnetic tweezers at the single-molecule level with bead-tethered assays ...
Andrew A. Drabek +2 more
openaire +3 more sources
Multiplex Single-Molecule DNA Barcoding Using an Oligonucleotide Ligation Assay [PDF]
Biophysical Journal, 2018 Detection of specific nucleic acid sequences is invaluable in biological studies such as genetic disease diagnostics and genome profiling. Here we developed a highly sensitive and specific detection method that combines an advanced oligonucleotide ligation assay (OLA) with multicolor single-molecule fluorescence.
Severins, Ivo +2 more
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STAR ProtocolsSummary: For single-molecule studies requiring surface anchoring of biomolecules, poorly passivated surfaces can result in alterations of biomolecule structure and function that lead to artifacts.
Tung T. Le +5 more
doaj +1 more source
Communications Biology, 2022 Refined structures, protein-docking analysis and single molecule assays provides insights into light-induced conformational changes in the cryptochrome CraCRY.
Pei Li +10 more
doaj +1 more source
A Single‐Molecule Liposome Assay for Membrane Permeabilization
Angewandte ChemieAbstract Cell membrane disruption is associated with numerous diseases and underlies the activity of various antimicrobial agents. The rapid screening of compounds capable of disrupting or permeabilizing biological membranes is essential to the search for new therapeutic drugs.
Bąk, Krzysztof M. +9 more
openaire +2 more sources
Single Molecule Fluorescence Assay of Alpha Synuclein Dimerization [PDF]
Biophysical Journal, 2015 The aggregation of α-Synuclein (α-Syn) is linked to Parkinson's disease. The mechanism of early aggregation steps and the effect of pathogenic single-point mutations remain elusive. We report here a single molecule fluorescence study of α-Syn dimerization and the effect of pathological single point mutations.
Lv, Zhengjian +6 more
openaire +1 more source
Multiplexed Small Molecule Ligand Binding Assays by Affinity Labeling and DNA Sequence Analysis [PDF]
, 2021 Small molecule binding assays to target proteins are a core component of drug discovery and development. While a number of assay formats are available, significant drawbacks still remain in cost, sensitivity, and throughput.
Krusemark, Casey J. +3 more
core +1 more source
Protocol to quantify mechanical properties of cells using optical tweezers
STAR ProtocolsSummary: Mechanical properties of cells determine their ability to deform when subjected to force, which is crucial in many biological processes. Here, we present a protocol to quantify these properties on living cells by applying piconewton-range forces
Wessel S. Rodenburg, Jorine M. Eeftens
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Frontiers in Molecular Biosciences, 2020 Fluorescently labeled proteins can improve the detection sensitivity and have been widely used in a variety of biological measurements. In single-molecule assays, site-specific labeling of proteins enables the visualization of molecular interactions ...
Fang-Yuan Teng +13 more
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