Results 1 to 10 of about 807,043 (313)

Protocol for the isolation and immunoprecipitation of cell surface proteins from Drosophila melanogaster pupae

open access: yesSTAR Protocols
Summary: Transmembrane proteins are notoriously difficult to isolate from tissue samples due to their hydrophobic nature and often low abundance. Here, we describe a protocol for the isolation, enrichment, and detection of the high-molecular-weight ...
Anne Bormann, Marina Bigl, Nicole Scholz
doaj   +1 more source

Author Correction: True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy

open access: yesNature Communications, 2019
In the original version of this Article, equation three contained a sign error whereby the term RT was added when it should have been subtracted. This has now been corrected in the PDF and HTML versions of the Article.
Christophe Jung   +6 more
doaj   +1 more source

The dynamics and functional impact of tRNA repertoires during early embryogenesis in zebrafish

open access: yesThe EMBO Journal
Embryogenesis entails dramatic shifts in mRNA translation and turnover that reprogram gene expression during cellular proliferation and differentiation.
Madalena M Reimão-Pinto   +5 more
doaj   +1 more source

Development of Biolayer Interferometry (BLI)-Based Double-Stranded RNA Detection Method with Application in mRNA-Based Therapeutics and Vaccines

open access: yesPharmaceutics
Background: In vitro-transcribed (IVT) mRNA has been established as a promising platform for therapeutics and vaccine development. Double-stranded RNA (dsRNA) is a major impurity of IVT mRNA and can trigger unfavored immune responses, potentially causing
Dharia Sara Silas   +12 more
doaj   +1 more source

Isolation, Characterization and IgE Binding of Two 2S Albumins of Pomegranate Seeds

open access: yesFoods
Literature reports suggest that the presence of proteins in pomegranate seeds is responsible for sensitization and IgE-mediated allergic reactions.
Lisa Tuppo   +6 more
doaj   +1 more source

Applications of sequence coevolution in membrane protein biochemistry. [PDF]

open access: yesBiochim Biophys Acta Biomembr, 2018
Nicoludis JM, Gaudet R.
europepmc   +1 more source

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