Results 41 to 50 of about 479,986 (210)
FLAMBE: A kinetic fluorescence polarization assay to study activation of monomeric BAX
STAR Protocols, 2022 Summary: BAX activation techniques are crucial to studying the intrinsic pathway of apoptosis— thousands of pro-apoptotic signals converge on BAX activation.
Jarvier N. Mohammed +2 more
doaj +1 more source
Strength in numbers: quantitative single‐molecule
WIREs Developmental Biology, 2015 Gene expression is a fundamental process that underlies development, homeostasis, and behavior of organisms. The fact that it relies on nucleic acid intermediates, which can specifically interact with complementary probes, provides an excellent opportunity for studying the multiple steps—transcription, RNA processing, transport, translation ...
Gaspar, Imre, Ephrussi, Anne
openaire +2 more sources
A flow extension tethered particle motion assay for single-molecule proteolysis [PDF]
Biochemistry, 2019 Abstract Regulated proteolysis of signaling proteins under mechanical tension enables cells to communicate with their environment in a variety of developmental and physiologic contexts. The role of force in inducing proteolytic sensitivity has been explored using magnetic tweezers at the single-molecule level with bead-tethered assays ...
Andrew A. Drabek +2 more
openaire +3 more sources
Multiplex Single-Molecule DNA Barcoding Using an Oligonucleotide Ligation Assay [PDF]
Biophysical Journal, 2018 Detection of specific nucleic acid sequences is invaluable in biological studies such as genetic disease diagnostics and genome profiling. Here we developed a highly sensitive and specific detection method that combines an advanced oligonucleotide ligation assay (OLA) with multicolor single-molecule fluorescence.
Severins, Ivo +2 more
openaire +2 more sources
Single‐Molecule Binding Assay Using Nanopores and Dimeric NP Conjugates [PDF]
Advanced Materials, 2021 AbstractThe ability to measure biomarkers, both specifically and selectively at the single‐molecule level in biological fluids, has the potential to transform the diagnosis, monitoring, and therapeutic intervention of diseases. The use of nanopores has been gaining prominence in this area, not only for sequencing but more recently in screening ...
Ren Ren +8 more
openaire +3 more sources
FRET Assay for Single Molecule Measurements of Troponin Activation [PDF]
Biophysical Journal, 2013 We seek to understand the energetics and design principle of the TnTmA7 switch and the basis for cooperative activation. Single molecule studies provide the needed resolution, revealing conformational substates in a model-independent manner. Multiple single-cysteine mutants of cardiac TnI (cTnI) and cardiac TnC (cTnC) were engineered to generate an ...
Moutsoglou, Maria E. +2 more
openaire +1 more source
Single Molecule Fluorescence Assay of Alpha Synuclein Dimerization [PDF]
Biophysical Journal, 2015 The aggregation of α-Synuclein (α-Syn) is linked to Parkinson's disease. The mechanism of early aggregation steps and the effect of pathogenic single-point mutations remain elusive. We report here a single molecule fluorescence study of α-Syn dimerization and the effect of pathological single point mutations.
Lv, Zhengjian +6 more
openaire +1 more source
A framework to validate fluorescently labeled DNA-binding proteins for single-molecule experiments
Cell Reports: Methods, 2023 Summary: Due to the enhanced labeling capability of maleimide-based fluorescent probes, lysine-cysteine-lysine (KCK) tags are frequently added to proteins for visualization. In this study, we employed an in vitro single-molecule DNA flow-stretching assay
Miranda Molina +7 more
doaj +1 more source
Identification of binding mechanisms in single molecule-DNA complexes
, 2003 Eckel R, Ros R, Ros A, Wilking SD, Sewald N, Anselmetti D. Identification of binding mechanisms in single molecule-DNA complexes. Biophysical journal. 2003;85(3):1968-1973.Changes in the elastic properties of single deoxyribonucleic acid (DNA) molecules ...
Anselmetti, Dario +14 more
core +1 more source
STAR ProtocolsSummary: OpuA is an osmoregulatory ATP-binding cassette transporter that undergoes different conformations upon varying salt concentrations and ligand conditions.
Lyan van der Sleen +4 more
doaj +1 more source