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Single molecule detection for in vitro diagnostics
, 2022 Paper 68620HIn this paper we present a novel highly sensitive detection system for diagnostic applications. The system is designed to meet the needs of medical diagnostics for reliable measurements of pathogens and biomarkers in the low concentration ...
Greiner, B. +7 more
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, 2022 All supplementary code for "Rapid single-molecule characterisation of DNA ...
StefanHMueller
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Focus on function: Single molecule RNA enzymology [PDF]
, 2007 The ability of RNA to catalyze chemical reactions was first demonstrated 25 years ago with the discovery that group I introns and RNase P function as RNA enzymes (ribozymes).
Mark A. Ditzler +7 more
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A single molecule assay for measuring site-specific DNA cleavage [PDF]
Analytical Biochemistry, 2016 Sequence-specific DNA cleavage is a key step in a number of genomic transactions. Here, we report a single-molecule technique that allows the simultaneous measurement of hundreds of DNAs, thereby collecting significant statistics in a single experiment. Microbeads are tethered with single DNA molecules in a microfluidic channel.
Stefano, Gambino +5 more
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Real-Time analysis and visualization for single-molecule based super-resolution microscopy [PDF]
, 2013 Accurate multidimensional localization of isolated fluorescent emitters is a time consuming process in single-molecule based super-resolution microscopy.
Jean-Baptiste Sibarita +9 more
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A SARS‐CoV‐2 Neutralization Assay Using Single Molecule Arrays [PDF]
Angewandte Chemie International Edition, 2021 AbstractCoronavirus disease 2019 (COVID‐19) manifests with high clinical variability and warrants sensitive and specific assays to analyze immune responses in infected and vaccinated individuals. Using Single Molecule Arrays (Simoa), we developed an assay to assess antibody neutralization with high sensitivity and multiplexing capabilities based on ...
Tal Gilboa +12 more
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STAR ProtocolsSummary: OpuA is an osmoregulatory ATP-binding cassette transporter that undergoes different conformations upon varying salt concentrations and ligand conditions.
Lyan van der Sleen +4 more
doaj +1 more source
Advanced Sensor Research, 2022 The ultrasensitive measurement of protein markers plays a pivotal role in the early diagnosis of infectious and progressive diseases. Recently, digital methods such as those enabled by the Simoa Planar Array technology (SP‐X System) have made significant
Cecilia Scandurra +5 more
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A framework to validate fluorescently labeled DNA-binding proteins for single-molecule experiments
Cell Reports: Methods, 2023 Summary: Due to the enhanced labeling capability of maleimide-based fluorescent probes, lysine-cysteine-lysine (KCK) tags are frequently added to proteins for visualization. In this study, we employed an in vitro single-molecule DNA flow-stretching assay
Miranda Molina +7 more
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Strength in numbers: quantitative single‐molecule
WIREs Developmental Biology, 2015 Gene expression is a fundamental process that underlies development, homeostasis, and behavior of organisms. The fact that it relies on nucleic acid intermediates, which can specifically interact with complementary probes, provides an excellent opportunity for studying the multiple steps—transcription, RNA processing, transport, translation ...
Gaspar, Imre, Ephrussi, Anne
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