Results 41 to 50 of about 424,913 (262)

A Toolbox for Site-Specific Labeling of RecQ Helicase With a Single Fluorophore Used in the Single-Molecule Assay

Frontiers in Molecular Biosciences, 2020
Fluorescently labeled proteins can improve the detection sensitivity and have been widely used in a variety of biological measurements. In single-molecule assays, site-specific labeling of proteins enables the visualization of molecular interactions ...
Fang-Yuan Teng, Fang-Yuan Teng, Fang-Yuan Teng, Fang-Yuan Teng, Zong-Zhe Jiang, Zong-Zhe Jiang, Ling-Yun Huang, Man Guo, Feng Chen, Xi-Miao Hou, Xu-Guang Xi, Xu-Guang Xi, Yong Xu, Yong Xu   +13 more
doaj   +1 more source

Direct experimental observation of blue-light-induced conformational change and intermolecular interactions of cryptochrome

Communications Biology, 2022
Refined structures, protein-docking analysis and single molecule assays provides insights into light-induced conformational changes in the cryptochrome CraCRY.
Pei Li, Huaqiang Cheng, Vikash Kumar, Cecylia Severin Lupala, Xuanxuan Li, Yingchen Shi, Chongjun Ma, Keehyoung Joo, Jooyoung Lee, Haiguang Liu, Yan-Wen Tan   +10 more
doaj   +1 more source

Using single-molecule fluorescence in situ hybridization and immunohistochemistry to count RNA molecules in single cells in zebrafish embryos

STAR Protocols, 2023
Summary: Taming gene expression variability is critical for robust pattern formation during embryonic development. Here, we describe an optimized protocol for single-molecule fluorescence in situ hybridization and immunohistochemistry in zebrafish ...
Kemal Keseroglu, Oriana Q.H. Zinani, Ertuğrul M. Özbudak   +2 more
doaj   +1 more source

FRET Assay for Single Molecule Measurements of Troponin Activation [PDF]

Biophysical Journal, 2013
We seek to understand the energetics and design principle of the TnTmA7 switch and the basis for cooperative activation. Single molecule studies provide the needed resolution, revealing conformational substates in a model-independent manner. Multiple single-cysteine mutants of cardiac TnI (cTnI) and cardiac TnC (cTnC) were engineered to generate an ...
Moutsoglou, Maria E., Kim, Gi-Ho, Robinson, John M.   +2 more
openaire   +1 more source

Protocol to quantify mechanical properties of cells using optical tweezers

STAR Protocols
Summary: Mechanical properties of cells determine their ability to deform when subjected to force, which is crucial in many biological processes. Here, we present a protocol to quantify these properties on living cells by applying piconewton-range forces
Wessel S. Rodenburg, Jorine M. Eeftens
doaj   +1 more source

Assessing reversible and irreversible binding effects of kinase covalent inhibitors through ADP-Glo assays

STAR Protocols, 2021
Summary: Typical enzymatic inhibition assays often demonstrate improved potency for kinase covalent inhibitors compared to reversible inhibitors. This can primarily be attributed to the irreversible mode of action and could affect the evaluations of the ...
Martin Schröder, Apirat Chaikuad
doaj   +1 more source

A Single‐Molecule Liposome Assay for Membrane Permeabilization

Angewandte Chemie
Abstract Cell membrane disruption is associated with numerous diseases and underlies the activity of various antimicrobial agents. The rapid screening of compounds capable of disrupting or permeabilizing biological membranes is essential to the search for new therapeutic drugs.
Bąk, Krzysztof M., Edwards, Daniel C., George, Dylan, Singh, Bhanu, Ferguson, Ryan, Zhao, Tianxiao, Piché, Kristin, Louwrier, Ariel, Cockroft, Scott L., Horrocks, Mathew H.   +9 more
openaire   +2 more sources

European Standard Clinical Practice Guideline and EXPeRT Recommendations for the Diagnosis and Management of Gastroenteropancreatic Neuroendocrine Neoplasms in Children and Adolescents

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Pediatric gastroenteropancreatic neuroendocrine neoplasms (GEP‐NENs) are extremely rare and clinically heterogeneous. Management has largely been extrapolated from adult practice. This European Standard Clinical Practice Guideline (ESCP), developed by the EXPeRT network in collaboration with adult NEN experts, provides (adult) evidence ...
Michaela Kuhlen, Calogero Virgone, Antje Redlich, Michael Abele, Ricardo Lopez‐Almaraz, Tal Ben‐Ami, Ewa Bien, Gianni Bisogno, Maja Mazic Cesen, Andrea Ferrari, Malgorzata A. Krawczyk, Yves Reguerre, Dominik T. Schneider, Ines B. Brecht, Charlotte Rigaud, Bilgehan Yalcin, Rainer Claus, Christian Vokuhl, Constantin Lapa, Jörg Fuchs, Segolene Hescot, Thomas Walter, Julien Hadoux, Daniel Orbach   +23 more
wiley   +1 more source

Protocol for single-molecule imaging of transcription and epigenetic factors in human neural stem cell-derived neurons

STAR Protocols
Summary: Single-molecule imaging (SMI) is a powerful approach to quantify the spatiotemporal dynamics of transcription in living cells. Here, we describe a protocol of SMI for transcription and epigenetic factors in human cortical neurons derived from ...
Yuri Atsumi, Nobuhiko Yamamoto, Noriyuki Sugo   +2 more
doaj   +1 more source

Single Molecule Fluorescence Assay of Alpha Synuclein Dimerization [PDF]

Biophysical Journal, 2015
The aggregation of α-Synuclein (α-Syn) is linked to Parkinson's disease. The mechanism of early aggregation steps and the effect of pathogenic single-point mutations remain elusive. We report here a single molecule fluorescence study of α-Syn dimerization and the effect of pathological single point mutations.
Lv, Zhengjian, Krasnoslobodtsev, Alexey V., Zhang, Yuliang, Ysselstein, Daniel, Rochet, Jean-Christophe, Blanchard, Scott, Lyubchenko, Yuri L.   +6 more
openaire   +1 more source