Results 61 to 70 of about 6,253,377 (301)

Using single-molecule fluorescence in situ hybridization and immunohistochemistry to count RNA molecules in single cells in zebrafish embryos

STAR Protocols, 2023
Summary: Taming gene expression variability is critical for robust pattern formation during embryonic development. Here, we describe an optimized protocol for single-molecule fluorescence in situ hybridization and immunohistochemistry in zebrafish ...
Kemal Keseroglu, Oriana Q.H. Zinani, Ertuğrul M. Özbudak   +2 more
doaj   +1 more source

FRET Assay for Single Molecule Measurements of Troponin Activation [PDF]

Biophysical Journal, 2013
We seek to understand the energetics and design principle of the TnTmA7 switch and the basis for cooperative activation. Single molecule studies provide the needed resolution, revealing conformational substates in a model-independent manner. Multiple single-cysteine mutants of cardiac TnI (cTnI) and cardiac TnC (cTnC) were engineered to generate an ...
Moutsoglou, Maria E., Kim, Gi-Ho, Robinson, John M.   +2 more
openaire   +1 more source

Single molecules in fluorescence fluctuation spectroscopy: Effective volume and photon counting histogram

, 2022
Paper 686204This paper presents a stochastic theory for the interpretation of photon counting histograms in fluorescence fluctuation spectroscopy (FFS). New concepts of an effective volume and a single molecule probability distribution are introduced to ...
Greiner, B., Ackermann, J., Mathis, H.
core   +1 more source

European Standard Clinical Practice Guideline and EXPeRT Recommendations for the Diagnosis and Management of Gastroenteropancreatic Neuroendocrine Neoplasms in Children and Adolescents

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Pediatric gastroenteropancreatic neuroendocrine neoplasms (GEP‐NENs) are extremely rare and clinically heterogeneous. Management has largely been extrapolated from adult practice. This European Standard Clinical Practice Guideline (ESCP), developed by the EXPeRT network in collaboration with adult NEN experts, provides (adult) evidence ...
Michaela Kuhlen, Calogero Virgone, Antje Redlich, Michael Abele, Ricardo Lopez‐Almaraz, Tal Ben‐Ami, Ewa Bien, Gianni Bisogno, Maja Mazic Cesen, Andrea Ferrari, Malgorzata A. Krawczyk, Yves Reguerre, Dominik T. Schneider, Ines B. Brecht, Charlotte Rigaud, Bilgehan Yalcin, Rainer Claus, Christian Vokuhl, Constantin Lapa, Jörg Fuchs, Segolene Hescot, Thomas Walter, Julien Hadoux, Daniel Orbach   +23 more
wiley   +1 more source

Protocol for single-molecule imaging of transcription and epigenetic factors in human neural stem cell-derived neurons

STAR Protocols
Summary: Single-molecule imaging (SMI) is a powerful approach to quantify the spatiotemporal dynamics of transcription in living cells. Here, we describe a protocol of SMI for transcription and epigenetic factors in human cortical neurons derived from ...
Yuri Atsumi, Nobuhiko Yamamoto, Noriyuki Sugo   +2 more
doaj   +1 more source

Assessing reversible and irreversible binding effects of kinase covalent inhibitors through ADP-Glo assays

STAR Protocols, 2021
Summary: Typical enzymatic inhibition assays often demonstrate improved potency for kinase covalent inhibitors compared to reversible inhibitors. This can primarily be attributed to the irreversible mode of action and could affect the evaluations of the ...
Martin Schröder, Apirat Chaikuad
doaj   +1 more source

Identification of single-point mutations in mycobacterial 16S rRNA sequences by confocal single-molecule fluorescence spectroscopy [PDF]

, 2006
Marme N, Friedrich A, Müller M, et al. Identification of single-point mutations in mycobacterial 16S rRNA sequences by confocal single-molecule fluorescence spectroscopy. Nucleic Acids Research.
Friedrich, Achim, Sauer, Markus, Nolte, Oliver, Wolfrum, Jürgen, Marme, Nicole, Knemeyer, Jens-Peter, Müller, Matthias, Hoheisel, Jörg D.   +7 more
core   +1 more source

Solid Pseudopapillary Neoplasm of the Pancreas in Children and Adolescents: Expert Recommendations

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Solid pseudopapillary neoplasm of the pancreas (SPN) is a rare low‐grade malignant exocrine pancreatic tumor, mostly discovered during the second decade of life in females, with a very good prognosis, provided microscopically complete surgical excision is achieved.
Sabine Irtan, Michael Abele, Calogero Virgone, Michaela Kuhlen, Ricardo Lopez‐Almarez, Tal Ben‐Ami, Ewa Bien, Maja Cesen, Malgorzata A. Krawczyk, Andrea Ferrari, Gianni Bisogno, Yves Reguerre, Dominik T. Schneider, Louise Galmiche, Jorg Fuchs, Sarah Braungart, Bilgehan Yalcin, Ines B. Brecht, Daniel Orbach   +18 more
wiley   +1 more source

Single-Molecule-Based Electroluminescent Device as Future White Light Source

, 2019
During the last two decades, spectacular development of light-emitting diodes (LEDs) has been achieved owing to their widespread application possibilities.
Shie-Ming Peng (1425703), Michitoshi Hayashi (1321539), Krishna Prasad Bera (5058050), Batjargal Sainbileg (4866403), Gene-Hsiang Lee (1470748), Muhammad Usman (1504405), Golam Haider (1437523), Kuang-Lieh Lu (1504399), Yang-Fang Chen (1437529)   +8 more
core   +2 more sources

Protocol to prepare doubly labeled fluorescent nucleosomes for single-molecule fluorescence microscopy

STAR Protocols, 2023
Summary: Single-molecule fluorescence microscopy (SMFM) has been shown to be informative in understanding the interaction of chromatin-associated factors with nucleosomes, the basic building unit of chromatin.
Mohamed Ghoneim, Catherine A. Musselman
doaj   +1 more source