Results 61 to 70 of about 479,986 (210)

A Single‐Molecule Liposome Assay for Membrane Permeabilization

Angewandte Chemie
Abstract Cell membrane disruption is associated with numerous diseases and underlies the activity of various antimicrobial agents. The rapid screening of compounds capable of disrupting or permeabilizing biological membranes is essential to the search for new therapeutic drugs.
Bąk, Krzysztof M., Edwards, Daniel C., George, Dylan, Singh, Bhanu, Ferguson, Ryan, Zhao, Tianxiao, Piché, Kristin, Louwrier, Ariel, Cockroft, Scott L., Horrocks, Mathew H.   +9 more
openaire   +2 more sources

Protocol to quantify mechanical properties of cells using optical tweezers

STAR Protocols
Summary: Mechanical properties of cells determine their ability to deform when subjected to force, which is crucial in many biological processes. Here, we present a protocol to quantify these properties on living cells by applying piconewton-range forces
Wessel S. Rodenburg, Jorine M. Eeftens
doaj   +1 more source

Using single-molecule fluorescence in situ hybridization and immunohistochemistry to count RNA molecules in single cells in zebrafish embryos

STAR Protocols, 2023
Summary: Taming gene expression variability is critical for robust pattern formation during embryonic development. Here, we describe an optimized protocol for single-molecule fluorescence in situ hybridization and immunohistochemistry in zebrafish ...
Kemal Keseroglu, Oriana Q.H. Zinani, Ertuğrul M. Özbudak   +2 more
doaj   +1 more source

Protocol for single-molecule imaging of transcription and epigenetic factors in human neural stem cell-derived neurons

STAR Protocols
Summary: Single-molecule imaging (SMI) is a powerful approach to quantify the spatiotemporal dynamics of transcription in living cells. Here, we describe a protocol of SMI for transcription and epigenetic factors in human cortical neurons derived from ...
Yuri Atsumi, Nobuhiko Yamamoto, Noriyuki Sugo   +2 more
doaj   +1 more source

Assessing reversible and irreversible binding effects of kinase covalent inhibitors through ADP-Glo assays

STAR Protocols, 2021
Summary: Typical enzymatic inhibition assays often demonstrate improved potency for kinase covalent inhibitors compared to reversible inhibitors. This can primarily be attributed to the irreversible mode of action and could affect the evaluations of the ...
Martin Schröder, Apirat Chaikuad
doaj   +1 more source

Protocol to prepare doubly labeled fluorescent nucleosomes for single-molecule fluorescence microscopy

STAR Protocols, 2023
Summary: Single-molecule fluorescence microscopy (SMFM) has been shown to be informative in understanding the interaction of chromatin-associated factors with nucleosomes, the basic building unit of chromatin.
Mohamed Ghoneim, Catherine A. Musselman
doaj   +1 more source

Single-molecule studies of exonuclease proteins

, 2021
DNA replication and maintenance are an integral part of the continued survival of all life on this planet. Therefore, the collection of proteins and the mechanisms they employ to maintain and copy DNA in the presence of continuous damage experienced from
Ryan Edward Muir (20168301)
core  

Double Digital Assay for Single Extracellular Vesicle and Single Molecule Detection

Advanced Science, 2023
AbstractExtracellular vesicles (EVs) have emerged as a promising source of biomarkers for disease diagnosis. However, current diagnostic methods for EVs present formidable challenges, given the low expression levels of biomarkers carried by EV samples, as well as their complex physical and biological properties.
David E. Reynolds, Menghan Pan, Jingbo Yang, George Galanis, Yoon Ho Roh, Renee‐Tyler T. Morales, Shailesh Senthil Kumar, Su‐Jin Heo, Xiaowei Xu, Wei Guo, Jina Ko   +10 more
openaire   +3 more sources

Protocol to generate fast-dissociating recombinant antibody fragments for multiplexed super-resolution microscopy

STAR Protocols, 2023
Summary: Multiplexed high-density label super-resolution microscopy image reconstruction by integrating exchangeable single-molecule localization (IRIS) enables elucidating fine structures and molecular distribution in cells and tissues.
Qianli Zhang, Akitoshi Miyamoto, Naoki Watanabe   +2 more
doaj   +1 more source

Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery

, 2013
Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to
Bhavyashree Suresh, Ferhan Ayaydin, Bokros, Attila, Eszter Molnár, Peksel, Begüm, Ágnes Medgyesi, Liliána Fehér, Pradeep Kumar Reddy Cingaram, Puskás, László G., Soujanya Kuntam, Attila Bokros, Begüm Peksel, Gabriella Fábián, Molnár, Eszter, László G. Puskás, Kuntam, Soujanya, Liliána Z. Fehér, Fábián, Gabriella, László Puskás, Cingaram, Pradeep Kumar Reddy, Pradeep Cingaram   +20 more
core   +1 more source