Results 81 to 90 of about 479,986 (210)
Single-molecule assay reveals strand switching and enhanced processivity of UvrD [PDF]
Proceedings of the National Academy of Sciences, 2004 DNA helicases are enzymes capable of unwinding double-stranded DNA (dsDNA) to provide the single-stranded DNA template required in many biological processes. Among these, UvrD, an essential DNA repair enzyme, has been shown to unwind dsDNA while moving 3′-5′ on one strand.
Dessinges, M.-N. +4 more
openaire +3 more sources
Discordant correlation between serological assays observed when measuring heterosubtypic responses against avian influenza H5 and H7 viruses in unexposed individuals [PDF]
, 2014 The human population is constantly exposed to multiple influenza A subtypes due to zoonotic spill over and rapid viral evolution driven by intrinsic error-prone replication and immunological pressure.
Ferrara, Francesca +5 more
core +1 more source
Multiplexed single-molecule flow-stretching bead assay for DNA enzymology
BMB Reports, 2019 Single-molecule techniques have been used successfully to visualize real-time enzymatic activities, revealing transient complex properties and heterogeneity of various biological events. Especially, conventional force spectroscopy including optical tweezers and magnetic tweezers has been widely used to monitor change in DNA length by enzymes with high ...
Ryanggeun Lee +2 more
openaire +4 more sources
, 2023 The Mre11/Rad50 (MR) complex of the DNA damage repair system is a first-responder to double-stranded DNA breaks, an extremely serious form of DNA damage. Rad50 is an ABC ATPase that energizes the nuclease activity of Mre11.
Theilen, Karsten
core
D1.4 Report on the development of single molecule force assays
, 2023 This report describes progress in the development of single molecule force assays.
openaire +2 more sources
Ultrasensitive Automatic Detection of Small Molecules by Membrane Imaging of Single Molecule Assays
Determination of trace amounts of targets or even a single molecule target has always been a challenge in the detection field. Digital measurement methods established for single molecule counting of proteins, such as single molecule arrays (Simoa) or ...
Jialei Bai (4180990) +11 more
core +2 more sources
STAR ProtocolsSummary: Female Ae. aegypti mosquitoes utilize midgut proteolytic enzymes to digest blood meal proteins, but activity detection has largely depended on the use of chromogenic substrates.
Abigail G. Ramirez +4 more
doaj +1 more source
Single-Molecule Analysis of MicroRNA and Logic Operations Using a Smart Plasmonic Nanobiosensor
, 2018 Analysis of biomolecules at the single-molecule level is a great challenge in molecular diagnostics, gene profiling, and environmental monitoring. In this work, we design a smart plasmonic nanobiosensor based on individual Au@Ag core–shell nanocube (Au ...
Wei Huang (36889) +13 more
core +1 more source
Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection
PLoS ONE, 2013 Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed a digital proximity ligation assay for protein ...
Ke R +4 more
openaire +6 more sources
STAR ProtocolsSummary: Recombinant microtubule (MT)-severing proteins are valuable for studying their mechanisms of action; however, purifying them in an active state is challenging. Here, we provide a protocol to obtain biologically active and highly pure recombinant
Asumi Hoshino +4 more
doaj +1 more source