Results 101 to 110 of about 6,253,377 (301)

Single-molecule studies of exonuclease proteins

, 2021
DNA replication and maintenance are an integral part of the continued survival of all life on this planet. Therefore, the collection of proteins and the mechanisms they employ to maintain and copy DNA in the presence of continuous damage experienced from
Ryan Edward Muir (20168301)
core   +1 more source

Protocol for the detection of proteolytic activity in female Aedes aegypti mosquito midgut extracts using fluorogenic AMC protease substrates

STAR Protocols
Summary: Female Ae. aegypti mosquitoes utilize midgut proteolytic enzymes to digest blood meal proteins, but activity detection has largely depended on the use of chromogenic substrates.
Abigail G. Ramirez, Daniel Fong, My Anh Le, Jun Isoe, Alberto A. Rascón, Jr.   +4 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Protocol for purifying biologically active microtubule-severing protein UNC-45A from E.coli using GST-affinity and spin columns

STAR Protocols
Summary: Recombinant microtubule (MT)-severing proteins are valuable for studying their mechanisms of action; however, purifying them in an active state is challenging. Here, we provide a protocol to obtain biologically active and highly pure recombinant
Asumi Hoshino, Nimisha Krishnan, Mihir Shetty, Jennifer L. Ross, Martina Bazzaro   +4 more
doaj   +1 more source

Advances in optical single-molecule detection: On the road to super-sensitive bioaffinity assays [PDF]

, 2020
The ability to detect low concentrations of analytes and in particular low‐abundance biomarkers is of fundamental importance, e.g., for early‐stage disease diagnosis.
Gorris, Hans H., Mickert, Matthias J., Pastucha, Matěj, Farka, Zdeněk   +3 more
core   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Single Molecule Fluorescence Imaging of Nanoconfinement in Porous Materials [PDF]

Single-molecule fluorescence (SMF) microscopy imaging was employed to understand the nanoconfinement in porous materials. The changes in chemical and physical properties of molecules in nanoconfined space are often termed as the confinement effects.
Mansour, Nourhan
core   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

Protocol for the simultaneous detection of nuclear long non-coding RNAs and proteins in human iPSC-derived cardiomyocytes

STAR Protocols
Summary: Nuclear-enriched long non-coding RNAs (lncRNAs) can regulate gene expression by interacting with specific RNA-binding proteins (RBPs). Here, we present a protocol to directly image these lncRNAs at the single-molecule level together with their ...
Giulia Buonaiuto, Tiziana Santini, Monica Ballarino   +2 more
doaj   +1 more source

Insight into the biochemical mechanism of DNA helicases provided by bulk-phase and single-molecule assays. [PDF]

Methods, 2022
Bianco PR.
europepmc   +1 more source