Results 91 to 100 of about 479,986 (210)

Insight into the biochemical mechanism of DNA helicases provided by bulk-phase and single-molecule assays. [PDF]

Methods, 2022
Bianco PR.
europepmc   +1 more source

Single-molecule analysis of DNA replication in Xenopus egg extracts

, 2012
The recent advent in single-molecule imaging and manipulation methods has made a significant impact on the understanding of molecular mechanisms underlying many essential cellular processes.
Hasan Yardimci (8128839), Antonius van Oijen (19587070), Anna B Loveland (20213976), Johannes C Walter (17762397)   +3 more
core  

Protocol for the simultaneous detection of nuclear long non-coding RNAs and proteins in human iPSC-derived cardiomyocytes

STAR Protocols
Summary: Nuclear-enriched long non-coding RNAs (lncRNAs) can regulate gene expression by interacting with specific RNA-binding proteins (RBPs). Here, we present a protocol to directly image these lncRNAs at the single-molecule level together with their ...
Giulia Buonaiuto, Tiziana Santini, Monica Ballarino   +2 more
doaj   +1 more source

Total Internal Reflection Fluorescence (TIRF) Single-Molecule Assay to Analyze the Motility of Kinesin

Bio-Protocol
The motile parameters of kinesin superfamily proteins are fundamental to intracellular transport. Single-molecule motility assays using total internal reflection fluorescence (TIRF) microscopy are a gold standard technique for measuring the motile ...
Tomoki Kita, Shinsuke Niwa
doaj   +1 more source

Simultaneous detection of the spike and nucleocapsid proteins from SARS-CoV-2 based on ultrasensitive single molecule assays. [PDF]

Anal Bioanal Chem, 2021
Cai Q, Mu J, Lei Y, Ge J, Aryee AA, Zhang X, Li Z.   +6 more
europepmc   +1 more source

Ultrasensitive quantification of cardiac troponin I by a Single Molecule Counting method: analytical validation and biological features

, 2018
AIM: To evaluate analytical and biological characteristics of the Singulex Clarity® cTnI assay, based upon Single Molecule Counting technology. METHODS: Assay's analytical sensitivity, precision, linearity, hook effect, cross-reactivity or interference
Monsalve, L., Ordonez-Llanos, J., Grau-Agramunt, M., Garcia-Osuna, A., Estis, J., Nolan, N., Bishop, J., Gaze D.C., Bartolome, A., Sandlund, J., Livingston, R., Telha, C., Morris, T.   +12 more
core   +1 more source

A novel high???throughput single???molecule technique: DNA curtain

, 2021
Single-molecule (SM) techniques have been contributing to unraveling the detailed mechanisms underlying DNA metabolic processes with superior spatiotemporal resolution beyond the limit of traditional biochemical assays.
Lee, Ja Yil, Ja Yil Lee, Cha, JongJin
core   +1 more source

Single-Molecule G-Quadruplex Nanopore Assay [PDF]

Biophysical Journal, 2020
Filip N. Boskovic, Jinbo Zhu, Kaikai Chen, Ulrich F. Keyser   +3 more
openaire   +1 more source

High-throughput single-molecule studies of protein–DNA interactions

, 2014
Fluorescence and force-based single-molecule studies of protein–nucleic acid interactions continue to shed critical insights into many aspects of DNA and RNA processing.
Finkelstein, Ilya J., Robison, Aaron D., Aaron D. Robison, Ilya J. Finkelstein   +3 more
core   +1 more source

Single-molecule detection on a portable 3D-printed microscope

Nature Communications, 2019
Single-molecule in vitro assays require dedicated confocal microscopes equipped with fluorescence correlation spectroscopy (FCS) modules. Here the authors present a compact, cheap and open-source 3D-printed confocal microscope for single photon counting ...
James W. P. Brown, Arnaud Bauer, Mark E Polinkovsky, Akshay Bhumkar, Dominic J. B. Hunter, Katharina Gaus, Emma Sierecki, Yann Gambin   +7 more
doaj   +1 more source