Results 111 to 120 of about 479,986 (210)

Tracking enzymatic steps of DNA topoisomerases using single-molecule micromanipulation [PDF]

, 2002
In this article, we describe single-molecule assays using magnetic traps and we applied these assays to topoisomerase enzymes which unwind and disentangle DNA molecules.
Charvin, G., Dekker, N. H., Jean-François Allemand, Gilles Charvin, Allemand, J. F., Nynke H. Dekker, Strick, T. R., Terence R. Strick, Vincent Croquette, Bensimon, D., David Bensimon, Croquette, V.   +11 more
core   +1 more source

Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope

Nature Communications
Detecting single molecules, which represents the ultimate level of sensitivity, is typically achieved with research-grade equipment. Here we present a low-cost, portable smartphone-based fluorescence microscope capable of detecting single-molecule ...
Morgane Loretan, Mariano Barella, Nathan Fuchs, Samet Kocabey, Karol Kołątaj, Fernando D. Stefani, Guillermo P. Acuna   +6 more
doaj   +1 more source

Single molecule array measures of LRRK2 kinase activity in serum link Parkinson’s disease severity to peripheral inflammation

Molecular Neurodegeneration
Background LRRK2-targeting therapeutics that inhibit LRRK2 kinase activity have advanced to clinical trials in idiopathic Parkinson’s disease (iPD). LRRK2 phosphorylates Rab10 on endolysosomes in phagocytic cells to promote some types of immunological ...
Yuan Yuan, Huizhong Li, Kashyap Sreeram, Tuyana Malankhanova, Ravindra Boddu, Samuel Strader, Allison Chang, Nicole Bryant, Talene A. Yacoubian, David G. Standaert, Madalynn Erb, Darren J. Moore, Laurie H. Sanders, Michael W. Lutz, Dmitry Velmeshev, Andrew B. West   +15 more
doaj   +1 more source

Studying glycobiology at the single-molecule level

, 2018
Attempts to elucidate the roles of carbohydrate-associated structures in biology have led to the distinct field of glycobiology research. The focus of this field has been in understanding the evolution, biosynthesis and interactions of glycans, both ...
Davis, BG, Lakshminarayanan, A, Richard, M, Davis, Benjamin   +3 more
core   +1 more source

Single-molecule FRET studies in live bacteria

, 2016
Single-molecule fluorescence and single-molecule Förster resonance energy transfer (FRET) have proven enormously successful in understanding molecular and cellular processes over the last two decades.
Plochowietz, Anne, Anne Plochowietz
core   +1 more source

Hopping of a processivity factor on DNA revealed by single-molecule assays of diffusion

, 2019
Many DNA-interacting proteins diffuse on DNA to perform their biochemical functions. Processivity factors diffuse on DNA to permit unimpeded elongation by their associated DNA polymerases, but little is known regarding their rates and mechanisms of ...
Komazin-Meredith, Gloria, Golan, David E., Oijen, Antoine van, Mirchev, Rossen, Coen, Donald M.   +4 more
core   +1 more source

Protocol for assembling photostable fluorescence-labeled tiny DNA origami blocks to observe kinesin cooperation

STAR Protocols
Summary: Fluorescence-labeled tiny DNA origami blocks (FTOBs) enable observation of kinesin motility and their cooperation with minimal blinking and photobleaching. Here, we present a protocol for assembling FTOBs to observe kinesin cooperation.
Tomoki Kita, Ryota Sugie, Yuki Suzuki, Shinsuke Niwa   +3 more
doaj   +1 more source

Development of novel bioanalytical assays with single-molecule readout for biomarker detection and drug candidate characterization

, 2017
Bioanalytical assays with single-molecule readout for studying molecular interactions have in the past decades received increasing attention. The high sensitivity often offered by this readout scheme has for example enabled ultra-sensitive analyte ...
Wahlsten, Olov
core  

Stretching and immobilization of DNA for studies of protein–DNA interactions at the single-molecule level

Nanoscale Research Letters, 2007
Single-molecule studies of the interactions of DNA and proteins are important in a variety of biological or biotechnology processes ranging from the protein’s search for its DNA target site, DNA replication, transcription, or repair, and genome ...
Dukkipati VenkatRam, Pang Stella, Kim JiHoon, Larson Ronald   +3 more
doaj   +1 more source

Fluorescence resonance energy transfer studies of protein interactions

, 2008
This thesis presents an investigation of fluorescence resonance energy transfer (FRET) as a reporting signal for protein-protein interactions. Quantitative optical assays to measure protein binding, conjugation and deconjugation are developed and results
Martin, Sarah Friede
core