Results 131 to 140 of about 6,253,377 (301)

Recent Advance in Single-Molecule Fluorescent Biosensors for Tumor Biomarker Detection

Biosensors
The construction of biosensors for specific, sensitive, and rapid detection of tumor biomarkers significantly contributes to biomedical research and early cancer diagnosis.
Jie Zhang, Jiawen Liu, Lixue Qiao, Qian Zhang, Juan Hu, Chun-yang Zhang   +5 more
doaj   +1 more source

Single-molecule detection on a portable 3D-printed microscope

Nature Communications, 2019
Single-molecule in vitro assays require dedicated confocal microscopes equipped with fluorescence correlation spectroscopy (FCS) modules. Here the authors present a compact, cheap and open-source 3D-printed confocal microscope for single photon counting ...
James W. P. Brown, Arnaud Bauer, Mark E Polinkovsky, Akshay Bhumkar, Dominic J. B. Hunter, Katharina Gaus, Emma Sierecki, Yann Gambin   +7 more
doaj   +1 more source

Metal-Single-Molecule-Semiconductor Junctions Formed by a Radical Reaction Bridging Gold and Silicon Electrodes.

, 2019
Here we report molecular films terminated with diazonium salts moieties at both ends which enables single-molecule contacts between gold and silicon electrodes at open circuit via a radical reaction.
Yan B Vogel (23524978), Simone Ciampi (23286055), Nadim Darwish (23287990), Ismael Díez-Pérez (2478700), Michelle L Coote (2219656), Anton P Le Brun (20233710), Chandramalika R Peiris (23524975), Albert C Aragonès (23524981)   +7 more
core  

Single-molecule-resolution ultrafast near-field optical microscopy via plasmon lifetime extension

, 2020
A recent study shows that: when a long lifetime particle is positioned near a plasmonic metal nanoparticle, lifetime of plasmon oscillations extends, but, "only" near that long-life particle [PRB 101, 035416 (2020)].
Ramazan Sahin (14358723), Rasim Volga Ovali (17516628), Mehmet Emre Tasgin (14358444), Alpan Bek (253144)   +3 more
core   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Single molecule array measures of LRRK2 kinase activity in serum link Parkinson’s disease severity to peripheral inflammation

Molecular Neurodegeneration
Background LRRK2-targeting therapeutics that inhibit LRRK2 kinase activity have advanced to clinical trials in idiopathic Parkinson’s disease (iPD). LRRK2 phosphorylates Rab10 on endolysosomes in phagocytic cells to promote some types of immunological ...
Yuan Yuan, Huizhong Li, Kashyap Sreeram, Tuyana Malankhanova, Ravindra Boddu, Samuel Strader, Allison Chang, Nicole Bryant, Talene A. Yacoubian, David G. Standaert, Madalynn Erb, Darren J. Moore, Laurie H. Sanders, Michael W. Lutz, Dmitry Velmeshev, Andrew B. West   +15 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Single-molecule analysis of DNA replication in Xenopus egg extracts

, 2012
The recent advent in single-molecule imaging and manipulation methods has made a significant impact on the understanding of molecular mechanisms underlying many essential cellular processes.
Hasan Yardimci (8128839), Antonius van Oijen (19587070), Anna B Loveland (20213976), Johannes C Walter (17762397)   +3 more
core   +1 more source

Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope

Nature Communications
Detecting single molecules, which represents the ultimate level of sensitivity, is typically achieved with research-grade equipment. Here we present a low-cost, portable smartphone-based fluorescence microscope capable of detecting single-molecule ...
Morgane Loretan, Mariano Barella, Nathan Fuchs, Samet Kocabey, Karol Kołątaj, Fernando D. Stefani, Guillermo P. Acuna   +6 more
doaj   +1 more source