Results 121 to 130 of about 479,986 (210)

Competitive Assays of Label-Free DNA Hybridization with Single-Molecule Fluorescence Imaging Detection

open access: yes, 2016
Single-molecule imaging of fluorescently labeled biomolecules is a powerful technique for measuring association interactions; however, care must be taken to ensure that the fluorescent labels do not influence the system being probed.
Eric M. Peterson (1430326)   +2 more
core   +1 more source

Detection of low frequency FGFR3 mutations in the urine of bladder cancer patients using next-generation deep sequencing

open access: yesResearch and Reports in Urology, 2012
John M Millholland, Shuqiang Li, Cecilia A Fernandez, Anthony P ShuberPredictive Biosciences Inc, Lexington, MA, USAAbstract: Biological fluid-based noninvasive biomarker assays for monitoring and diagnosing disease are clinically powerful.
Millholl   +4 more
doaj  

Mechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate. [PDF]

open access: yesNat Commun, 2017
Lee YK   +6 more
europepmc   +1 more source

Surfing on a new wave of single-molecule fluorescence methods

open access: yes, 2010
Hohlbein J, Gryte K, Heilemann M, Kapanidis AN. Surfing on a new wave of single-molecule fluorescence methods. PHYSICAL BIOLOGY. 2010;7(3): 31001.Single-molecule fluorescence microscopy is currently one of the most popular methods in the single-molecule ...
Gryte, Kristofer   +11 more
core   +1 more source

Confocal laser scanning microscopy of nanoparticles applied to immunosorbent assays

open access: yes, 2011
The aim of this project was to demonstrate and develop a confocal readout method for fluorescent immunosorbent assays and investigate its potential advantages in comparison to traditional immunoassays.
Ghafari, H
core  

Dual-wavelength metalens enables Epi-fluorescence detection from single molecules

open access: yesNature Communications
Single molecule fluorescence spectroscopy is at the heart of molecular biophysics research and the most sensitive biosensing assays. The growing demand for precision medicine and environmental monitoring requires the creation of miniaturized and portable
Aleksandr Barulin   +6 more
doaj   +1 more source

Development of novel bioanalytical assays with single-molecule readout for biomarker detection and drug candidate characterization [Elektronisk resurs]

open access: yes, 2017
Bioanalytical assays with single-molecule readout for studying molecular interactions have in the past decades received increasing attention. The high sensitivity often offered by this readout scheme has for example enabled ultra-sensitive analyte ...
Wahlsten, Olov,
core  

Single-molecule studies of replication-transcription conflicts and recombinational DNA repair

open access: yes
DNA replication, transcription, and recombinational repair are essential processes that maintain the genomic integrity of bacterial cells by ensuring high-fidelity DNA duplication, accurate gene expression, and efficient DNA repair, thereby preventing ...
Nischal Sharma (19583728)
core   +1 more source

Single-molecule visualization of fast polymerase turnover in the bacterial replisome

open access: yes, 2017
The Escherichia coli DNA replication machinery has been used as a road map to uncover design rules that enable DNA duplication with high efficiency and fidelity.
Slobodan Jergic (20120775)   +10 more
core  

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