Results 71 to 80 of about 479,986 (210)
Single-Molecule Assay for Proteolytic Susceptibility: Force-Induced Collagen Destabilization [PDF]
Biophysical Journal, 2018 Force plays a key role in regulating dynamics of biomolecular structure and interactions, yet techniques are lacking to manipulate and continuously read out this response with high throughput. We present an enzymatic assay for force-dependent accessibility of structure that makes use of a wireless mini-radio centrifuge force microscope to provide a ...
Michael W H, Kirkness, Nancy R, Forde
openaire +2 more sources
STAR ProtocolsSummary: Here, we present a protocol for single-molecule super-resolution imaging of the nuclear export of pre-ribosomal subunits pre-40S and pre-60S through nuclear pore complexes. We describe steps for plating cells and co-transfecting cells.
Mark Tingey +3 more
doaj +1 more source
An automated Bayesian pipeline for rapid analysis of single-molecule binding data
Nature Communications, 2019 Analysis of single-molecule binding assays still requires substantial manual user intervention. Here, the authors present a pipeline for rapid, automated analysis of co-localization single-molecule spectroscopy images, with a modular user interface that ...
Carlas S. Smith +5 more
doaj +1 more source
Observing protein dynamics during DNA-lesion bypass by the replisome
Frontiers in Molecular Biosciences, 2022 Faithful DNA replication is essential for all life. A multi-protein complex called the replisome contains all the enzymatic activities required to facilitate DNA replication, including unwinding parental DNA and synthesizing two identical daughter ...
Elise M. Wilkinson +5 more
doaj +1 more source
Development of Quantitative Intensity-Based Single-Molecule Assays
, 2021 Fluorescence microscopy has emerged as a popular and powerful tool within biology research, owing to its exceptional signal contrast, specificity, and the versatility of the various microscope designs.
Croop, Benjamin
core
Immunofunctional assay of human growth hormone (hGH) in serum: A possible consensus for quantitative hGH measurement [PDF]
, 1996 Confirmation of the diagnosis of GH deficiency in adults and children involves provocative testing for human (h) GH. Different commercially available immunoassays yield largely discrepant results in the measurement of GH levels in human serum.
Strasburger, Christian J. +3 more
core +1 more source
Rho-dependent transcription termination proceeds via three routes
Nature Communications, 2022 Rho is a general transcription termination factor in bacteria. Here, Song et al. use single-molecule fluorescence assays to provide evidence that Rho-mediated transcription termination can occur via three kinetically different routes.
Eunho Song +7 more
doaj +1 more source
Single-Molecule Imaging in Living Plant Cells: A Methodological Review
, 2021 Single-molecule imaging is emerging as a revolutionary approach to studying fundamental questions in plants. However, compared with its use in animals, the application of single-molecule imaging in plants is still underexplored.
Ai-Yu Guo +5 more
core +1 more source
Single-molecule and single-particle imaging of molecular motors in vitro and in vivo.
, 2014 Motor proteins are multi-potent molecular machines, whose localisation, function and regulation are achieved through tightly controlled processes involving conformational changes and interactions with their tracks, cargos and binding partners ...
Natalia Fili (17157790)
core +1 more source
, 2016 We have detected single-molecule binding interactions of a target peptide with the calcium-signaling protein calmodulin (CaM) immobilized in an agarose gel, and we have demonstrated the application of a single-molecule binding assay to measure the ...
Carey K. Johnson (472525) +2 more
core +1 more source