Results 11 to 20 of about 479,986 (210)
Single-molecule assays for investigating protein misfolding and aggregation
Physical Chemistry Chemical Physics, 2013 Protein misfolding and aggregation are relevant to many fields. Recently, their investigation has experienced a revival as a central topic in the research of numerous human diseases, including Parkinson's and Alzheimer's.
Hoffmann, Armin +2 more
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Assaying chromosomal inversions by single-molecule haplotyping [PDF]
Nature Methods, 2006 Inversions are an important form of structural variation, but they are difficult to characterize, as their breakpoints often fall within inverted repeats. We have developed a method called 'haplotype fusion' in which an inversion breakpoint is genotyped by performing fusion PCR on single molecules of human genomic DNA.
Turner, D J +6 more
openaire +3 more sources
Direct single-molecule imaging for diagnostic and blood screening assays [PDF]
Proceedings of the National Academy of Sciences, 2021 Significance Direct imaging and counting single molecules captured on magnetic microparticles enables diagnostic assays with ultrahigh sensitivities and wide linear detection ranges. The method offers simple assay protocols, short incubation times, minimal reagent consumption, and does not require enzymatic signal amplification.
Qiaoqiao Ruan +3 more
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STAR Protocols, 2022 Summary: DNA fiber combing is a versatile technique that provides insight into replication fork dynamics at single-molecule resolution. DNA fibers are bound to silanized coverslips and combed, which straightens and aligns the fibers along a single axis ...
Gemma Moore +2 more
doaj +1 more source
A single-molecule assay for telomerase structure-function analysis [PDF]
Nucleic Acids Research, 2009 The activity of the telomerase ribonucleoprotein enzyme is essential for the maintenance of genome stability and normal cell development. Despite the biomedical importance of telomerase activity, detailed structural models for the enzyme remain to be established.
Wu, John Yanyun +2 more
openaire +4 more sources
Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup
STAR Protocols, 2022 Summary: Aquaporins (AQPs) are water channels embedded in the cell membrane that are critical in maintaining water homeostasis. We describe a protocol for determining the water permeation capacity of AQPs reconstituted into proteoliposomes.
Jonas Hyld Steffen +7 more
doaj +1 more source
Nanopatterning at the Service of Single Molecule Assays [PDF]
Biophysical Journal, 2010 Today, the manipulation and integration of objects with nanometric dimensions is essential for a great number of applications. In biology and medicine, the study of structural dynamics in individual molecules or other key cellular processes is often limited by the low throughput of current methods.
Cerf, Aline, Vieu, Christophe
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Two-parameter single-molecule analysis for measurement of chromatin mobility
STAR Protocols, 2020 Summary: This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to
Jonathan Lerner +5 more
doaj +1 more source
STAR Protocols, 2022 Summary: Single-molecule tracking (SMT) is a powerful approach to quantify the biophysical parameters of protein dynamics in live cells. Here, we describe a protocol for SMT in live cells of the budding yeast Saccharomyces cerevisiae.
Nitesh Kumar Podh +4 more
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STAR Protocols, 2021 Summary: This protocol provides instructions to track the global dynamics of single epigenetic regulatory factors in live cells. We describe an approach to generate cell lines that stably express HaloTag-fused proteins.
Kyle Brown, Samantha Kent, Xiaojun Ren
doaj +1 more source