Results 11 to 20 of about 424,913 (262)

Assaying chromosomal inversions by single-molecule haplotyping [PDF]

Nature Methods, 2006
Inversions are an important form of structural variation, but they are difficult to characterize, as their breakpoints often fall within inverted repeats. We have developed a method called 'haplotype fusion' in which an inversion breakpoint is genotyped by performing fusion PCR on single molecules of human genomic DNA.
Turner, D J, Shendure, J, Porreca, G, Church, G, Green, P, Tyler-Smith, C, Hurles, M E   +6 more
openaire   +3 more sources

Characterization of human aquaporin protein-protein interactions using microscale thermophoresis (MST)

STAR Protocols, 2022
Summary: Aquaporin water channels (AQPs) are membrane proteins that maintain cellular water homeostasis. The interactions between human AQPs and other proteins play crucial roles in AQP regulation by both gating and trafficking.
Tamim Al-Jubair, Jonas Hyld Steffen, Julie Winkel Missel, Philip Kitchen, Mootaz M. Salman, Roslyn M. Bill, Pontus Gourdon, Susanna Törnroth-Horsefield   +7 more
doaj   +1 more source

DNA fiber combing protocol using in-house reagents and coverslips to analyze replication fork dynamics in mammalian cells

STAR Protocols, 2022
Summary: DNA fiber combing is a versatile technique that provides insight into replication fork dynamics at single-molecule resolution. DNA fibers are bound to silanized coverslips and combed, which straightens and aligns the fibers along a single axis ...
Gemma Moore, Judit Jimenez Sainz, Ryan B. Jensen   +2 more
doaj   +1 more source

Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup

STAR Protocols, 2022
Summary: Aquaporins (AQPs) are water channels embedded in the cell membrane that are critical in maintaining water homeostasis. We describe a protocol for determining the water permeation capacity of AQPs reconstituted into proteoliposomes.
Jonas Hyld Steffen, Julie Winkel Missel, Tamim Al-Jubair, Philip Kitchen, Mootaz M. Salman, Roslyn M. Bill, Susanna Törnroth-Horsefield, Pontus Gourdon   +7 more
doaj   +1 more source

A single-molecule assay for telomerase structure-function analysis [PDF]

Nucleic Acids Research, 2009
The activity of the telomerase ribonucleoprotein enzyme is essential for the maintenance of genome stability and normal cell development. Despite the biomedical importance of telomerase activity, detailed structural models for the enzyme remain to be established.
Wu, John Yanyun, Stone, Michael D., Zhuang, Xiaowei   +2 more
openaire   +4 more sources

Two-parameter single-molecule analysis for measurement of chromatin mobility

STAR Protocols, 2020
Summary: This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to
Jonathan Lerner, Pablo Aurelio Gómez-García, Ryan L. McCarthy, Zhe Liu, Melike Lakadamyali, Kenneth S. Zaret   +5 more
doaj   +1 more source

Single-molecule tracking for studying protein dynamics and target-search mechanism in live cells of S. cerevisiae

STAR Protocols, 2022
Summary: Single-molecule tracking (SMT) is a powerful approach to quantify the biophysical parameters of protein dynamics in live cells. Here, we describe a protocol for SMT in live cells of the budding yeast Saccharomyces cerevisiae.
Nitesh Kumar Podh, Ayan Das, Partha Dey, Sheetal Paliwal, Gunjan Mehta   +4 more
doaj   +1 more source

Nanopatterning at the Service of Single Molecule Assays [PDF]

Biophysical Journal, 2010
Today, the manipulation and integration of objects with nanometric dimensions is essential for a great number of applications. In biology and medicine, the study of structural dynamics in individual molecules or other key cellular processes is often limited by the low throughput of current methods.
Cerf, Aline, Vieu, Christophe
openaire   +1 more source

Quantifying the global binding and target-search dynamics of epigenetic regulatory factors using live-cell single-molecule tracking

STAR Protocols, 2021
Summary: This protocol provides instructions to track the global dynamics of single epigenetic regulatory factors in live cells. We describe an approach to generate cell lines that stably express HaloTag-fused proteins.
Kyle Brown, Samantha Kent, Xiaojun Ren
doaj   +1 more source

Single molecule probing of disordered RNA binding proteins

STAR Protocols, 2022
Summary: Liquid-liquid phase separation of intrinsically disordered proteins is known to underlie diverse pathologies such as neurodegeneration, cancer, and aging.
Kevin Rhine, Sua Myong
doaj   +1 more source