Results 41 to 50 of about 70,987 (292)
eLife, 2018 Multi-subunit ring-shaped ATPases are molecular motors that harness chemical free energy to perform vital mechanical tasks such as polypeptide translocation, DNA unwinding, and chromosome segregation.
Ninning Liu +3 more
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A guide to small fluorescent probes for single-molecule biophysics
Chemical Physics Reviews, 2023 The explosive growth of single-molecule techniques is transforming our understanding of biology, helping to develop new physics inspired by emergent biological processes, and leading to emerging areas of nanotechnology. Key biological and chemical processes can now be probed with new levels of detail, one molecule at a time, from the nanoscopic ...
Leake, Mark Christian, Quinn, Steven
openaire +2 more sources
Two-subunit DNA escort mechanism and inactive subunit bypass in an ultra-fast ring ATPase
eLife, 2015 SpoIIIE is a homo-hexameric dsDNA translocase responsible for completing chromosome segregation in Bacillus subtilis. Here, we use a single-molecule approach to monitor SpoIIIE translocation when challenged with neutral-backbone DNA and non-hydrolyzable ...
Ninning Liu +2 more
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Focus on function: Single molecule RNA enzymology [PDF]
, 2007 The ability of RNA to catalyze chemical reactions was first demonstrated 25 years ago with the discovery that group I introns and RNase P function as RNA enzymes (ribozymes).
Mark A. Ditzler +7 more
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Local-to-global signal transduction at the core of a Mn2+ sensing riboswitch
Nature Communications, 2019 Riboswitches bind intracellular metabolites and control bacterial gene expression. Here, by using X-ray crystallography, molecular dynamics simulations, and single-molecule fluorescence resonance energy transfer, the authors show how a local Mn2+ ion ...
Krishna C. Suddala +8 more
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Distribution bias and biochemical characterization of TOP1MT single nucleotide variants
Scientific Reports, 2017 Mitochondrial topoisomerase I (TOP1MT) is a type IB topoisomerase encoded in the nucleus of vertebrate cells. In contrast to the other five human topoisomerases, TOP1MT possesses two high frequency single nucleotide variants (SNVs), rs11544484 (V256I ...
Hongliang Zhang +4 more
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Homology sensing via non-linear amplification of sequence-dependent pausing by RecQ helicase
eLife, 2019 RecQ helicases promote genomic stability through their unique ability to suppress illegitimate recombination and resolve recombination intermediates. These DNA structure-specific activities of RecQ helicases are mediated by the helicase-and-RNAseD like C-
Yeonee Seol +3 more
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Identification of binding mechanisms in single molecule-DNA complexes
, 2003 Eckel R, Ros R, Ros A, Wilking SD, Sewald N, Anselmetti D. Identification of binding mechanisms in single molecule-DNA complexes. Biophysical journal. 2003;85(3):1968-1973.Changes in the elastic properties of single deoxyribonucleic acid (DNA) molecules ...
Anselmetti, Dario +14 more
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Single-molecule conductance of double-stranded RNA oligonucleotides
, 2021 RNA oligonucleotides are crucial for a range of biological functions and in many biotechnological applications. Herein, we measured, for the first time, the conductance of individual double-stranded (ds)RNA molecules and compared it with the conductance ...
Evgenii, Kliuchnikov +6 more
core +1 more source
Transient pauses of the bacterial flagellar motor at low load
New Journal of Physics, 2016 The bacterial flagellar motor (BFM) is the molecular machine responsible for the swimming and chemotaxis of many species of motile bacteria. The BFM is bidirectional, and changes in the rotation direction of the motor are essential for chemotaxis. It has
A L Nord, F Pedaci, R M Berry
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