Results 51 to 60 of about 70,987 (292)

Complete dissection of transcription elongation reveals slow translocation of RNA polymerase II in a linear ratchet mechanism

eLife, 2013
During transcription elongation, RNA polymerase has been assumed to attain equilibrium between pre- and post-translocated states rapidly relative to the subsequent catalysis.
Manchuta Dangkulwanich, Toyotaka Ishibashi, Shixin Liu, Maria L Kireeva, Lucyna Lubkowska, Mikhail Kashlev, Carlos J Bustamante   +6 more
doaj   +1 more source

Single molecule force spectroscopy on ligand-DNA complexes: from molecular binding mechanisms to biosensor applications

, 2004
Ros R, Eckel R, Bartels FW, et al. Single molecule force spectroscopy on ligand-DNA complexes: from molecular binding mechanisms to biosensor applications. Journal of Biotechnology.
Baumgarth, Birgit, Wilking, Sven David, Ros, Robert, Bartels, Frank Wilco, Sischka, Andy, Eckel, Rainer, Pühler, Alfred ; https://orcid.org/, Sewald, Norbert ; https://orcid.org/, Becker, Anke, Anselmetti, Dario ; https://orcid.org/   +9 more
core   +1 more source

High-Speed Single-Molecule Tracking of CXCL13 in the B-Follicle

Frontiers in Immunology, 2018
Soluble factors are an essential means of communication between cells and their environment. However, many molecules readily interact with extracellular matrix components, giving rise to multiple modes of diffusion.
Helen Miller, Helen Miller, Jason Cosgrove, Jason Cosgrove, Jason Cosgrove, Adam J. M. Wollman, Adam J. M. Wollman, Emily Taylor, Emily Taylor, Zhaokun Zhou, Zhaokun Zhou, Peter J. O’Toole, Peter J. O’Toole, Mark C. Coles, Mark C. Coles, Mark C. Coles, Mark C. Leake, Mark C. Leake   +17 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

DeepFRET, a software for rapid and automated single-molecule FRET data classification using deep learning

eLife, 2020
Single-molecule Förster Resonance energy transfer (smFRET) is an adaptable method for studying the structure and dynamics of biomolecules. The development of high throughput methodologies and the growth of commercial instrumentation have outpaced the ...
Johannes Thomsen, Magnus Berg Sletfjerding, Simon Bo Jensen, Stefano Stella, Bijoya Paul, Mette Galsgaard Malle, Guillermo Montoya, Troels Christian Petersen, Nikos S Hatzakis   +8 more
doaj   +1 more source

Single-molecule Biophysics: Machine Learning for Automated Data Processing [PDF]

, 2020
-Single-molecule fluorescence microscopy has been greatly successful in understanding biophysics at molecular level. This technique has been advancing toward higher throughput, which creates a need for a data-analysis tool to distinguish molecule of ...
Soomin Cho, Junhong Choi
core  

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Binding dynamics of a monomeric SSB protein to DNA : a single-molecule multi-process approach

, 2015
People Programme of the European Union’s Seventh Framework Programme [REA 334496 to B.E.B.]; Leonardo da Vinci European Union Programme (to M.F.G.); Wellcome Trust [099149/Z/12/Z, 091825/Z/10/Z]. Funding for open access charge: Wellcome Trust; University
J. Carlos Penedo, Maria Figueira-gonzalez, White, Malcolm F, Ackermann, Katrin, Katrin Ackermann, Penedo, Carlos, Peregrina, Jose Ramon, Bode, Bela Ernest, Michael J. Morten, Figueira-Gonzalez, Maria, Malcolm F. White, Bela E. Bode, Morten, Michael John, Jose R. Peregrina   +13 more
core   +1 more source

Molecular structure, DNA binding mode, photophysical properties and recommendations for use of SYBR Gold [PDF]

, 2021
SYBR Gold is a commonly used and particularly bright fluorescent DNA stain, however, its chemical structure is unknown and its binding mode to DNA remains controversial.
Kolbeck, Pauline J, Lipfert, Jan, Vanderlinden, Willem, Sub Molecular Biophysics, Kolbeck, Pauline J., Gemmecker, Gerd, Gebhardt, Christian, Lak, Aidin, Soft Condensed Matter and Biophysics, Lehmann, Martin, Nicolaus, Thomas, Cordes, Thorben   +11 more
core   +1 more source

Developing in vivo biophysics by fishing for single molecules

Developmental Biology, 2010
Single-molecule techniques (SMT) provide the possibility to quantitatively analyze the action of single molecules. SMTs can resolve the distribution of states of an ensemble of molecules, collecting information that is otherwise not accessible by typical ensemble techniques.
Wang, Xi, Wohland, Thorsten, Korzh, Vladimir   +2 more
openaire   +2 more sources