Results 51 to 60 of about 69,820 (250)
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Phosphoinositides and inositol phosphates as molecular glues
FEBS Letters, EarlyView.Inositol phosphates (IPs) and phosphoinositides (PIPs) regulate diverse eukaryotic processes. Beyond recruiting signaling proteins or acting as structural cofactors, recent studies suggest they mediate protein–protein interactions as natural molecular glues.
Aleshia Seaton‐Terry +9 more
wiley +1 more source
Nucleic Acid Thermodynamics Derived from Mechanical Unzipping Experiments
Life, 2022 Force-spectroscopy techniques have led to significant progress in studying the physicochemical properties of biomolecules that are not accessible in bulk assays. The application of piconewton forces with laser optical tweezers to single nucleic acids has
Paolo Rissone, Felix Ritort
doaj +1 more source
Making connections — strategies for single molecule fluorescence biophysics
Current Opinion in Chemical Biology, 2013 Fluorescence spectroscopy and fluorescence microscopy carried out on the single molecule level are elegant methods to decipher complex biological systems; it can provide a wealth of information that frequently is obscured in the averaging of ensemble measurements.
Grohmann, D, Werner, F, Tinnefeld, P
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New tools to study biophysical properties of single molecules and single cells [PDF]
Anais da Academia Brasileira de Ciências, 2007 We present a review on two new tools to study biophysical properties of single molecules and single cells. A laser incident through a high numerical aperture microscope objective can trap small dielectric particles near the focus. This arrangement is named optical tweezers.
Rocha, Márcio S., Mesquita, Oscar N.
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Modelling stem cell differentiation related processes—A practical overview for biologists
FEBS Letters, EarlyView.Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar +4 more
wiley +1 more source
Nature CommunicationsProtein conformational dynamics are fundamental to enzyme function, yet the molecular mechanisms by which these dynamics are regulated remain poorly understood. Here, we reveal that a conserved network of salt-bridges, modulated by magnesium ions, serves
Yeonee Seol +2 more
doaj +1 more source
Systems biophysics: Single-molecule optical proteomics in single living cells [PDF]
Current Opinion in Systems Biology, 2018 Abstract How does the interplay between biomolecules result in the emergence of cellular complexity at higher length scales? This interplay in even simple biological processes is often too challenging to probe using traditional experimental tools of ensemble averaging across several thousands of molecules.
Shashkova, Sviatlana +1 more
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Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Single-molecule techniques in biophysics: a review of the progress in methods and applications [PDF]
Reports on Progress in Physics, 2017 Abstract Single-molecule biophysics has transformed our understanding of biology, but also of the physics of life. More exotic than simple soft matter, biomatter lives far from thermal equilibrium, covering multiple lengths from the nanoscale of single molecules to up to several orders of magnitude higher in cells,
Miller, Helen Louise +4 more
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