Results 1 to 10 of about 6,472,690 (313)

The Shine-Dalgarno sequence of riboswitch-regulated single mRNAs shows ligand-dependent accessibility bursts

Nature Communications, 2016
In response to intracellular signals, bacterial translational riboswitches embedded in mRNAs can regulate gene expression through inhibition of translation initiation.
Arlie J. Rinaldi, Paul E. Lund, Mario R. Blanco, Nils G. Walter   +3 more
doaj   +1 more source

Resolving Subcellular miRNA Trafficking and Turnover at Single-Molecule Resolution

Cell Reports, 2017
Summary: Regulation of microRNA (miRNA) localization and stability is critical for their extensive cytoplasmic RNA silencing activity and emerging nuclear functions.
Sethuramasundaram Pitchiaya, Laurie A. Heinicke, Jun I. Park, Elizabeth L. Cameron, Nils G. Walter   +4 more
doaj   +1 more source

CRISPR/Cas12a-mediated labeling of MET receptor enables quantitative single-molecule imaging of endogenous protein organization and dynamics

iScience, 2021
Summary: Single-molecule localization microscopy (SMLM) reports on protein organization in cells with near-molecular resolution and in combination with stoichiometric labeling enables protein counting.
Tim N. Baldering, Christos Karathanasis, Marie-Lena I.E. Harwardt, Petra Freund, Matthias Meurer, Johanna V. Rahm, Michael Knop, Marina S. Dietz, Mike Heilemann   +8 more
doaj   +1 more source

An intermolecular FRET sensor detects the dynamics of T cell receptor clustering

Nature Communications, 2017
Cellular signalling is often facilitated by membrane protein clustering, but detection of protein clustering at high spatiotemporal resolution is challenging.
Yuanqing Ma, Elvis Pandzic, Philip R. Nicovich, Yui Yamamoto, Joanna Kwiatek, Sophie V. Pageon, Aleš Benda, Jérémie Rossy, Katharina Gaus   +8 more
doaj   +1 more source

Single-molecule detection on a portable 3D-printed microscope

Nature Communications, 2019
Single-molecule in vitro assays require dedicated confocal microscopes equipped with fluorescence correlation spectroscopy (FCS) modules. Here the authors present a compact, cheap and open-source 3D-printed confocal microscope for single photon counting ...
James W. P. Brown, Arnaud Bauer, Mark E Polinkovsky, Akshay Bhumkar, Dominic J. B. Hunter, Katharina Gaus, Emma Sierecki, Yann Gambin   +7 more
doaj   +1 more source

Protocol to generate fast-dissociating recombinant antibody fragments for multiplexed super-resolution microscopy

STAR Protocols, 2023
Summary: Multiplexed high-density label super-resolution microscopy image reconstruction by integrating exchangeable single-molecule localization (IRIS) enables elucidating fine structures and molecular distribution in cells and tissues.
Qianli Zhang, Akitoshi Miyamoto, Naoki Watanabe   +2 more
doaj   +1 more source

Decoding the Structural Dynamics and Conformational Alternations of DNA Secondary Structures by Single-Molecule FRET Microspectroscopy

Frontiers in Molecular Biosciences, 2021
In addition to the canonical double helix form, DNA is known to be extrapolated into several other secondary structural patterns involving themselves in inter- and intramolecular type hydrogen bonding. The secondary structures of nucleic acids go through
Debolina Bandyopadhyay, Debolina Bandyopadhyay, Padmaja P. Mishra, Padmaja P. Mishra   +3 more
doaj   +1 more source

Live cell imaging of single RNA molecules with fluorogenic Mango II arrays

Nature Communications, 2020
Fluorogenic RNA aptamers have been used for RNA imaging, but folding and fluorescence stability often limited their use in high resolution applications.
Adam D. Cawte, Peter J. Unrau, David S. Rueda   +2 more
doaj   +1 more source

Sphingomyelin metabolism underlies Ras excitability for efficient cell migration and chemotaxis

Cell Structure and Function, 2023
In eukaryotic motile cells, the active Ras (Ras-GTP)-enriched domain is generated in an asymmetric manner on the cell membrane through the excitable dynamics of an intracellular signaling network.
Da Young Shin, Hiroaki Takagi, Michio Hiroshima, Satomi Matsuoka, Masahiro Ueda   +4 more
doaj   +1 more source

The role of urinary pteridines as disease biomarkers

Pteridines, 2017
Pteridines and their derivatives function as intermediates in the metabolism of several vitamins and cofactors, and their relevance to disease has inspired new efforts to study their roles as disease biomarkers.
Burton Casey, Ma Yinfa
doaj   +1 more source