Results 21 to 30 of about 1,085,849 (268)
Single‐molecule genomics [PDF]
The Journal of Pathology, 2009 AbstractThe term ‘single‐molecule genomics’ (SMG) describes a group of molecular methods in which single molecules are detected or sequenced. The focus on the analysis of individual molecules distinguishes these techniques from more traditional methods, in which template DNA is cloned or PCR‐amplified prior to analysis. Although technically challenging,
Frank, McCaughan, Paul H, Dear
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An intermolecular FRET sensor detects the dynamics of T cell receptor clustering
Nature Communications, 2017 Cellular signalling is often facilitated by membrane protein clustering, but detection of protein clustering at high spatiotemporal resolution is challenging.
Yuanqing Ma +8 more
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Resolving Subcellular miRNA Trafficking and Turnover at Single-Molecule Resolution
Cell Reports, 2017 Summary: Regulation of microRNA (miRNA) localization and stability is critical for their extensive cytoplasmic RNA silencing activity and emerging nuclear functions.
Sethuramasundaram Pitchiaya +4 more
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iScience, 2021 Summary: Single-molecule localization microscopy (SMLM) reports on protein organization in cells with near-molecular resolution and in combination with stoichiometric labeling enables protein counting.
Tim N. Baldering +8 more
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Single-molecule detection on a portable 3D-printed microscope
Nature Communications, 2019 Single-molecule in vitro assays require dedicated confocal microscopes equipped with fluorescence correlation spectroscopy (FCS) modules. Here the authors present a compact, cheap and open-source 3D-printed confocal microscope for single photon counting ...
James W. P. Brown +7 more
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Pulling on single molecules [PDF]
Nature Methods, 2012 Single-molecule methods to apply and measure force and displacement are expanding mechanobiology.
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STAR Protocols, 2023 Summary: Multiplexed high-density label super-resolution microscopy image reconstruction by integrating exchangeable single-molecule localization (IRIS) enables elucidating fine structures and molecular distribution in cells and tissues.
Qianli Zhang +2 more
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Frontiers in Molecular Biosciences, 2021 In addition to the canonical double helix form, DNA is known to be extrapolated into several other secondary structural patterns involving themselves in inter- and intramolecular type hydrogen bonding. The secondary structures of nucleic acids go through
Debolina Bandyopadhyay +3 more
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Single-Molecule Reaction-Diffusion
Biophysical Journal, 2023 Abstract We propose to capture reaction-diffusion on a molecule-by-molecule basis from the fastest acquirable timescale, namely individual photon arrivals. We illustrate our method on intrinsically disordered human proteins, the linker histone H1.0 as well as its chaperone prothymosin α
Lance W.Q. Xu +3 more
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Proceedings of the National Academy of Sciences, 2005 We have designed and synthesized a molecular rod that consists of two weakly coupled electronic π -systems with mutually shifted energy levels. The asymmetry thus implied manifests itself in a current–voltage characteristic with pronounced dependence on the sign of the bias voltage, which makes the molecule a prototype for a molecular diode.
Elbing, M. +9 more
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