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Single‐molecule genomics [PDF]
The Journal of Pathology, 2009 AbstractThe term ‘single‐molecule genomics’ (SMG) describes a group of molecular methods in which single molecules are detected or sequenced. The focus on the analysis of individual molecules distinguishes these techniques from more traditional methods, in which template DNA is cloned or PCR‐amplified prior to analysis. Although technically challenging,
Frank, McCaughan, Paul H, Dear
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Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2
Nature Communications, 2022 Single-molecule FRET measurements provide detailed insights into the conformational states and dynamics of human Argonaute 2 that are required for its function at the core of the eukaryotic RNA silencing pathway.
Sarah Willkomm +6 more
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BMC Biology, 2018 Background Higher-order self-assembly of proteins, or “prion-like” polymerisation, is now emerging as a simple and robust mechanism for signal amplification, in particular within the innate immune system, where the recognition of pathogens or danger ...
Ailís O’Carroll +11 more
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Single-molecule nano-optics at low temperature [PDF]
, 2006 The work presented in this thesis involves experiments on single molecules at low temperature. At low temperature, many disturbing temperature activated processes are frozen out, resulting in extremely sharp zero-phonon lines in the fluorescence ex ...
Butter, Jacqueline Yvonne Paula
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Spontaneous Confinement of mRNA Molecules at Biomolecular Condensate Boundaries
Cells, 2023 Cellular biomolecular condensates, termed ribonucleoprotein (RNP) granules, are often enriched in messenger RNA (mRNA) molecules relative to the surrounding cytoplasm.
Rebecca T. Perelman +3 more
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Clinical Chemistry, 2011 The need for assay methods of sufficient sensitivity to determine the low concentrations of hormones present in body fluids led to the original development of immunoassays and analogous “binding” (or “ligand”) assays in the late 1950s and early 1960s. These methods depend on the use of a binding agent (also commonly referred to by other terms such as ...
Roger, Ekins, David, Kelso
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Force transmission by retrograde actin flow-induced dynamic molecular stretching of Talin
Nature Communications, 2023 Force transmission at integrin-based adhesions is important for cell migration and mechanosensing. Talin is an essential focal adhesion (FA) protein that links F-actin to integrins.
Sawako Yamashiro +5 more
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Single Molecule Nanometronome [PDF]
Nano Letters, 2006 We constructed a DNA-based nanomechanical device called the nanometronome. Our device is made by introducing complementary single-stranded overhangs at the two arms of the DNA four-way junction. The ticking rates of this stochastic metronome depend on ion concentrations and can be changed by a set of DNA-based switches to deactivate/reactivate the ...
Chittanon, Buranachai +2 more
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Identification of binding mechanisms in single molecule-DNA complexes [PDF]
, 2003 Eckel R, Ros R, Ros A, Wilking SD, Sewald N, Anselmetti D. Identification of binding mechanisms in single molecule-DNA complexes. Biophysical journal. 2003;85(3):1968-1973.Changes in the elastic properties of single deoxyribonucleic acid (DNA) molecules ...
Anselmetti, Dario +14 more
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Single-molecule chemical denaturation of riboswitches [PDF]
, 2013 To date, single-molecule RNA science has been developed almost exclusively around the effect of metal ions as folding promoters and stabilizers of the RNA structure.
St-Pierre, Patrick +14 more
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