Results 61 to 70 of about 71,811 (209)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

A protocol for single molecule imaging and tracking of processive myosin motors

MethodsX, 2019
Myosin is a large family of actin-based molecular motors, which includes efficient intracellular transporters that move cargoes and material essential for cell’s life.
Lucia Gardini, Claudia Arbore, Marco Capitanio, Francesco Saverio Pavone   +3 more
doaj   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Real-Time Multistep Asymmetrical Disassembly of Nucleosomes and Chromatosomes Visualized by High-Speed Atomic Force Microscopy

ACS Central Science, 2023
Bibiana Onoa, César Díaz-Celis, Cristhian Cañari-Chumpitaz, Antony Lee, Carlos Bustamante   +4 more
doaj   +1 more source

Protocol to quantify mechanical properties of cells using optical tweezers

STAR Protocols
Summary: Mechanical properties of cells determine their ability to deform when subjected to force, which is crucial in many biological processes. Here, we present a protocol to quantify these properties on living cells by applying piconewton-range forces
Wessel S. Rodenburg, Jorine M. Eeftens
doaj   +1 more source

CTCF and cohesin regulate chromatin loop stability with distinct dynamics

eLife, 2017
Folding of mammalian genomes into spatial domains is critical for gene regulation. The insulator protein CTCF and cohesin control domain location by folding domains into loop structures, which are widely thought to be stable.
Anders S Hansen, Iryna Pustova, Claudia Cattoglio, Robert Tjian, Xavier Darzacq   +4 more
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Single-Molecule Tracking in Live Cell without Immobilization or without Hydrodynamic Flow by Simulations: Thermodynamic Jitter

Biophysica
Experiments to measure a single molecule/particle, i.e., an individual molecule/particle, at room temperature or under physiological conditions without immobilization—for example, on a surface or without significant hydrodynamic flow—have so far failed ...
Gerd Baumann, Zeno Földes-Papp
doaj   +1 more source

Optical tweezers studies of transcription by eukaryotic RNA polymerases

Biomolecular Concepts, 2017
Transcription is the first step in the expression of genetic information and it is carried out by large macromolecular enzymes called RNA polymerases.
Lisica Ana, Grill Stephan W.
doaj   +1 more source

The light side of the force

eLife, 2016
A combination of two single-molecule techniques has revealed new tertiary interactions in the TPP riboswitch.
Aakash Basu, Taekjip Ha
doaj   +1 more source