Results 1 to 10 of about 10,630 (246)
High spatiotemporal resolution data from a custom magnetic tweezers instrument [PDF]
Data in Brief, 2020 Gene expression is achieved by enzymes as RNA polymerases that translocate along nucleic acids with steps as small as a single base pair, i.e., 0.34 nm for DNA.
Eugeniu Ostrofet +2 more
doaj +4 more sources
Magnetic tweezers: development and use in single-molecule research [PDF]
BioTechniques, 2022 The use of magnetic tweezers for single-molecule micromanipulation has evolved rapidly since its introduction approximately 30 years ago. Magnetic tweezers have provided important insights into the dynamic activity of DNA-processing enzymes, as well as ...
Santosh Gaire +3 more
doaj +4 more sources
A guide to magnetic tweezers and their applications [PDF]
Frontiers in Physics, 2016 Magnetic force spectroscopy is a rapidly developing single molecule technique that found numerous applications at the interface of physics and biology. Since the invention of the first magnetic tweezers, a number of modifications were incorporated into ...
Rupa Sarkar, Valentin V Rybenkov
doaj +4 more sources
Magnetic forces and DNA mechanics in multiplexed magnetic tweezers. [PDF]
PLoS ONE, 2012 Magnetic tweezers (MT) are a powerful tool for the study of DNA-enzyme interactions. Both the magnet-based manipulation and the camera-based detection used in MT are well suited for multiplexed measurements.
Iwijn De Vlaminck +4 more
doaj +8 more sources
Correction-free force calibration for magnetic tweezers experiments [PDF]
Scientific Reports, 2018 Magnetic tweezers are a powerful technique to perform high-throughput and high-resolution force spectroscopy experiments at the single-molecule level. The camera-based detection of magnetic tweezers enables the observation of hundreds of magnetic beads ...
David Dulin, Dulin David
exaly +4 more sources
A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins [PDF]
Molecules, 2021 We report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers.
Roberto Fabian +5 more
doaj +2 more sources
Direct Measurement of the Stall Torque of the Flagellar Motor in Escherichia coli with Magnetic Tweezers [PDF]
mBio, 2022 The flagellar motor drives the rotation of flagellar filaments, propelling the swimming of flagellated bacteria. The maximum torque the motor generates, the stall torque, is a key characteristic of the motor function.
Bin Wang +3 more
doaj +2 more sources
Magnetic Tweezers in a Microplate Format [PDF]
Journal of Visualized Experiments, 2022 Mechanobiology describes how the physical forces and mechanical properties of biological material contribute to physiology and disease. Typically, these approaches are limited single-molecule methods, which restricts their availability. To address this need, a microplate assay was developed that enables mechanical manipulation while performing standard
dos Santos, A., Toseland, C.
openaire +5 more sources
An Introduction to Magnetic Tweezers [PDF]
, 2023 AbstractMagnetic tweezers are a single-molecule force and torque spectroscopy technique that enable the mechanical interrogation in vitro of biomolecules, such as nucleic acids and proteins. They use a magnetic field originating from either permanent magnets or electromagnets to attract a magnetic particle, thus stretching the tethering biomolecule ...
Dulin, David
openaire +3 more sources
Quantitative Modeling and Optimization of Magnetic Tweezers [PDF]
Biophysical Journal, 2009 Magnetic tweezers are a powerful tool to manipulate single DNA or RNA molecules and to study nucleic acid-protein interactions in real time. Here, we have modeled the magnetic fields of permanent magnets in magnetic tweezers and computed the forces exerted on superparamagnetic beads from first principles.
Lipfert, Jan +2 more
openaire +4 more sources