High spatiotemporal resolution data from a custom magnetic tweezers instrument [PDF]
Data in Brief, 2020 Gene expression is achieved by enzymes as RNA polymerases that translocate along nucleic acids with steps as small as a single base pair, i.e., 0.34 nm for DNA.
Eugeniu Ostrofet +2 more
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Magnetic tweezers: development and use in single-molecule research
BioTechniques, 2022 The use of magnetic tweezers for single-molecule micromanipulation has evolved rapidly since its introduction approximately 30 years ago. Magnetic tweezers have provided important insights into the dynamic activity of DNA-processing enzymes, as well as ...
Santosh Gaire +3 more
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A guide to magnetic tweezers and their applications [PDF]
Frontiers in Physics, 2016 Magnetic force spectroscopy is a rapidly developing single molecule technique that found numerous applications at the interface of physics and biology. Since the invention of the first magnetic tweezers, a number of modifications were incorporated into ...
Rupa Sarkar, Valentin V Rybenkov
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Magnetic forces and DNA mechanics in multiplexed magnetic tweezers. [PDF]
PLoS ONE, 2012 Magnetic tweezers (MT) are a powerful tool for the study of DNA-enzyme interactions. Both the magnet-based manipulation and the camera-based detection used in MT are well suited for multiplexed measurements.
Iwijn De Vlaminck +4 more
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A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins [PDF]
Molecules, 2021 We report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers.
Roberto Fabian +5 more
doaj +2 more sources
Direct Measurement of the Stall Torque of the Flagellar Motor in Escherichia coli with Magnetic Tweezers [PDF]
mBio, 2022 The flagellar motor drives the rotation of flagellar filaments, propelling the swimming of flagellated bacteria. The maximum torque the motor generates, the stall torque, is a key characteristic of the motor function.
Bin Wang +3 more
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Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision [PDF]
Nature Communications, 2020 Single molecule force measurements have shed light on dynamic biological events, but rare events escape notice owing to low throughput of the methods. Here, the authors combine an array of magnetic tweezers with lateral flow to increase throughput 100 ...
Rohit Agarwal, Karl E. Duderstadt
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Correlating fluorescence microscopy, optical and magnetic tweezers to study single chiral biopolymers such as DNA [PDF]
Nature CommunicationsBiopolymer topology is critical for determining interactions inside cell environments, exemplified by DNA where its response to mechanical perturbation is as important as biochemical properties to its cellular roles.
Jack W. Shepherd +9 more
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A Horizontal Magnetic Tweezers and Its Use for Studying Single DNA Molecules [PDF]
Micromachines, 2018 We report the development of a magnetic tweezers that can be used to micromanipulate single DNA molecules by applying picoNewton (pN)-scale forces in the horizontal plane. The resulting force–extension data from our experiments show high-resolution
Roberto Fabian +4 more
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Magnetic tweezers for the measurement of twist and torque. [PDF]
J Vis Exp, 2014 Single-molecule techniques make it possible to investigate the behavior of individual biological molecules in solution in real time. These techniques include so-called force spectroscopy approaches such as atomic force microscopy, optical tweezers, flow stretching, and magnetic tweezers.
Lipfert J +4 more
europepmc +5 more sources