Magnetic tweezers to capture the fast-folding λ6-85 in slow motion [PDF]
Communications PhysicsForce spectroscopy gives access to the underlying free energy landscape of protein folding. Proteins exhibit folding rates between microseconds and hours.
César Augusto Quintana-Cataño +5 more
doaj +3 more sources
Correction-free force calibration for magnetic tweezers experiments [PDF]
Scientific Reports, 2018 Magnetic tweezers are a powerful technique to perform high-throughput and high-resolution force spectroscopy experiments at the single-molecule level. The camera-based detection of magnetic tweezers enables the observation of hundreds of magnetic beads ...
David Dulin
exaly +3 more sources
Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision [PDF]
Nature Communications, 2020 Single molecule force measurements have shed light on dynamic biological events, but rare events escape notice owing to low throughput of the methods. Here, the authors combine an array of magnetic tweezers with lateral flow to increase throughput 100 ...
Rohit Agarwal, Karl E. Duderstadt
doaj +2 more sources
Quantitative Modeling and Optimization of Magnetic Tweezers [PDF]
Biophysical Journal, 2009 Magnetic tweezers are a powerful tool to manipulate single DNA or RNA molecules and to study nucleic acid-protein interactions in real time. Here, we have modeled the magnetic fields of permanent magnets in magnetic tweezers and computed the forces exerted on superparamagnetic beads from first principles.
Lipfert, Jan +2 more
openaire +4 more sources
Magnetic tweezers for the measurement of twist and torque. [PDF]
J Vis Exp, 2014 Single-molecule techniques make it possible to investigate the behavior of individual biological molecules in solution in real time. These techniques include so-called force spectroscopy approaches such as atomic force microscopy, optical tweezers, flow stretching, and magnetic tweezers.
Lipfert J +4 more
europepmc +5 more sources
Skewed brownian fluctuations in single-molecule magnetic tweezers. [PDF]
PLoS ONE, 2014 Measurements in magnetic tweezers rely upon precise determination of the position of a magnetic microsphere. Fluctuations in the position due to Brownian motion allows calculation of the applied force, enabling deduction of the force-extension response ...
Daniel R Burnham +3 more
doaj +2 more sources
Label-free microcargo delivery and controllable release in complex fluidic environments based on autonomous magnetic microswarm [PDF]
Microsystems & NanoengineeringMagnetic microswarms exhibit great potential for targeted delivery because of their excellent controllability and environmental adaptability. Enabling label-free cargo delivery and controllable release while endowing microswarms with resistance to ...
Zhixin Wu +11 more
doaj +2 more sources
Design of an automated cell batch microinjection system based on magnetic tweezers for zebrafish embryos. [PDF]
Microsyst NanoengGuo X +8 more
europepmc +3 more sources
Measurement of the magnetic moment of single Magnetospirillum gryphiswaldense cells by magnetic tweezers [PDF]
Scientific Reports, 2017 Magnetospirillum gryphiswaldense is a helix-shaped magnetotactic bacterium that synthesizes iron-oxide nanocrystals, which allow navigation along the geomagnetic field.
C. Zahn +9 more
doaj +2 more sources
Protein force spectroscopy using magnetic tweezers: Slow and steady wins the race? [PDF]
Biophys JPritzl SD, Lipfert J.
europepmc +2 more sources