Results 41 to 50 of about 9,222 (148)
Topoisomerase VI is a chirally-selective, preferential DNA decatenase
eLife, 2022 DNA topoisomerase VI (topo VI) is a type IIB DNA topoisomerase found predominantly in archaea and some bacteria, but also in plants and algae. Since its discovery, topo VI has been proposed to be a DNA decatenase; however, robust evidence and a mechanism
Shannon J McKie +5 more
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Insights on the effect of macromolecular crowding on transcription and its regulation
QRB DiscoveryTranscription of DNA into RNA is a fundamental cellular process upon which life depends. It is tightly regulated in several different ways, and among the most important mechanisms are protein-induced topological changes in DNA such as looping.
Wenxuan Xu +4 more
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The mechanism of DNA unwinding by the eukaryotic replicative helicase
Nature Communications, 2019 How the eukaryotic helicase unzips DNA during replication is not well understood. By measuring the real-time motion of purified CMG unwinding DNA with magnetic tweezers, the authors reveal the dynamics where isolated CMG unwinds via a biased random walk ...
Daniel R. Burnham +3 more
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Single molecule high-throughput footprinting of small and large DNA ligands
Nature Communications, 2017 Mapping the sequence specificity of DNA ligands remains a challenge, particularly for small drugs. Here the authors develop a parallelized single molecule magnetic tweezers approach using engineered DNA hairpins that can detect sequence selectivity ...
Maria Manosas +3 more
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Microrheology: From Video Microscopy to Optical Tweezers
MicromachinesMicrorheology, a branch of rheology, focuses on studying the flow and deformation of matter at micron length scales, enabling the characterization of materials using minute sample volumes.
Andrea Jannina Fernandez +3 more
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eLife, 2021 Faithful segregation of bacterial chromosomes relies on the ParABS partitioning system and the SMC complex. In this work, we used single-molecule techniques to investigate the role of cytidine triphosphate (CTP) binding and hydrolysis in the critical ...
Francisco de Asis Balaguer +7 more
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Protein Unfolding: Denaturant vs. Force
Biomedicines, 2021 While protein refolding has been studied for over 50 years since the pioneering work of Christian Anfinsen, there have been a limited number of studies correlating results between chemical, thermal, and mechanical unfolding.
Colleen Kelly, Matthew J. Gage
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A force calibration standard for magnetic tweezers
Review of Scientific Instruments, 2014 To study the behavior of biological macromolecules and enzymatic reactions under force, advances in single-molecule force spectroscopy have proven instrumental. Magnetic tweezers form one of the most powerful of these techniques, due to their overall simplicity, non-invasive character, potential for high throughput measurements, and large force range ...
Zhongbo Yu +9 more
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Magnetic Tweezers Measurement of Single Molecule Torque [PDF]
Nano Letters, 2009 Torsional stress in linear biopolymers such as DNA and chromatin has important consequences for nanoscale biological processes. We have developed a new method to directly measure torque on single molecules. Using a cylindrical magnet, we manipulate a novel probe consisting of a nanorod with a 0.1 microm ferromagnetic segment coupled to a magnetic bead.
Celedon, Alfredo +7 more
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Trigger factor chaperone acts as a mechanical foldase
Nature Communications, 2017 Proteins fold under mechanical force during co-translational folding at the ribosome. Here, the authors use single molecule magnetic tweezers to study the influence of chaperones on protein folding and show that the ribosomal chaperone trigger factor ...
Shubhasis Haldar +4 more
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