Results 51 to 60 of about 10,645 (268)
Data in Brief, 2020 Nucleic acids are central to the storage and transmission of genetic information and play essential roles in many cellular processes. Quantitative understanding and modeling of their functions and properties requires quantitative experimental ...
Willem Vanderlinden +4 more
doaj +1 more source
Communications Biology, 2021 Rieu et al. present a magnetic tweezers based single-molecule manipulation method, called kinetic locking, for direct detection of biomolecular binding without use of fluorescent probes. By measuring dynamical interactions of E.
Martin Rieu +4 more
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Magnetic tweezers for intracellular applications [PDF]
Review of Scientific Instruments, 2003 We have designed and constructed a versatile magnetic tweezer primarily for intracellular investigations. The micromanipulator uses only two coils to simultaneously magnetize to saturation micron-size superparamagnetic particles and generate high magnitude constant field gradients over cellular dimensions. The apparatus resembles a miniaturized Faraday
Hosu, Basarab Gabriel, 1968- +4 more
openaire +1 more source
Single-molecule study reveals Hmo1, not Hho1, promotes chromatin assembly in budding yeast
mBio, 2023 Linker histone H1 plays a crucial role in various biological processes, including nucleosome stabilization, high-order chromatin structure organization, gene expression, and epigenetic regulation in eukaryotic cells.
Mengxue Wang +8 more
doaj +1 more source
Robust membrane protein tweezers reveal the folding speed limit of helical membrane proteins
eLife, 2023 Single-molecule tweezers, such as magnetic tweezers, are powerful tools for probing nm-scale structural changes in single membrane proteins under force.
Seoyoon Kim +3 more
doaj +1 more source
Mechanical activation of vinculin binding to talin locks talin in an unfolded conformation [PDF]
, 2014 The force-dependent interaction between talin and vinculin plays a crucial role in the initiation and growth of focal adhesions. Here we use magnetic tweezers to characterise the mechano-sensitive compact N-terminal region of the talin rod, and show that
J. Yan (3377042) +26 more
core +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Drug Targeting Model of Composite Gold-Tourmaline for Cells Enhancing Applications
Nano Biomedicine and Engineering, 2015 The drug delivery and targeting method of gold tourmaline composite atoms generated by optical tweezers in a micro-optical device system is proposed.
Preecha P. Yupapin, Senee Suwandee
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Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1
FEBS Open Bio, EarlyView.The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay +2 more
wiley +1 more source
Magnetic tweezers for manipulation of magnetic particles in single cells
, 2014 Ebrahimian H, Giesguth M, Dietz K-J, Reiss G, Herth S. Magnetic tweezers for manipulation of magnetic particles in single cells. Applied Physics Letters. 2014;104(6): 63701.Magnetic tweezers gain increasing interest for applications in biology.
Dietz, Karl-Josef +4 more
core +1 more source