Results 61 to 70 of about 1,562,446 (293)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Microscopic applications of holographic beam shaping and studies of optically trapped aerosols [PDF]

, 2009
This thesis has two themes. Firstly, it concerns the original application of holographic beam shaping, employed through the methods associated with optical manipulation, to three microscopic fields of research.
Burnham, Daniel R.
core   +2 more sources

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Topoisomerase VI is a chirally-selective, preferential DNA decatenase

eLife, 2022
DNA topoisomerase VI (topo VI) is a type IIB DNA topoisomerase found predominantly in archaea and some bacteria, but also in plants and algae. Since its discovery, topo VI has been proposed to be a DNA decatenase; however, robust evidence and a mechanism
Shannon J McKie, Parth Rakesh Desai, Yeonee Seol, Adam MB Allen, Anthony Maxwell, Keir C Neuman   +5 more
doaj   +1 more source

Magnetic tweezers for intracellular applications [PDF]

Review of Scientific Instruments, 2003
We have designed and constructed a versatile magnetic tweezer primarily for intracellular investigations. The micromanipulator uses only two coils to simultaneously magnetize to saturation micron-size superparamagnetic particles and generate high magnitude constant field gradients over cellular dimensions. The apparatus resembles a miniaturized Faraday
Hosu, Basarab Gabriel, 1968-, Jakab, Karoly Robert, 1974-, Banki, Peter, Toth, Ferenc I., Forgács, Gabor, 1949-   +4 more
openaire   +1 more source

Magnetically Driven Biofabrication for Tissue Engineering: From Nanoparticle Design to Mag‐ATMP Translation

Advanced Healthcare Materials, EarlyView.
Magnetically responsive nanoparticles enable remote control over biomaterials, cells, and engineered tissues. This review summarizes material design, biological safety, fabrication and actuation strategies, computational validation, and translational pathways that support the development of magnetic tissue‐engineering platforms and future magnetically ...
Konstantinos Ioannidis, Dimitrios Lefas, Maya Guilliams, Jackie Van Grootenbril, Isaak Decoene, Dimitrios Sakellariou, Bart Smeets, Stefanos Mourdikoudis, Ioannis Papantoniou   +8 more
wiley   +1 more source

Insights on the effect of macromolecular crowding on transcription and its regulation

QRB Discovery
Transcription of DNA into RNA is a fundamental cellular process upon which life depends. It is tightly regulated in several different ways, and among the most important mechanisms are protein-induced topological changes in DNA such as looping.
Wenxuan Xu, Dylan Collette, Jin Qian, Laura Finzi, David Dunlap   +4 more
doaj   +1 more source

Magnetic Actuation of Smooth Muscle Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles [PDF]

, 2015
Faecal incontinence (FI) is a debilitating disorder that affects a significant portion of the population. The research included in this thesis aimed to test the hypothesis that magnetic actuating of smooth muscle cells loaded with superparamagnetic iron ...
Angelopoulos, I
core  

Optical Detection of Cellular Signals at Material Interfaces

Advanced Healthcare Materials, EarlyView.
Emerging functional materials are transforming optical detection of cellular signals. This review highlights optical techniques that exploit the unique optical properties of diverse materials to detect and quantify cellular electrical, chemical, and mechanical signals, and discusses key opportunities and challenges.
Xuchen Ren, Jitong Ren, Zixin Zhang, Koney Eungkon Kim, Francis L. Hong, Yuecheng Peter Zhou   +5 more
wiley   +1 more source

Microrheology: From Video Microscopy to Optical Tweezers

Micromachines
Microrheology, a branch of rheology, focuses on studying the flow and deformation of matter at micron length scales, enabling the characterization of materials using minute sample volumes.
Andrea Jannina Fernandez, Graham M. Gibson, Anna Rył, Manlio Tassieri   +3 more
doaj   +1 more source