Results 31 to 40 of about 10,587 (256)

Applying torque to the Escherichia coli flagellar motor using magnetic tweezers. [PDF]

Sci Rep, 2017
The bacterial flagellar motor of Escherichia coli is a nanoscale rotary engine essential for bacterial propulsion. Studies on the power output of single motors rely on the measurement of motor torque and rotation under external load. Here, we investigate
van Oene MM, Dickinson LE, Cross B, Pedaci F, Lipfert J, Dekker NH.   +5 more
europepmc   +2 more sources

Micro Magnetic Tweezers for Nanomanipulation Inside Live Cells [PDF]

, 2005
This study reports the design, realization, and characterization of a multi-pole magnetic tweezers that enables us to maneuver small magnetic probes inside living cells.
de Vries, Anthony H.B., de Vries, A.H.B., van Driel, Roel, Kanger, Johannes S., Krenn, Bea E., van Driel, R., Kanger, J.S., Krenn, G.E., de Vries, A.H.   +8 more
core   +1 more source

Developing a New Biophysical Tool to Combine Magneto-Optical Tweezers with Super-Resolution Fluorescence Microscopy

Photonics, 2015
We present a novel experimental setup in which magnetic and optical tweezers are combined for torque and force transduction onto single filamentous molecules in a transverse configuration to allow simultaneous mechanical measurement and manipulation ...
Zhaokun Zhou, Helen Miller, Adam J.M. Wollman, Mark C. Leake   +3 more
doaj   +1 more source

Characterization of G-Quadruplexes Folding/Unfolding Dynamics and Interactions with Proteins from Single-Molecule Force Spectroscopy

Biomolecules, 2021
G-quadruplexes (G4s) are stable secondary nucleic acid structures that play crucial roles in many fundamental biological processes. The folding/unfolding dynamics of G4 structures are associated with the replication and transcription regulation functions
Yuanlei Cheng, Yashuo Zhang, Huijuan You
doaj   +1 more source

Direct observation of chaperone-modulated talin mechanics with single-molecule resolution

Communications Biology, 2022
Chakraborty et al. uses single-molecule magnetic tweezers to investigate the chaperone-modulated talin protein mechanics. The results showed that chaperones are involved in the regulation of talin folding/unfolding under mechanical force with some ...
Soham Chakraborty, Deep Chaudhuri, Souradeep Banerjee, Madhu Bhatt, Shubhasis Haldar   +4 more
doaj   +1 more source

Portable magnetic tweezers device enables visualization of the three-dimensional microscale deformation of soft biological materials

BioTechniques, 2011
We have designed and built a magnetic tweezers device that enables the application of calibrated stresses to soft materials while simultaneously measuring their microscale deformation using confocal microscopy.
Yali Yang, Jun Lin, Ryan Meschewski, Erin Watson, Megan T. Valentine   +4 more
doaj   +1 more source

Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA

Communications Biology, 2021
Wang et al. show how genetic sequence and base modifications can be detected simultaneously on single molecules of both DNA and RNA using magnetic tweezers.
Zhen Wang, Jérôme Maluenda, Laurène Giraut, Thibault Vieille, Andréas Lefevre, David Salthouse, Gaël Radou, Rémi Moulinas, Sandra Astete, Pol D’Avezac, Geoff Smith, Charles André, Jean-François Allemand, David Bensimon, Vincent Croquette, Jimmy Ouellet, Gordon Hamilton   +16 more
doaj   +1 more source

Acoustic Devices for Particle and Cell Manipulation and Sensing [PDF]

, 2014
An emerging demand for the precise manipulation of cells and particles for applications in cell biology and analytical chemistry has driven rapid development of ultrasonic manipulation technology.
Hughes, David, Sylvia Gebhardt, Poltarjonoks, Romans, Sandy Cochran, David A. Hughes, Cochran, Sandy, Aleksandrs Bolhovitins, Gebhardt, Sylvia, Han Wang, Romans Poltarjonoks, Martyn Hill, Schönecker, Andreas, Peter Glynne-Jones, Christine Demore, Christine E. M. Demore, Weijer, Kees; id_orcid, Kees Weijer, Hill, Martyn, Hughes, David A., Glynne-Jones, Peter, Demore, Christine E. M., Bolhovitins, Aleksandrs, Yongqiang Qiu, Demore, Christine, David Hughes, Andreas Schönecker, Qiu, Yongqiang, Wang, Han, Weijer, Kees   +28 more
core   +1 more source

Clinical and Experimental Substantiation of the Possibility of Using a 25G Tip to a Permanent Magnet to Remove Intraocular Foreign Bodies

Oftalʹmologiâ, 2022
Purpose: to develop, experimentally study and evaluate in the clinic the effectiveness of using a 25G handpiece to a permanent operating magnet for IOFB removal.Materials and methods.
A. A. Mikhin, S. V. Churashov, A. N. Kulikov, D. S. Ilyushchenkov   +3 more
doaj   +1 more source

Localized force application reveals mechanically sensitive domains of Piezo1

Nature Communications, 2016
Piezo ion channels transduce mechanical forces into biological signals. Here, the authors use magnetic tweezers to pull specific regions of Piezo1, identifying mechanically sensitive domains that are essential for channel activation and inactivation.
Jason Wu, Raman Goyal, Jörg Grandl
doaj   +1 more source