Results 31 to 40 of about 70,987 (292)
Single-Molecule Cellular Biophysics [PDF]
Journal of Biomedical Optics, 2013 Research on single-molecule cellular biophysics is an emerging field that is rapidly advancing based on the development of new instrumentation and experimental designs. To understand complex biological systems, researchers require instrumentation and techniques that span many orders of scale.
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Systematic analysis of the molecular and biophysical properties of key DNA damage response factors
eLife, 2023 Repair of DNA double strand breaks (DSBs) is integral to preserving genomic integrity. Therefore, defining the mechanisms underlying DSB repair will enhance our understanding of how defects in these pathways contribute to human disease and could lead to ...
Joshua R Heyza +4 more
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Multiple events on single molecules: Unbiased estimation in single-molecule biophysics [PDF]
Proceedings of the National Academy of Sciences, 2006 Most analyses of single-molecule experiments consist of binning experimental outcomes into a histogram and finding the parameters that optimize the fit of this histogram to a given data model. Here we show that such an approach can introduce biases in the estimation of the parameters, thus great care must be taken in the estimation of model parameters ...
Koster, Daniel A. +2 more
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Nature Communications, 2022 Human Bloom’s syndrome (BLM) helicase has a role in DNA repair, and BLM deficiency in humans is associated with chromosomal abnormalities. Here the authors employ solution biophysical assays to show BLM maintains a balance for disruption and ...
Gábor M. Harami +7 more
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Biophysical Reports, 2022 : Single-molecule spectroscopy has revolutionized molecular biophysics and provided means to probe how structural moieties within biomolecules spatially reorganize at different timescales.
Paul David Harris, Eitan Lerner
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Conformational dynamics and allosteric modulation of the SARS-CoV-2 spike
eLife, 2022 Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects cells through binding to angiotensin-converting enzyme 2 (ACE2). This interaction is mediated by the receptor-binding domain (RBD) of the viral spike (S) glycoprotein.
Marco A Díaz-Salinas +7 more
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Properties of Piezoresistive Silicon Nano-scale Cantilevers with Applications to BioNEMS [PDF]
, 2006 Over the last decade a great deal of interest has been raised in applications of Microelectromechanical Sensors [MEMS] for the detection of biological molecules and to the study of their forces of interaction.
Arlett, Jessica Lynn
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Distinct RPA domains promote recruitment and the helicase-nuclease activities of Dna2
Nature Communications, 2021 An enzymatic ensemble including Dna2 functions in DNA end resection; the function of the single-stranded DNA binding protein RPA in this complex has been underappreciated.
Ananya Acharya +6 more
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Protocol for generation and regeneration of PEG-passivated slides for single-molecule measurements
STAR Protocols, 2022 Summary: Single-molecule fluorescence detection by total internal reflection microscope requires surface passivation by polyethylene glycol (PEG) coating, which is labor intensive and is only good for one or two experiments. Here, we present an efficient
Tapas Paul, Sua Myong
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Hierarchical mechanism of amino acid sensing by the T-box riboswitch
Nature Communications, 2018 Riboswitches on 5′ ends of mRNAs are important for bacterial gene regulation. Here the authors probe the mechanism of a tRNA aminoacylation sensing T-box riboswitch using single-molecule fluorescence microscopy to characterize dynamic solution ...
Krishna C. Suddala +5 more
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