Results 31 to 40 of about 69,820 (250)
Distinct RPA domains promote recruitment and the helicase-nuclease activities of Dna2
Nature Communications, 2021 An enzymatic ensemble including Dna2 functions in DNA end resection; the function of the single-stranded DNA binding protein RPA in this complex has been underappreciated.
Ananya Acharya +6 more
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Protocol for generation and regeneration of PEG-passivated slides for single-molecule measurements
STAR Protocols, 2022 Summary: Single-molecule fluorescence detection by total internal reflection microscope requires surface passivation by polyethylene glycol (PEG) coating, which is labor intensive and is only good for one or two experiments. Here, we present an efficient
Tapas Paul, Sua Myong
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Hierarchical mechanism of amino acid sensing by the T-box riboswitch
Nature Communications, 2018 Riboswitches on 5′ ends of mRNAs are important for bacterial gene regulation. Here the authors probe the mechanism of a tRNA aminoacylation sensing T-box riboswitch using single-molecule fluorescence microscopy to characterize dynamic solution ...
Krishna C. Suddala +5 more
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eLife, 2018 Multi-subunit ring-shaped ATPases are molecular motors that harness chemical free energy to perform vital mechanical tasks such as polypeptide translocation, DNA unwinding, and chromosome segregation.
Ninning Liu +3 more
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Two-subunit DNA escort mechanism and inactive subunit bypass in an ultra-fast ring ATPase
eLife, 2015 SpoIIIE is a homo-hexameric dsDNA translocase responsible for completing chromosome segregation in Bacillus subtilis. Here, we use a single-molecule approach to monitor SpoIIIE translocation when challenged with neutral-backbone DNA and non-hydrolyzable ...
Ninning Liu +2 more
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Local-to-global signal transduction at the core of a Mn2+ sensing riboswitch
Nature Communications, 2019 Riboswitches bind intracellular metabolites and control bacterial gene expression. Here, by using X-ray crystallography, molecular dynamics simulations, and single-molecule fluorescence resonance energy transfer, the authors show how a local Mn2+ ion ...
Krishna C. Suddala +8 more
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A guide to small fluorescent probes for single-molecule biophysics
Chemical Physics Reviews, 2023 The explosive growth of single-molecule techniques is transforming our understanding of biology, helping to develop new physics inspired by emergent biological processes, and leading to emerging areas of nanotechnology. Key biological and chemical processes can now be probed with new levels of detail, one molecule at a time, from the nanoscopic ...
Leake, Mark Christian, Quinn, Steven
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Distribution bias and biochemical characterization of TOP1MT single nucleotide variants
Scientific Reports, 2017 Mitochondrial topoisomerase I (TOP1MT) is a type IB topoisomerase encoded in the nucleus of vertebrate cells. In contrast to the other five human topoisomerases, TOP1MT possesses two high frequency single nucleotide variants (SNVs), rs11544484 (V256I ...
Hongliang Zhang +4 more
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Homology sensing via non-linear amplification of sequence-dependent pausing by RecQ helicase
eLife, 2019 RecQ helicases promote genomic stability through their unique ability to suppress illegitimate recombination and resolve recombination intermediates. These DNA structure-specific activities of RecQ helicases are mediated by the helicase-and-RNAseD like C-
Yeonee Seol +3 more
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Transient pauses of the bacterial flagellar motor at low load
New Journal of Physics, 2016 The bacterial flagellar motor (BFM) is the molecular machine responsible for the swimming and chemotaxis of many species of motile bacteria. The BFM is bidirectional, and changes in the rotation direction of the motor are essential for chemotaxis. It has
A L Nord, F Pedaci, R M Berry
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