Results 21 to 30 of about 71,811 (209)
Nature Communications, 2022 Human Bloom’s syndrome (BLM) helicase has a role in DNA repair, and BLM deficiency in humans is associated with chromosomal abnormalities. Here the authors employ solution biophysical assays to show BLM maintains a balance for disruption and ...
Gábor M. Harami +7 more
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Conformational dynamics and allosteric modulation of the SARS-CoV-2 spike
eLife, 2022 Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects cells through binding to angiotensin-converting enzyme 2 (ACE2). This interaction is mediated by the receptor-binding domain (RBD) of the viral spike (S) glycoprotein.
Marco A Díaz-Salinas +7 more
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Biophysical Reports, 2022 : Single-molecule spectroscopy has revolutionized molecular biophysics and provided means to probe how structural moieties within biomolecules spatially reorganize at different timescales.
Paul David Harris, Eitan Lerner
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Distinct RPA domains promote recruitment and the helicase-nuclease activities of Dna2
Nature Communications, 2021 An enzymatic ensemble including Dna2 functions in DNA end resection; the function of the single-stranded DNA binding protein RPA in this complex has been underappreciated.
Ananya Acharya +6 more
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Protocol for generation and regeneration of PEG-passivated slides for single-molecule measurements
STAR Protocols, 2022 Summary: Single-molecule fluorescence detection by total internal reflection microscope requires surface passivation by polyethylene glycol (PEG) coating, which is labor intensive and is only good for one or two experiments. Here, we present an efficient
Tapas Paul, Sua Myong
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Hierarchical mechanism of amino acid sensing by the T-box riboswitch
Nature Communications, 2018 Riboswitches on 5′ ends of mRNAs are important for bacterial gene regulation. Here the authors probe the mechanism of a tRNA aminoacylation sensing T-box riboswitch using single-molecule fluorescence microscopy to characterize dynamic solution ...
Krishna C. Suddala +5 more
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eLife, 2018 Multi-subunit ring-shaped ATPases are molecular motors that harness chemical free energy to perform vital mechanical tasks such as polypeptide translocation, DNA unwinding, and chromosome segregation.
Ninning Liu +3 more
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Two-subunit DNA escort mechanism and inactive subunit bypass in an ultra-fast ring ATPase
eLife, 2015 SpoIIIE is a homo-hexameric dsDNA translocase responsible for completing chromosome segregation in Bacillus subtilis. Here, we use a single-molecule approach to monitor SpoIIIE translocation when challenged with neutral-backbone DNA and non-hydrolyzable ...
Ninning Liu +2 more
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Local-to-global signal transduction at the core of a Mn2+ sensing riboswitch
Nature Communications, 2019 Riboswitches bind intracellular metabolites and control bacterial gene expression. Here, by using X-ray crystallography, molecular dynamics simulations, and single-molecule fluorescence resonance energy transfer, the authors show how a local Mn2+ ion ...
Krishna C. Suddala +8 more
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Distribution bias and biochemical characterization of TOP1MT single nucleotide variants
Scientific Reports, 2017 Mitochondrial topoisomerase I (TOP1MT) is a type IB topoisomerase encoded in the nucleus of vertebrate cells. In contrast to the other five human topoisomerases, TOP1MT possesses two high frequency single nucleotide variants (SNVs), rs11544484 (V256I ...
Hongliang Zhang +4 more
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