Results 71 to 80 of about 26,343 (300)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Developing a New Machine-Learning Algorithm for Estimating Chlorophyll-a Concentration in Optically Complex Waters: A Case Study for High Northern Latitude Waters by Using Sentinel 3 OLCI

Remote Sensing, 2019
The monitoring of Chlorophyll-a (Chl-a) concentration in high northern latitude waters has been receiving increased focus due to the rapid environmental changes in the sub-Arctic, Arctic.
Katalin Blix, Juan Li, Philippe Massicotte, Atsushi Matsuoka   +3 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Revealing the mid-infrared emission structure of IRAS 16594-4656 and IRAS 07027-7934

, 2005
TIMMI2 diffraction-limited mid-infrared images of a multipolar proto-planetary nebula IRAS 16594-4656 and a young [WC] elliptical planetary nebula IRAS 07027-7934 are presented.
A. Benitez Canete, A. M. Garcia, A. Manchado, Allain T., Asplund M., Colangeli L., D. A. Garcia‐Hernandez, Herwig F., Herwig F., Herwig F., Hrivnak B. J., J. A. Acosta‐Pulido, Justtanont K., Leuenhagen U., Loup C., Menzies J. W., P. Garcia‐Lario, Pauzat F., Stasynska G., Te Lintel Hekkert P., Van de Steene G. C., Van de Steene G. C., van der Veen W. E. C. J., Yamamura I., Zijlstra A. A., Zijlstra A. A., Zijlstra A. A.   +26 more
core   +2 more sources

2-Dust : a Dust Radiative Transfer Code for an Axisymmetric System [PDF]

, 2002
We have developed a general purpose dust radiative transfer code for an axisymmetric system, 2-Dust, motivated by the recent increasing availability of high-resolution images of circumstellar dust shells at various wavelengths.
Cornette W. M., Dorschner J., Dullemond C. P., Efstathiou A., Jager C., Jager C., Lefevre J., Margaret Meixner, Menshchikov A. B., Siebenmorgen R., Toshiya Ueta, Waters L. B. F. M.   +11 more
core   +2 more sources

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

The Infrared Continuum Sizes of Be Star Disks

, 2011
We present an analysis of the near-infrared continuum emission from the circumstellar gas disks of Be stars using a radiative transfer code for a parametrized version of the viscous decretion disk model. This isothermal gas model creates predicted images
Bonanos, Carciofi, Carciofi, Coté, D. R. Gies, Dougherty, Elias, Floquet, G. H. Schaefer, Gies, Ginestet, Hummel, Kastner, Lamers, Lee, Okazaki, Porter, Porter, Quirrenbach, Rinehart, Schaefer, Sigut, Skrutskie, Sterken, Tanabé, Tycner, Tycner, Waters, Waters, Waters, Waters, Woolf, Y. Touhami   +32 more
core   +1 more source

Intercompartmental communication in senescence

FEBS Open Bio, EarlyView.
Senescent cells experience structural changes in the plasma membrane, endoplasmic reticulum, mitochondria, lysosomes, nucleus, and cytoskeleton. These alterations disrupt crosstalk among cellular compartments, impairing vesicular trafficking, contact sites, and molecular flow.
Krystyna Mazan‐Mamczarz, Eleanor J. Wind, Jixiang Leng, Myriam Gorospe   +3 more
wiley   +1 more source

Origins, evolutions, and future directions of Landsat science products for advancing global inland water and coastal ocean observations [PDF]

Earth System Science Data
In April 2020, the U.S. Geological Survey (USGS) Earth Resources Observation and Science (EROS) Center introduced a Level 2 provisional Aquatic Reflectance (AR) product for the Landsat 8 Operational Land Imager (OLI), marking the initial phase in ...
B. Page, C. J. Crawford, S. Arab, G. Schmidt, C. Barnes, D. Wellington   +5 more
doaj   +1 more source