Results 101 to 110 of about 323,365 (265)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Stress Distribution in Different Dental Provisional Restoration Materials with Different Posterior Connector Dimensions: A 3D Finite Element Analysis

open access: yesApplied Sciences
The aim of this in vitro study is to evaluate the stress distribution of different provisional materials designed with different connector dimensions using finite element analysis. Two adjacent prepped maxillary molars were designed digitally.
Turki S. Alkhallagi   +2 more
doaj   +1 more source

SCHEME ANALYSIS TREE DIMENSIONS AND TOLERANCES PROCESSING [PDF]

open access: yesFiabilitate şi Durabilitate, 2011
This paper presents one of the steps that help us to determine the optimal tolerances depending on thetechnological capability of processing equipment. To determine the tolerances in this way is necessary to takethe study and to represent schematically ...
Constanta RADULESCU   +2 more
doaj  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

On Geometric Shaping of Intensity-Modulated Signals for MIMO Optical Wireless Communications

open access: yesIEEE Photonics Journal
This paper investigates geometric shaping for multiple-input multiple-output optical wireless communications with intensity modulation and direct detection. We characterize the optimal shaping region for arbitrary-dimensional geometrically-shaped spatial-
Jingjing Zhang   +3 more
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

PENENTUAN BENTUK DAN UKURAN PLOT CONTOH OPTIMAL PENGUKURAN KEANEKARAGAMAN SPESIES TUMBUHAN DI HUTAN PEGUNUNGAN BAWAH

open access: yesMedia Konservasi, 2016
Several recent books have focused on the importance of biological diversity but none contain precise methodologies to measure it. The purpose of this study is to determine the shape and size optimal sampling plot to measure the plant species diversity in
M. Adlan Ali, Agus Hikmat, Yanto Santosa
doaj  

Peculiarities of Betavoltaic Battery Based on Si [PDF]

open access: yesЖурнал нано- та електронної фізики, 2014
In the paper the description of the optimal geometric dimensions of the structure of beta silicon galvanic battery using radioisotope Ni63 is presented.
V.N. Murashev   +4 more
doaj  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

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