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The AP2/ERF-domain transcription factor ORCA3 is a master regulator of primary and secondary metabolism in Catharanthus roseus (periwinkle). Here we demonstrate that ORCA3 specifically binds to and activates gene expression via a previously characterized jasmonate- and elicitor-responsive element (JERE) in the promoter of the terpenoid indole alkaloid ...
Johan Memelink
exaly +4 more sources
ORCA3, a Jasmonate-Responsive Transcriptional Regulator of Plant Primary and Secondary Metabolism
Science, 2000Biosynthesis of many classes of secondary metabolites in plants is induced by the stress hormone jasmonate. The gene for ORCA3, a jasmonate-responsive APETALA2 (AP2)-domain transcription factor from Catharanthus roseus , was isolated by transferred DNA activation tagging. Orca3 overexpression
Johan Memelink
exaly +3 more sources
The Jasmonate-Responsive Element from the ORCA3 Promoter from Catharanthus roseus is Active in Arabidopsis and is Controlled by the Transcription Factor AtMYC2 [PDF]
Jasmonates are plant signaling molecules that play key roles in protection against certain pathogens and against insects by switching on the expression of genes encoding defense proteins including enzymes involved in the biosynthesis of toxic secondary metabolites.
Gregory Montiel, Johan Memelink
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Catharanthus roseus produces many pharmaceutically important terpenoid indole alkaloids (TIAs) such as vinblastine, vincristine, ajmalicine, and serpentine. Past metabolic engineering efforts have pointed to the tight regulation of the TIA pathway and to multiple rate-limiting reactions.
Christie A M Peebles
exaly +3 more sources

