Results 151 to 160 of about 3,880 (206)

<i>Bacillus subtilis</i> Strain TCX1 Isolated from <i>Ambrosia artemisiifolia</i>: Enhancing Cucumber Growth and Biocontrol Against Cucumber <i>Fusarium</i> Wilt. [PDF]

Plants (Basel)
Dong Y, Zhu M, Zhao Y, Yi E, Zhang J, Wang Z, Wang C, Yu C, Ma L.   +8 more
europepmc   +1 more source

Applications of nanomaterials with enzyme-like activity for the detection of phytochemicals and hazardous substances in plant samples. [PDF]

Chin Med
Xu L, Luo ML, Dai JJ, Zhu H, Li P, Wang D, Yang FQ.   +6 more
europepmc   +1 more source

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Organophosphorus hydrolase multilayer modified microcantilevers for organophosphorus detection

Biosensors and Bioelectronics, 2007
We report a biosensor based on organophosphorus hydrolase (OPH) multilayer modified microcantilever (MCL) for detection of organophosphorus compounds (OPs). The assay is based on substrate-dependent bending of the OPH functionalized MCLs. The cantilever bending amplitude at equilibrium was a function of the concentration of paraoxon with the dynamic ...
Chandana, Karnati, Hongwei, Du, Hai-Feng, Ji, Xiaohe, Xu, Yuri, Lvov, Ashok, Mulchandani, Priti, Mulchandani, Wilfred, Chen   +7 more
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Biodegradation of organophosphorus pesticides by surface-expressed organophosphorus hydrolase

Nature Biotechnology, 1997
Organophosphorus hydrolase (OPH) was displayed and anchored onto the surface of Escherichia coli using an Lpp-OmpA fusion system. Production of the fusion proteins in membrane fractions was verified by immunoblotting with OmpA antisera. Inclusion of the organophosphorus hydrolase signal sequence was necessary for achieving enzymatic activity on the ...
R D, Richins, I, Kaneva, A, Mulchandani, W, Chen   +3 more
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Detection of Organophosphorus Compounds by Covalently Immobilized Organophosphorus Hydrolase

Analytical Chemistry, 2006
As a consequence of organophosphorus (OP) toxins posing a threat to human life globally, organophosphorus hydrolase (OPH) has become the enzyme of choice to detoxify such compounds. Organophosphorus hydrolase was covalently immobilized onto a quartz substrate for utilization in paraoxon detection.
Jhony, Orbulescu, Celeste A, Constantine, Vipin K, Rastogi, Saumil S, Shah, Joseph J, Defrank, Roger M, Leblanc   +5 more
openaire   +2 more sources

Serine hydrolase targets of organophosphorus toxicants

Chemico-Biological Interactions, 2005
Acetylcholinesterase (AChE) is one of several hundred serine hydrolases in people potentially exposed to about 80 organophosphorus (OP) compounds important as insecticides or chemical warfare agents. The toxicology of OPs was interpreted until recently almost solely on the basis of AChE inhibition.
John E, Casida, Gary B, Quistad
openaire   +2 more sources

Improving storage stability of recombinant organophosphorus hydrolase

Protein Expression and Purification, 2015
Organophosphorus hydrolase (OPH) is a ∼38kDa enzyme encoded by opd gene of Flavobacterium sp. The enzyme can hydrolyze and inactivate variety of organophosphate (OP)-compounds, including chemical warfare nerve agents. Thus, OPH is a strong candidate for the development of therapeutic intervention against OP-poisoning in humans and other animals.
A R, Satvik Iyengar, Rajan K, Tripathy, Priyanka, Bajaj, Abhay H, Pande   +3 more
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Organophosphorus Hydrolase-Based Assay for Organophosphate Pesticides

Biotechnology Progress, 1999
We report a rapid and versatile organophosphorus hydrolase (OPH)-based method for measurement of organophosphates. This assay is based on a substrate-dependent change in pH at the local vicinity of the enzyme. The pH change is monitored using fluorescein isothiocyanate (FITC), which is covalently immobilized to the enzyme.
, Rogers, , Wang, , Mulchandani, , Mulchandani, , Chen   +4 more
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Degradation of Organophosphorus Neurotoxicity in SY5Y Neuroblastoma Cells by Organophosphorus Hydrolase (OPH)

Journal of Toxicology and Environmental Health, Part A, 2006
Numerous approaches have been studied to degrade organophosphorus (OP) compounds and ameliorate their toxicity. In the current study, the potential of genetically engineered organophosphorus hydrolase (OPH) enzymes to functionally biotransform OP neurotoxicants was examined by assessing effects of OPH-hydrolyzed OPs on acute and delayed indicators of ...
Taehyeon M, Cho, James R, Wild, Kirby C, Donnelly, Evelyn, Tiffany-Castiglioni   +3 more
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Phytodegradation of organophosphorus compounds by transgenic plants expressing a bacterial organophosphorus hydrolase

Biochemical and Biophysical Research Communications, 2008
Organophosphorus (OP) compounds are widely used as pesticides in agriculture but cause broad-area environmental pollution. In this work, we have expressed a bacterial organophosphorus hydrolase (OPH) gene in tobacco plants. An assay of enzyme activity showed that transgenic plants could secrete OPH into the growth medium.
Xiaoxue, Wang, Ningfeng, Wu, Jun, Guo, Xiaoyu, Chu, Jian, Tian, Bin, Yao, Yunliu, Fan   +6 more
openaire   +2 more sources