Results 161 to 170 of about 3,880 (206)

Advances in detection of hazardous organophosphorus compounds using organophosphorus hydrolase based biosensors

Critical Reviews in Toxicology, 2019
Agricultural advancements focusing on increasing crop production have led to excessive usage of insecticides and pesticides, resulting in leaching and accumulation of these highly toxic chemicals in soil, water, and the food-chain. Organophosphorus (OP) compounds are the most commonly used insecticides and pesticides, which cause a wide range of long ...
Monika, Jain, Priyanka, Yadav, Abhijeet, Joshi, Prashant, Kodgire   +3 more
openaire   +2 more sources

Increasing the tolerance of organophosphorus hydrolase to bleach

Biotechnology & Bioengineering, 1999
Organophosphorus hydrolase (OPH) has been incorporated within polyurethane foams during polymer synthesis as a means of reducing the enzyme's environmental sensitivity to alterations in pH and bleach-induced enzyme denaturation. Unfavorable losses of enzyme activity upon altered pH are reduced by covalently incorporating OPH within polyurethane ...
, LeJeune, , Swers, , Hetro, , Donahey, , Russell   +4 more
openaire   +2 more sources

Enzymatic Hydrolysis of Russian-VX by Organophosphorus Hydrolase

Biochemical and Biophysical Research Communications, 1997
The Russian-VX (R-VX) is the principle V-type nerve agent in the chemical warfare (CW) arsenal of the Former Soviet Union. We here report the enzymatic hydrolysis of the P-S bond of Russian-VX by organophosphorus hydrolase (OPH) from Pseudomonas diminuta. While the Michaelis constant, K(m) for R-VX (474 microM), was similar to that for VX (434 microM),
V K, Rastogi, J J, DeFrank, T C, Cheng, J R, Wild   +3 more
openaire   +2 more sources

Surface Display of Organophosphorus Hydrolase on Saccharomyces cerevisiae

Biotechnology Progress, 2006
The gene encoding organophosphorus hydrolase (OPH) from Flavobacterium species was expressed on the cell surface of Saccharomyces cerevisiae MT8-1 using a glycosylphosphatidylinositol (GPI) anchor linked to the C-terminal region of OPH. Immunofluorescence microscopy confirmed the localization of OPH on the cell surface, and fluorescence intensity ...
Katsumi, Takayama, Shin-ichiro, Suye, Kouichi, Kuroda, Mitsuyoshi, Ueda, Tetsuya, Kitaguchi, Kouta, Tsuchiyama, Takeshi, Fukuda, Wilfred, Chen, Ashok, Mulchandani   +8 more
openaire   +2 more sources

Photoimmobilization of organophosphorus hydrolase within a PEG-based hydrogel

Biotechnology and Bioengineering, 1999
Organophosphorous hydrolase (OPH) was physically and covalently immobilized within photosensitive polyethylene glycol (PEG)-based hydrogels. The hydroxyl ends of branched polyethylene glycol (b-PEG, four arms, MW = 20,000) were modified with cinnamylidene acetate groups to give water-soluble, photosensitive PEG macromers (b-PEG-CA).
F M, Andreopoulos, M J, Roberts, M D, Bentley, J M, Harris, E J, Beckman, A J, Russell   +5 more
openaire   +2 more sources

Polyhistidine-containing organophosphorus hydrolase with outstanding properties

Biocatalysis and Biotransformation, 2007
The catalytic and physical–chemical properties of organophosphorus hydrolase (OPH) modified by the addition of an N-terminal dodecahistidine tag (His12-OPH) have been investigated. Introduction of the His12-tag caused a 30- and 74-fold increase in catalytic efficiency of the enzyme with parathion and methyl parathion, respectively, compared to OPH ...
Elena Efremenko, Ilya Lyagin, Yulia Votchitseva, Maria Sirotkina, Sergey Varfolomeyev   +4 more
openaire   +1 more source

Optical solid-state detection of organophosphates using organophosphorus hydrolase

Biosensors and Bioelectronics, 2005
We have developed a sensor surface for optical detection of organophosphates based on reversible inhibition of organophosphorus hydrolase (OPH) by copper complexed meso-tri(4-sulfonato phenyl) mono(4-carboxy phenyl) porphyrin (CuC1TPP). OPH immobilized onto glass microscope slides retains catalytic activity for more than 232 days.
Brandy J, White, H James, Harmon
openaire   +2 more sources

Organophosphate hydrolases as catalytic bioscavengers of organophosphorus nerve agents

Toxicology Letters, 2011
Bioscavengers are molecules able to neutralize neurotoxic organophosphorus compounds (OP) before they can reach their biological target. Human butyrylcholinesterase (hBChE) is a natural bioscavenger each molecule of enzyme neutralizing one molecule of OP. The amount of natural enzyme is insufficient to achieve good protection.
Marie, Trovaslet-Leroy, Lucie, Musilova, Frédérique, Renault, Xavier, Brazzolotto, Jan, Misik, Ladislav, Novotny, Marie-Thérèse, Froment, Emilie, Gillon, Mélanie, Loiodice, Laurent, Verdier, Patrick, Masson, Daniel, Rochu, Daniel, Jun, Florian, Nachon   +13 more
openaire   +2 more sources

Fatty Acid Amide Hydrolase Inhibition by Neurotoxic Organophosphorus Pesticides

Toxicology and Applied Pharmacology, 2001
Organophosphorus (OP) compound-induced inhibition of acetylcholinesterase (AChE) and neuropathy target esterase explains the rapid onset and delayed neurotoxic effects, respectively, for OP insecticides and related compounds but apparently not a third or intermediate syndrome with delayed onset and reduced limb mobility.
G B, Quistad, S E, Sparks, J E, Casida
openaire   +2 more sources

Rational design of organophosphorus hydrolase for altered substrate specificities

Chemico-Biological Interactions, 1999
Organophosphorus hydrolase (OPH) is a bacterial enzyme that hydrolyzes a broad variety of OP neurotoxins, including chemical warfare agents and many widely used pesticides. OPH has extremely high hydrolytic efficiency with different phosphotriester and phophothiolester pesticides (k(cat) = 50-15,000 s(-1)) as well as phosphorofluorates such as DFP and ...
B D, Di Sioudi, C E, Miller, K, Lai, J K, Grimsley, J R, Wild   +4 more
openaire   +2 more sources