Results 171 to 180 of about 3,880 (206)

Organophosphorus Hydrolase (OpdB) of Lactobacillus brevis WCP902 from Kimchi Is Able To Degrade Organophosphorus Pesticides

Journal of Agricultural and Food Chemistry, 2010
Lactobacillus brevis WCP902 that is capable of biodegrading chlorpyrifos was isolated from kimchi. The opdB gene cloned from this strain revealed 825 bp, encoding 274 aa, and an enzyme molecular weight of about 27 kDa. OpdB contains the same Gly-X-Ser-X-Gly motif found in most bacterial and eukaryotic esterase, lipase, and serine hydrolases, yet it is ...
Shah Md Asraful, Islam, Renukaradhya K, Math, Kye Man, Cho, Woo Jin, Lim, Su Young, Hong, Jong Min, Kim, Myoung Geun, Yun, Ji Joong, Cho, Han Dae, Yun   +8 more
openaire   +2 more sources

Decontamination of nerve agents by immobilized organophosphorus hydrolase

Forensic Toxicology, 2012
Organophosphorus hydrolase (OPH; EC 3.1.8.1) is known to be capable of hydrolyzing a variety of organophosphorus compounds, such as sarin and paraoxon. We have developed a nerve agent decontamination method using OPH. The gene that encodes OPH was cloned from the bacterial strain Sphingobium fuliginis ATCC 27551, and several OPH gene fusion plasmids ...
Takeshi Ohmori, Kazuyoshi Kawahara, Kosuke Nakayama, Aki Shioda, Satoshi Ishikawa, Mieko Kanamori-Kataoka, Shintaro Kishi, Asuka Komano, Yasuo Seto   +8 more
openaire   +1 more source

Novel fungal organophosphorus hydrolases in acidic media: an application to apples decontamination

Environmental Science and Pollution Research, 2022
Organophosphorus pesticides bring significant improvements in agriculture, but their toxicity causes environmental and health negative impacts. The aim of this work was the development of robust biocatalysts to be applied in bioremediation. Four fungi were evaluated as hydrolase sources capable of degrading organophosphorus pesticides: Aspergillus ...
Julia Yamila Santillan, Natalia Lorena Rojas, Elizabeth Sandra Lewkowicz, Adolfo Marcelo Iribarren   +3 more
openaire   +3 more sources

Active Site Modifications of Organophosphorus Hydrolase for Improved Detoxification of Organophosphorus Neurotoxins

2000
Amino acid substitutions within the active site of the dimeric metalloenzyme Organophosphorus Hydrolase (OPH) result in a striking enhancement in the hydrolysis of certain chemical warfare agents and their analogues. These changes alter the metal content of the enzyme and we suggest that changes in metal requirements improve the catalytic ...
Janet K. Grimsley, Barbara D. Disioudi, Thomas R. Holton, James C. Sacchettini, James R. Wild   +4 more
openaire   +1 more source

Expression of organophosphorus hydrolase OPHC2 in Pichia pastoris: Purification and characterization

Protein Expression and Purification, 2006
Organophosphorus hydrolase is able to hydrolyze phosphoester bonds and reduce the toxicity of organophosphorus compounds. In this work, recombinant organophosphorus hydrolase OPHC2 was successfully produced by Pichia pastoris at a high expression level (approximately 5.5 g/L) using 3 L high-cell-density fermentation. The expression level is higher than
Xiao-Yu, Chu, Ning-Feng, Wu, Min-Jie, Deng, Jian, Tian, Bin, Yao, Yun-Liu, Fan   +5 more
openaire   +4 more sources

Diversity of organophosphorus pesticide-degrading bacteria in a polluted soil and conservation of their organophosphorus hydrolase genes

Canadian Journal of Microbiology, 2005
Seven methyl parathion-degrading bacteria were isolated from a long-term methyl parathion contaminated soil and were found to belong to the genera Pseudaminobacter, Achromobacter, Brucella, and Ochrobactrum. Southern blot analysis using an mpd gene probe revealed that their hydrolase genes were similar to the mpd gene from Plesiomonas sp. strain M6 and
Ruifu, Zhang, Zhongli, Cui, Jiandong, Jiang, Jian, He, Xiangyang, Gu, Shunpeng, Li   +5 more
openaire   +2 more sources

Enzymatic bioremediation of organophosphorus insecticides by recombinant organophosphorous hydrolase

International Biodeterioration & Biodegradation, 2011
Abstract The gene for organophosphorous hydrolase (OPH) (GenBank accession no. M20392 ) was chemically synthesised with a codon bias toward E. coli , followed by its cloning and heterologous over-expression in E. coli under induction with 0.1 M isopropyl- d -thiogalactopyranoside (IPTG).
Mukesh Kapoor, R. Rajagopal
openaire   +1 more source

Organophosphorus hydrolase as an in vivo catalytic nerve agent bioscavenger

Drug Testing and Analysis, 2012
The use of proteins as a treatment for organophosphorus intoxication has been investigated since A. R. Main demonstrated protective efficacy against parathion with an exogenously administered arylesterase in the late 1950s. His experiments spurred over 60 years of research and progress in the development of enzymes as potential bioscavengers of nerve ...
Melinda E, Wales, Tony E, Reeves
openaire   +2 more sources

Bioassay Development using Organophosphorus Hydrolase for Detection of Paraoxon

ECS Meeting Abstracts, 2006
Abstract not Available.
Roger Leblanc, Celeste Constantine, Xiaojun Ji, Jhony Orbulescu   +3 more
openaire   +1 more source

Improved Degradation of Organophosphorus Nerve Agents and p-Nitrophenol by Pseudomonas putida JS444 with Surface-Expressed Organophosphorus Hydrolase

Biotechnology Progress, 2008
Pseudomonas putida JS444, isolated from p-nitrophenol (PNP) contaminated waste sites, was genetically engineered to simultaneously degrade organophosphorus pesticides (OP) and PNP. A surface anchor system derived from the ice-nucleation protein (INP) from Pseudomonas syringae was used to target the organophosphorus hydrolase (OPH) onto the surface of ...
Yu, Lei, Ashok, Mulchandani, Wilfred, Chen   +2 more
openaire   +2 more sources