Results 11 to 20 of about 3,880 (206)

Enhancing Paraoxon Binding to Organophosphorus Hydrolase Active Site. [PDF]

Int J Mol Sci, 2021
Organophosphorus hydrolase (OPH) is a metalloenzyme that can hydrolyze organophosphorus agents resulting in products that are generally of reduced toxicity. The best OPH substrate found to date is diethyl p-nitrophenyl phosphate (paraoxon). Most structural and kinetic studies assume that the binding orientation of paraoxon is identical to that of ...
El Khoury L, Mobley DL, Ye D, Rempe SB.
europepmc   +7 more sources

Efficacy of an organophosphorus hydrolase enzyme (OpdA) in human serum and minipig models of organophosphorus insecticide poisoning. [PDF]

Clin Toxicol (Phila), 2020
Objectives: Current therapeutic options for organophosphorus (OP) insecticide self-poisoning including atropine and oximes are inadequate and case fatality may exceed 20%. An OP hydrolase enzyme, OpdA, has been used for environmental cleansing of OP insecticides and prevented death in rat and non-human primate models of OP insecticide poisoning if ...
Eddleston M, Clutton RE, Taylor M, Thompson A, Worek F, John H, Thiermann H, Scott C.   +7 more
europepmc   +5 more sources

Acetylcholinesterase: Structure, dynamics, and interactions with organophosphorus compounds. [PDF]

Protein Sci
Abstract Acetylcholinesterase (AChE) is an enzyme that hydrolyzes the neurotransmitter acetylcholine (ACh), removing it from the synaptic cleft after the transmission of an electrical signal, making it an essential component of chemical neurotransmission. AChE is a serine hydrolase, containing a catalytic triad of Ser/His/Glu.
Hung LW, Sanbonmatsu KY, Williams RF, Chen JC.   +3 more
europepmc   +2 more sources

Multiple acetylcholinesterases in Pardosa pseudoannulata brain worked collaboratively to provide protection from organophosphorus insecticides

Ecotoxicology and Environmental Safety, 2022
Acetylcholinesterase (AChE) is an essential neurotransmitter hydrolase in nervous systems of animals and its number varies among species. So far, five AChEs have been identified in the natural enemy Pardosa pseudoannulata.
Xumin Lin, Yixi Zhang, Baojun Yang, Lingchun Zhang, Yunru Chen, Zewen Liu   +5 more
doaj   +1 more source

Pharmacokinetics of OpdA, an organophosphorus hydrolase, in the African green monkey. [PDF]

Biochem Pharmacol, 2010
Organophosphorus (OP) pesticides are a broad class of acetylcholinesterase inhibitors that are responsible for tremendous morbidity and mortality worldwide, contributing to an estimated 300,000 deaths annually. Current pharmacotherapy for acute OP poisoning includes the use of atropine, an oxime, and benzodiazepines.
Jackson CJ, Scott C, Carville A, Mansfield K, Ollis DL, Bird SB.   +5 more
europepmc   +5 more sources

3D Bioprinted Engineered Living Microreactors for Continuous Organophosphorus Compound Degradation. [PDF]

Small Sci
3D bioprinted engineered living microreactors offer a novel solution for continuous organophosphorus compound (OPC) degradation. This work details the fabrication and characterization of porous microbial ELM bioreactors containing genetically engineered Escherichia coli for cyclic OPC detoxification.
Shannon MR, Day GJ, Bloomfield-Gadêlha H, Ting VP, Perriman AW.   +4 more
europepmc   +2 more sources

Yeast-Based Biotechnology for Civilian Security. [PDF]

Environ Microbiol Rep
Yeasts constitute robust and versatile eukaryotic platforms for biotechnological applications relevant to civilian security. Both native and genetically engineered yeast systems enable sensitive detection of chemical and biological hazards, effective removal of metals and organic pollutants, and detoxification of mycotoxins.
Ruchała J, Vasylyshyn R, Wnuk M.
europepmc   +2 more sources

Export of functional Streptomyces coelicolor alditol oxidase to the periplasm or cell surface of Escherichia coli and its application in whole-cell biocatalysis [PDF]

, 2009
Streptomyces coelicolor A3(2) alditol oxidase (AldO) is a soluble monomeric flavoprotein in which the flavin cofactor is covalently linked to the polypeptide chain.
AJ Driessen, BJ Feilmeier, C Filip, CA Kumamoto, CF Schierle, CM Cho, CR Raetz, D Huber, D Schmid, D Tullman-Ercek, DP Heuts, E Brickman, E Frirdich, E Vrontou, EW van Hellemond, F Forneris, FJ Mergulhao, HC Jung, J Jose, J Luirink, J Zhou, JD Thomas, JS Lee, K Nakai, L Li, M Marrichi, M Shimazu, M Shimazu, MA Turner, MJ Casadaban, MJ Casadaban, ML Wu, MW Fraaije, NG Leferink, P Samuelson, PA Lee, PH Wu, R Federico, S Cristobal, SK Yim, SM Kang, SP Graether, SY Lee, T Baba, T Bruser   +44 more
core   +9 more sources

Biodegradation of Organophosphorus Pesticide (Malathion) by Bacillus sp. FYM31 Isolated from Agriculture Drainage Water

Journal of Scientific Research in Science, 2022
Organophosphorus pesticides (OP) are used extensively in many arenas including agriculture and industry leading to humans and agroecosystems disorders. Malathion is one of the OP that are used in agriculture to control pest and protect crops.
Walaa N. Madbolly, Manal F. Abdelall, Sanaa S. Zaki, Hanan A. Nour El-Din, Mona I. Fahd, Soad A. Abdallah   +5 more
doaj   +1 more source

Improving Catalytic Activity and Thermal Stability of Methyl-Parathion Hydrolase for Degrading the Pesticide of Methyl-Parathion

International Journal of Chemical Engineering, 2022
Pesticides are indispensable in today’s agriculture. Methyl-parathion hydrolase (MPH, E.C.3.1.8.1) could hydrolyze organophosphorus pesticides, including methyl-parathion.
Cheng Shi, Song Liu, Guocheng Du
doaj   +1 more source