Results 91 to 100 of about 5,350,711 (346)

Origin of life from a maker's perspective -- focus on protocellular compartments in bottom-up synthetic biology [PDF]

open access: yesarXiv, 2022
The origin of life is shrouded in mystery, with few surviving clues, obscured by evolutionary competition. Previous reviews have touched on the complementary approaches of top-down and bottom-up synthetic biology to augment our understanding of living systems.
arxiv  

Cytosolic‐enhanced dark Epac‐based FRET sensors allow for intracellular cAMP detection in live cells via FLIM

open access: yesFEBS Letters, EarlyView.
We describe a novel set of Epac‐based FRET‐FLIM biosensors with improved fully cytosolic distribution, achieved without compromising the state‐of‐the‐art performance of our original designs, for detecting cAMP dynamics in real time in live cells with high precision and reliability.
Giulia Zanetti   +2 more
wiley   +1 more source

Exobiology and the origin of life [PDF]

open access: yes
s on planetary studies and the search for extraterrestrial life are presented. Studies of the Jovian atmosphere were conducted. An assessment of the prospects for life on Mars is presented.
Khare, B. N., Sagan, C.
core   +4 more sources

From Protoplanets to Protolife: The Emergence and Maintenance of Life [PDF]

open access: yesarXiv, 2006
Despite great advances in our understanding of the formation of the Solar System, the evolution of the Earth, and the chemical basis for life, we are not much closer than the ancient Greeks to an answer of whether life has arisen and persisted on any other planet.
arxiv  

Diphthamide synthesis is linked to the eEF2‐client chaperone machinery

open access: yesFEBS Letters, EarlyView.
The diphthamide modification of eukaryotic translation elongation factor 2 (eEF2) is important for accurate protein synthesis. We addressed the potential coordination of de novo eEF2 synthesis with simultaneous or subsequent diphthamide modification. Our work reveals that the co‐chaperones Hgh1 and Cpr7, which are known to support folding of nascent ...
Lars Kaduhr   +4 more
wiley   +1 more source

The [2Fe‐2S] cluster of mitochondrial outer membrane protein mitoNEET has an O2‐regulated nitric oxide access tunnel

open access: yesFEBS Letters, EarlyView.
The mitochondrial outer membrane iron–sulphur ([Fe‐S]) protein mitoNEET is a target of the type‐2 diabetes drug pioglitazone. Its unknown molecular function is linked to respiratory complex activity and mitochondrial function. We discovered that O2 protects the mitoNEET [2Fe‐2S] cluster against NO oxidation and desensitization towards reduction by H2S.
Thao Nghi Hoang   +9 more
wiley   +1 more source

Recent Progress in Chirality Research Using Circularly Polarized Light [PDF]

open access: yesPhotochemistry: UV/VIS Spectroscopy, Photochemical Reactions and Photosynthesis: Nova Science Publishers (2011), pp.291-308, 2010
(abridged) We review recent studies of chirality using circularly polarized light, along with the birth and evolution of life and planetary systems. Terrestrial life consists almost exclusively of one enantiomer, left-handed amino acids. This characteristic feature is called homochirality, whose origin is still unknown.
arxiv  

Characterization of fungal carbonyl sulfide hydrolase belonging to clade D β‐carbonic anhydrase

open access: yesFEBS Letters, EarlyView.
Here, we performed a functional analysis of the fungal enzymes belonging to clade D of the β‐class carbonic anhydrase family (β‐D‐CA). The β‐D‐CAs in the basidiomycete Gloeophyllum trabeum and the ascomycete Trichoderma harzianum showed very low activity in the hydration of CO2 but exhibited high activity in the hydrolysis of carbonyl sulfide (COS ...
Ryuka Iizuka   +6 more
wiley   +1 more source

Quality and nutritional properties of corn snacks enriched with nanofiltered whey powder

open access: yesCzech Journal of Food Sciences, 2016
Corn snacks containing 0, 3, 5, and 10% of nanofiltered whey powder, obtained from the raw material of 12 and 14% moisture contents were analysed. Colour, texture, sensory properties, protein, available lysine, and acrylamide contents were determined in ...
Agnieszka Makowska   +4 more
doaj   +1 more source

The origins of cellular life [PDF]

open access: yesAntonie van Leeuwenhoek, 2014
All life on earth can be naturally classified into cellular life forms and virus-like selfish elements, the latter being fully dependent on the former for their reproduction. Cells are reproducers that not only replicate their genome but also reproduce the cellular organization that depends on semipermeable, energy-transforming membranes and cannot be ...
openaire   +3 more sources

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