Results 21 to 30 of about 84,971 (337)

A versatile enhanced freeze-substitution protocol for volume electron microscopy

Frontiers in Cell and Developmental Biology, 2022
Volume electron microscopy, a powerful approach to generate large three-dimensional cell and tissue volumes at electron microscopy resolutions, is rapidly becoming a routine tool for understanding fundamental and applied biological questions.
Sébastien Bélanger, Heather Berensmann, Heather Berensmann, Valentina Baena, Valentina Baena, Keith Duncan, Blake C. Meyers, Blake C. Meyers, Kedar Narayan, Kedar Narayan, Kirk J. Czymmek, Kirk J. Czymmek   +11 more
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In situ X-ray-assisted electron microscopy staining for large biological samples

eLife, 2022
Electron microscopy of biological tissue has recently seen an unprecedented increase in imaging throughput moving the ultrastructural analysis of large tissue blocks such as whole brains into the realm of the feasible.
Sebastian Ströh, Eric W Hammerschmith, David W Tank, H Sebastian Seung, Adrian Andreas Wanner   +4 more
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Reactional Processes on Osmium–Polymeric Membranes for 5–Nitrobenzimidazole Reduction

Membranes, 2021
Membranes are associated with the efficient processes of separation, concentration and purification, but a very important aspect of them is the realization of a reaction process simultaneously with the separation process.
Aurelia Cristina Nechifor, Alexandru Goran, Vlad-Alexandru Grosu, Andreia Pîrțac, Paul Constantin Albu, Ovidiu Oprea, Alexandra Raluca Grosu, Dumitru Pașcu, Florentina Mihaela Păncescu, Gheorghe Nechifor, Szidonia-Katalin Tanczos, Simona Gabriela Bungău   +11 more
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2D and 3D immunogold localization on (epoxy) ultrathin sections with and without osmium tetroxide

Microscopy research and technique (Print), 2020
For nearly 50 years immunogold labeling on ultrathin sections has been successfully used for protein localization in laboratories worldwide. In theory and in practice, this method has undergone continual improvement over time. In this study, we carefully
J. Flechsler, T. Heimerl, Carolin Pickl, R. Rachel, Y. Stierhof, A. Klingl   +5 more
semanticscholar   +1 more source

Distribution and behavior of lipid droplets in hepatic cells analyzed by variations of citochemical technique and scanning electron microscopy

MethodsX, 2022
Toxicity evaluations involve the analysis of multiple biomarkers. In this study, the liver, target organ analyzed by treatments with iron concentrations, indicated the accumulation of lipids as a response.
João Marcos de Lima-Faria, Lucas Nunes Guimarães, Victória Costa da Silva, Iara da Costa Souza, Marisa Narciso Fernandes, Diego Stéfani Teodoro Martinez, Simone Maria Teixeira de Sabóia-Morais   +6 more
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Regularities of plaque stabilization in various scenarios of neointimal calcification and vascularization

Российский кардиологический журнал, 2021
Aim. To study the relationships between phenotypes of extracranial arteries' plaques (stable/unstable), their calcification and its causes, in particular, vascularization.Material and methods. The study included 88 patients: patients (n=44) with ischemic
N. Yu. Osyaev, L. A. Bogdanov, R. A. Mukhamadiyarov, A. R. Shabaev, D. K. Shishkova, V. E. Markova, O. V. Gruzdeva, A. G. Kutikhin   +7 more
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Improved techniques for studies of adipocyte cellularity and metabolism1

Journal of Lipid Research, 1977
Two methods are described for the study of adipose tissue cellularity and metabolism. In the first, 8 M urea was used to liberate osmium tetroxide-fixed adipocytes from the connective tissue matrix.
T D Etherton, E H Thompson, C E Allen
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Obtaining and Characterizing the Osmium Nanoparticles/n–Decanol Bulk Membrane Used for the p–Nitrophenol Reduction and Separation System

Membranes, 2022
Liquid membranes based on nanoparticles follow a continuous development, both from obtaining methods and characterization of techniques points of view. Lately, osmium nanoparticles have been deposited either on flat membranes, with the aim of initiating ...
Aurelia Cristina Nechifor, Alexandru Goran, Szidonia-Katalin Tanczos, Florentina Mihaela Păncescu, Ovidiu-Cristian Oprea, Alexandra Raluca Grosu, Cristian Matei, Vlad-Alexandru Grosu, Bogdan Ștefan Vasile, Paul Constantin Albu   +9 more
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Assessment of the Effect of Secondary Fixation on the Structure of Meat Products Prepared for Scanning Electron Microscopy

Foods, 2020
The aim of the research was to verify the necessity of secondary fixation with osmium tetroxide in various types of meat products and evaluation of structural changes of products using different fixation procedures.
Hana Běhalová, Bohuslava Tremlová, Ludmila Kalčáková, Matej Pospiech, Dani Dordevic   +4 more
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Osmium Nanoparticles-Polypropylene Hollow Fiber Membranes Applied in Redox Processes

Nanomaterials, 2021
Composite membranes play a very important role in the separation, concentration, and purification processes, but especially in membrane reactors and membrane bioreactors.
Gheorghe Nechifor, Florentina Mihaela Păncescu, Alexandra Raluca Grosu, Paul Constantin Albu, Ovidiu Oprea, Szidonia-Katalin Tanczos, Constantin Bungău, Vlad-Alexandru Grosu, Andreia Pîrțac, Aurelia Cristina Nechifor   +9 more
doaj   +1 more source