Results 261 to 270 of about 99,961 (306)

Penicillin acylase extraction by osmotic shock

Process Biochemistry, 1992
Penicillin acylase was extracted from E. Coli by osmotic shock. The process was optimized by factorial design, scaled up and integrated to a purification process in order to compare it with purification processes reported in the literature. A specific parameter PPEF (Purification Processes Evaluation Factor) was defined for this purpose.
M. Rodriguez, L. Güereca, F. Valle, R. Quintero, A. López-Munguia   +4 more
openaire   +1 more source

Molecular Response to Osmotic Shock

2007
The cellular responses of cultured mammalian cells and non-mammalian organisms to changes in osmolarity are discussed. A number of common themes including activation of protein kinase cascades can be observed in a diverse group of organisms. A combination of physiological and transcriptional studies has been performed to identify regulatory factors and
Susan T. Sharfstein, Duan Shen, Thomas R. Kiehl, Rui Zhou   +3 more
openaire   +1 more source

Effect of osmotic shock on osmotolerant yeasts

Food / Nahrung, 1991
AbstractShock exposure of osmotolerant yeasts Debaryomyces hansenii 3339, Zygosaccharomyces mellis 3296 and Kloeckera antilarrum 3046 to solutions of sucrose at water activity (Aw) values between 0.99 and 0.88 was found to cause a great decrease in cell volume but an increase in heat resistance.
Deepa Bhajekar, P. R. Kulkarni
openaire   +1 more source

ACh release from osmotically shocked synaptosomes refilled with transmitter

Nature, 1981
It is still uncertain whether or not acetylcholine (ACh) released at the synapse is derived from vesicles. We have recently described a sensitive chemiluminescent technique which allows continuous measurement of ACh release from tissue slices or synaptosomes incubated in the reaction mixture1,2.
M, Israël, B, Lesbats, R, Manaranche
openaire   +2 more sources

Growth of Protein Crystals in Hydrogels Prevents Osmotic Shock

Journal of the American Chemical Society, 2012
High-throughput protein X-ray crystallography offers a significant opportunity to facilitate drug discovery. The most reliable approach is to determine the three-dimensional structure of the protein-ligand complex by soaking the ligand in apo crystals.
Sugiyama, S., Maruyama, M., Sazaki, G., Hirose, M., Adachi, H., Takano, K., satoshi murakami, Inoue, T., Mori, Y., Matsumura, H.   +9 more
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Ultrastructural features of osmotic shock in mussel gill cilia

Journal of Ultrastructure Research, 1977
Terminal gill cilia of the freshwater mussel Unio stop abruptly within 5 min (shock-stop) when distal gill filaments are placed in hypertonic (>217 ± mOsm) salt, urea or sucrose solutions. Electron microscopy of stopped cilia reveals that the axonemal complexes are obscured by an electron-dense matrix, although microtubule protofilaments are visible ...
K H, Kilburn, R A, Hess, R J, Thurston, T J, Smith   +3 more
openaire   +2 more sources

Characterization of Escherichia coli nucleoids released by osmotic shock

Journal of Structural Biology, 2012
Nucleoids were isolated by osmotic shock from Escherichia coli spheroplasts at relatively low salt concentrations and in the absence of detergents. Sucrose-protected cells, made osmotically sensitive by growth in the presence of ampicillin or by digestion with low lysozyme concentrations (50-5 μg/ml), were shocked by 100-fold dilution of the sucrose ...
Wegner, A.S., Alexeeva, S., Odijk, T., Woldringh, C.L.   +3 more
openaire   +4 more sources

Steroid-receptor in pseudomonas testosteroni released by osmotic shock

Journal of Steroid Biochemistry, 1973
Abstract An inducible steroid-binding activity, demonstrated in cultures of Pseudomonas testosteroni , was released into the medium after exposure of bacteria to osmotic shock. The protein nature of the binding activity released into the osmotic shock fluid was indicated by its susceptibility to pronase digestion and heat denaturation and resistance
M, Watanabe, K, Phillips, T, Chen
openaire   +2 more sources

Effect of osmotic shocks on rabbit kidney cortex slices

American Journal of Physiology-Renal Physiology, 1983
Rabbit kidney cortex slices behave an osmometers when withstanding hyperosmotic or hyposmotic shocks of amplitude up to pi 1/pi 2 = 1.25. For hyposmotic shocks of amplitude larger than or equal to pi 1/pi 2 = 1.50, the maximum swelling achieved is less than what can be expected on the basis of the van't Hoff relation, thereby indicating that a volume ...
R, Gilles, C, Duchene, I, Lambert
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Physical Responses of Yeast Cells to Osmotic Shock

Journal of Applied Bacteriology, 1975
Shock exposure of the osmophilic yeasts Saccharomyces rouxii, Torulopsis globosa and T. apicola to solutions of sucrose and polyethylene glycol of MW 200 (PEG 200) at water activity ( a w
openaire   +2 more sources