Results 131 to 140 of about 343,843 (272)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Contribution of Social Science Electives to Engineering Students

, 2014
Even though at first sight engineers are concerned solely with technology, they are superiors of a number of employees, whom they should handle wisely.

core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Applications of cross-sectional methodologies in developmental psychology

, 2015
Book synopsis: Fully revised and updated, the second edition of the International Encyclopedia of the Social and Behavioral Sciences, first published in 2001, offers a source of social and behavioral sciences reference material that is broader and deeper
Thomas, Michael S.C., Purser, Harry, Knowland, Victoria C.P.   +2 more
core  

Social categorization

, 2015
Categorization is a process by which people make sense of things by working out similarities and differences. Social categorization is especially important because it explains the way that people make sense of other people and themselves. Studying social
McGarty, Craig, Kenneth I. Mavor, Craig McGarty, Skorich, Daniel P, McGarty, Craig (R17770), Daniel P. Skorich, Skorich, Daniel P., Mavor, Kenneth I.   +7 more
core   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

DISCOVER: a Data-driven Interactive System for Comprehensive Observation, Visualization, and ExploRation of human behavior

Frontiers in Digital Health
Understanding human behavior is a fundamental goal of social sciences, yet conventional methodologies are often limited by labor-intensive data collection and complex analyses.
Tobias Hallmen, Dominik Schiller, Antonia Vehlen, Steffen Eberhardt, Tobias Baur, Daksitha Withanage Don, Wolfgang Lutz, Elisabeth André   +7 more
doaj   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

National Institute of Mental Health Roundtable Discussion: Promissory Notes and Prevailing Norms in Social and Behavioral Sciences Research

, 1997
Most workshops convened by the National Institute's of Health are devoted to the puzzle-solving activities of normal science, where the puzzles themselves and the strategies available for solving them are determined largely in advance by the shared ...
Richters, John E.
core