Results 191 to 200 of about 995,312 (298)

Challenges in meeting the mass transfer demands of high‐density perfusion cultures in single‐use bioreactors

open access: yesBiotechnology Progress, EarlyView.
Abstract Increasing protein therapeutics production by adopting intensified perfusion cultures places high demand on the mass transfer capabilities of single‐use bioreactors (SUB). Here, we compare these capabilities for four SUB designs against worst‐case O2 transfer requirements for supporting Chinese hamster ovary (CHO) perfusion cultures. Impact of
Anita B. Dabek   +9 more
wiley   +1 more source

Flocculation using selected cationic polymers reduces high‐risk host cell proteins in monoclonal antibody processes already during primary recovery

open access: yesBiotechnology Progress, EarlyView.
Flocculation using cationic polymers leads to the creation of large floc complexes. Negative charged species like cells, cell debris, and negatively charged host cell impurities like host cell DNA and host cell proteins are trapped within the complex while the positively charged monoclonal antibody stays in solution.
Anna‐Carina Frank   +9 more
wiley   +1 more source

The Asn680Ser Variant Negatively Impacted the Ovarian Response to Controlled Ovarian Stimulation in Thai Female Infertility. [PDF]

open access: yesIn Vivo
Boonlong N   +7 more
europepmc   +1 more source

Halogenated Tryptophans Improve Integrin αVβ6 Affinity of Cyclic RGD Peptides and Provide Handles for Late‐Stage Derivatization

open access: yesChemistry – A European Journal, EarlyView.
Lighting up αVβ6: Halotryptophan engineering converts an RGD scaffold into a modular αVβ6 ligand platform, enabling picomolar affinity and late‐stage functionalization. The resulting probes combine high selectivity with fluorescence for FACS and tumor imaging.
Beate Nachtigall   +4 more
wiley   +1 more source

LH supplementation in ovarian stimulation: propensity score and generalized estimating equations analysis over 2000 embryos. [PDF]

open access: yesFront Endocrinol (Lausanne)
Dizdar M   +8 more
europepmc   +1 more source

Opsoclonus in Pediatric Patients: Differential Diagnosis and a Practical Approach to Evaluation

open access: yesAnnals of the Child Neurology Society, EarlyView.
ABSTRACT Opsoclonus is an ocular dyskinesia characterized by involuntary, arrhythmic, multidirectional saccades. In pediatrics, opsoclonus is most commonly attributed to the rare neuroinflammatory disorder opsoclonus‐myoclonus‐ataxia syndrome (OMAS), typically considered a paraneoplastic syndrome associated with neural crest tumors. However, opsoclonus
Aubrey C. Reed   +5 more
wiley   +1 more source

Fhod3 in zebrafish supports myofibril stability during growth of embryonic skeletal muscle

open access: yesDevelopmental Dynamics, EarlyView.
Abstract Background Actin filament organization in cardiomyocytes critically depends on the formin Fhod3, but a role for Fhod3 in skeletal muscle development has not yet been described. Results We demonstrate here that in zebrafish mutated for one of two fhod3 paralog genes, fhod3a, skeletal muscle of the trunk appears normal through 2 days post ...
Aubrie Russell   +3 more
wiley   +1 more source

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