Results 271 to 280 of about 91,003 (373)

A short peptide derived from late embryogenesis abundant proteins enhances acid tolerance in Escherichia coli via modulation of two‐component regulatory systems

open access: yesThe FEBS Journal, EarlyView.
Expression of the LEA‐K peptide in E. coli BL21 (DE3) improved bacterial viability under acidic stress (pH4), suggesting a protective function. RNA‐sequencing of LEA‐K‐expressing vs. non‐expressing cells revealed 283 differentially expressed genes (DEGs). In silico docking linked LEA‐K to two‐component systems (TCS) proteins (Rcs, BtsS/R, and EnvZ/OmpR)
Khaled Metwally, Shinya Ikeno
wiley   +1 more source

Heterologous plastoquinone production using a newly identified O2‐dependent cyanobacterial hydroxylase

open access: yesThe FEBS Journal, EarlyView.
In this study, we confirm that PlqH is the hydroxylase operating in plastoquinone biosynthesis in photosynthetic cyanobacteria (Cyanobacteriia). Our phylogenetic analyses demonstrate that cyanobacterial PlqH homologues originated from hydroxylases involved in ubiquinone biosynthesis in bacteria. Plastoquinone production in Escherichia coli was achieved
Morgane Roger‐Margueritat   +7 more
wiley   +1 more source

The role of metabolism in shaping enzyme structures over 400 million years. [PDF]

open access: yesNature
Lemke O   +15 more
europepmc   +1 more source

Distribution and activity of nitrate and nitrite reductases in the microbiota of the human intestinal tract

open access: yesThe FEBS Journal, EarlyView.
Nitrate from food is first converted to nitrite by oral bacteria and then further processed in the intestine. The gut microbiota reduces nitrate and nitrite to ammonia or nitric oxide, preventing the formation of carcinogenic nitrosamines. Our analysis highlights Escherichia coli as a key player in this detoxification process, supported by other ...
Natalie Hager   +4 more
wiley   +1 more source

Zebrafish as a model for Catel–Manzke syndrome—identification and characterization of the zebrafish TGDS ortholog

open access: yesThe FEBS Journal, EarlyView.
Zebrafish Tgds, when expressed as a recombinant protein, catalyzes the dehydration of UDP‐D‐glucose, the initial step in the formation of 6‐deoxyhexoses. Corresponding Tgds mutations found in Catel–Manzke syndrome patients lead to reduced enzymatic activity and stability.
Maria Rosaria Coppola   +11 more
wiley   +1 more source

Plant organelle C‐to‐U RNA editing factors can operate successfully in yeast (Saccharomyces cerevisiae) as an easily amenable eukaryotic system for their functional analysis

open access: yesThe FEBS Journal, EarlyView.
Plant‐type pentatricopeptide repeat proteins capable of C‐to‐U RNA editing perform faithfully when expressed in a new heterologous system, the yeast Saccharomyces cerevisiae. They were tested with constitutive and inducible expression and with a set of different solubility tags. PPR56, PPR65, and PPR78 from P.
Shyam Ramanathan   +4 more
wiley   +1 more source

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