Results 121 to 130 of about 1,164 (177)
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Precipitation of Oxyhemoglobins A and S by Isopropanol
Hemoglobin, 1981The isopropanol precipitation test is widely used for the detection of unstable hemoglobins. A method for accurately and easily determining the amount of hemoglobin precipitated is described. This study demonstrates that changes in incubation time, pH, temperature, and isopropanol and hemoglobin concentrations affect the precipitation of oxy Hb A and ...
Kazuhiko Adachi, Toshio Asakura
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FORMATION OF METHEMOGLOBINS AND PEROXIDE FROM THE REACTION OF OXYHEMOGLOBINS WITH REDUCING AGENTS
, 1979 Winslow S Caughey
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Effect of organic phosphates on the sulfhydryl reactivities of oxyhemoglobins A and S. [PDF]
Journal of Biological Chemistry, 1980 The /393 sulfhydryl groups of oxyhemoglobins A and S display a difference in reactivity with 5,5’-dithiobis- 2-nitrobenzoic acid. It is concluded that this difference arises from differences in tertiary structure in the vicinity of the p”“ site ...
Okonjo, K. O.
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Oxyhemoglobin affinity in the armadillo
American Journal of Anatomy, 1972AbstractOxygen‐hemoglobin affinity curves determined spectrophotometrically at 26± 1° C on phosphate‐buffered hemoglobin solutions of 9 armadillos yielded a mean P50 value of 10.1 mm Hg at pH 7.4 and 18.4 mm Hg at pH 6.8. For the 18 curves determined simultaneously at both pH's the mean calculated magnitude of the Bohr effect was ϕ = − 0.43.
P L, Dementi, J D, Burke
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THE OXYHEMOGLOBIN DISSOCIATION CURVE IN ANEMIA
Annals of Internal Medicine, 1960Excerpt The primary function of hemoglobin is the transport of oxygen from the lungs to the tissues. Anemia compromises this transport mechanism and exposes the tissues to the dangers of hypoxia.
T, RODMAN, H P, CLOSE, M K, PURCELL
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Stoichiometry of the reaction of oxyhemoglobin with nitrite
Biochimica et Biophysica Acta (BBA) - Protein Structure, 1979During the reaction of oxyhemoglobin (HbO2) with nitrite, the concentration of residual nitrite, nitrate, oxygen, and methemoglobin (Hb+) was determined successively. The results obtained at various pH values indicate the following stoichiometry for the overall reaction: 4HbO2 + 4NO2- 4H+ leads to 4Hb+ + 4NO3- + O2 + 2H2 O (Hb denotes hemoglobin ...
H, Kosaka +3 more
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Measurement of percent oxyhemoglobin in the microvasculature
Journal of Applied Physiology, 1975A new method is applied to the determination of percent oxyhemoglobin (percent saturation) in whole blood. The method is based on a theoretical treatment of light absorption and scattering by particulate suspensions and requires the measurement of optical densities (D) of blood at three closely spaced wavelengths. The optical density of red blood cell
R N, Pittman, B R, Duling
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