Palindromic Sequence-Targeted (PST) PCR, Version 2: An Advanced Method for High-Throughput Targeted Gene Characterization and Transposon Display [PDF]
Frontiers in Plant Science, 2021 Genome walking (GW), a strategy for capturing previously unsequenced DNA fragments that are in proximity to a known sequence tag, is currently predominantly based on PCR.
R. Kalendar, A. Shustov, A. Schulman
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BfiI, a restriction endonuclease from Bacillus firmus S8120, which recognizes the novel non-palindromic sequence 5'-ACTGGG(N)5/4-3'. [PDF]
Nucleic Acids Research, 1998 A new type IIS restriction endonuclease Bfi I hasbeen partially purified from Bacillus firmus S8120. Bfi I recognizes the non-palindromic hexanucleotide sequence 5'-ACTGGG(N)5/4-3' and makes a staggered cut at the fifth base pair downstream of the ...
J. Vitkute +3 more
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Palindromic sequence impedes sequencing-by-ligation mechanism [PDF]
BMC Systems Biology, 2012 BackgroundCurrent next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system.
Yu-Feng Huang +4 more
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Comparison of a Repetitive Extragenic Palindromic Sequence-Based PCR Method and Clinical and Microbiological Methods for Determining Strain Sources in Cases of Nosocomial Acinetobacter baumannii Bacteremia [PDF]
Journal of Clinical Microbiology, 2002 Using a repetitive extragenic palindromic PCR (REP-PCR), we genotypically characterized strains causing nosocomial Acinetobacter baumannii infections and analyzed the source of bacteremia in 67 patients from an institution in which infections by this ...
D. Martín-Lozano +7 more
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Journal of Biological Chemistry, 2001 We isolated cDNAs encoding a novel RING finger protein (LUN), the mRNAs of which were expressed at high levels in the lung. In situ hybridization revealed that LUN mRNAs were expressed in the alveolar epithelium of the lung.
Dong Chu +7 more
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Palindromic Sequence Plays a Critical Role in Human Foamy Virus Dimerization [PDF]
Journal of Virology, 2001 The retroviral RNA genome is dimeric, consisting of two identical strands of RNA linked near their 5′ ends by a dimer linkage structure. Previously it was shown that human foamy virus (HFV) RNA transcribed in vitro contained three sites, designated SI ...
D. Cain +4 more
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Unique sequence arrangement of ribosomal genes in the palindromic rDNA molecule of Physarum polycephalum. [PDF]
Nucleic Acids Research, 1979 R-loop and restriction mapping procedures reveal the organization of coding regions at each end of the giant rDNA palindrome of Physarum polycephalum. A 19S coding region of 2.10 +/- 0.21 kb is located at each end of a very long central spacer (35.64 +/-
Gerald R. Campbell +4 more
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Typing of Clinical and Environmental Aeromonas sp. Strains by Random Amplified Polymorphic DNA PCR, Repetitive Extragenic Palindromic PCR, and Enterobacterial Repetitive Intergenic Consensus Sequence PCR [PDF]
Journal of Clinical Microbiology, 2004 A collection of 120 strains isolated from stool specimens collected from humans suffering from gastroenteritis and from environmental samples were analyzed by random amplified polymorphic DNA PCR (RAPD), repetitive extragenic palindromic PCR (REP-PCR ...
E. Szczuka, A. Kaznowski
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Bacterial repetitive extragenic palindromic sequences are DNA targets for Insertion Sequence elements [PDF]
BMC Genomics, 2006 BackgroundMobile elements are involved in genomic rearrangements and virulence acquisition, and hence, are important elements in bacterial genome evolution. The insertion of some specific Insertion Sequences had been associated with repetitive extragenic
R. Tobes, E. Pareja
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Specific binding of the diphtheria tox regulatory element DtxR to the tox operator requires divalent heavy metal ions and a 9-base-pair interrupted palindromic sequence. [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 1992 The structural gene for diphtheria toxin, tox, is carried by a family of closely related corynebacteriophages; however, the regulation of tox expression is controlled by a Corynebacterium diphtheriae-encoded regulatory element, dtxR.
X. Tao, Jessica BOYDt, J. Murphy
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