Palindromic sequence artifacts generated during next generation sequencing library preparation from historic and ancient DNA. [PDF]
Degradation-specific processes and variation in laboratory protocols can bias the DNA sequence composition from samples of ancient or historic origin.
Bastiaan Star +9 more
doaj +4 more sources
Palindromic Sequence-Targeted (PST) PCR, Version 2: An Advanced Method for High-Throughput Targeted Gene Characterization and Transposon Display [PDF]
Genome walking (GW), a strategy for capturing previously unsequenced DNA fragments that are in proximity to a known sequence tag, is currently predominantly based on PCR.
Ruslan Kalendar +4 more
doaj +2 more sources
Fluorescent Peptides Internalize HeLa Cells and Kill Multidrug-Resistant Clinical Bacterial Isolates [PDF]
Palindromic antimicrobial peptides (PAMs) constitute versatile scaffolds for the design and optimization of anticancer agents with applications in therapy, diagnosis, and/or monitoring.
Daniel Castellar-Almonacid +10 more
doaj +2 more sources
Mitochondrial genome of African rice (Oryza glaberrima): comparative analysis and phylogenetic relationships [PDF]
We report the first complete mitochondrial genome of African rice (Oryza glaberrima), spanning 376,795 bp across two circular chromosomes (GC: 43.9%) and encoding 65 genes (39 protein-coding, 21 tRNA, 5 rRNA).
Babatunde O. Kehinde +4 more
doaj +2 more sources
Palindromic sequence-targeted (PST) PCR: a rapid and efficient method for high-throughput gene characterization and genome walking. [PDF]
Kalendar R +4 more
europepmc +3 more sources
ON "VERY PALINDROMIC" SEQUENCES [PDF]
We consider the problem of characterizing the palindromic sequences 〈cd−1, cd−2, . . . , c0〉, cd−1 6= 0, having the property that for any K ∈ N there exists a number that is a palindrome simultaneously in K different bases, with 〈cd−1, cd−2, . . . , c0〉 being its digit sequence in one of those bases.
Bojan Bašić
openalex +3 more sources
Palindromic sequence impedes sequencing-by-ligation mechanism. [PDF]
Abstract Background Current next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system.
Huang YF +4 more
europepmc +4 more sources
Species-specific repetitive extragenic palindromic (REP) sequences in Pseudomonas putida [PDF]
Pseudomonas putida KT2440 is a soil bacterium that effectively colonises the roots of many plants and degrades a variety of toxic aromatic compounds. Its genome has recently been sequenced. We describe that a 35 bp sequence with the structure of an imperfect palindrome, originally found repeated three times downstream of the rpoH gene terminator, is ...
Isabel Aranda‐Olmedo
openalex +3 more sources
CRISPR genome editing using computational approaches: A survey
Clustered regularly interspaced short palindromic repeats (CRISPR)-based gene editing has been widely used in various cell types and organisms. To make genome editing with Clustered regularly interspaced short palindromic repeats far more precise and ...
Roghayyeh Alipanahi +2 more
doaj +1 more source
Efficient and specific internal cleavage of a retroviral palindromic DNA sequence by tetrameric HIV-1 integrase. [PDF]
BACKGROUND: HIV-1 integrase (IN) catalyses the retroviral integration process, removing two nucleotides from each long terminal repeat and inserting the processed viral DNA into the target DNA.
Olivier Delelis +7 more
doaj +1 more source

