Results 41 to 50 of about 2,776,901 (186)

NF2 is Essential for Human Endoderm Development

Advanced Science, EarlyView.
This study demonstrates that NF2, moesin‐ezrin‐radixin like (MERLIN) tumor suppressor (NF2) is essential for human endoderm formation. NF2 knockout human induced pluripotent stem cells fail to form endoderm both in vitro and in vivo due to yes‐associated protein 1 (YAP1) nuclear translocation, redirecting differentiation toward myofibroblast‐like cells.
Minjin Jeong, Dongjun Han, Preetida Bhetariya, D. Bradley Welling, Miodrag Stojkovic, Konstantina M. Stankovic   +5 more
wiley   +1 more source

Folate Receptor‐Mediated Delivery of Cas9 RNP for Enhanced Immune Checkpoint Disruption in Cancer Cells

Small, Volume 19, Issue 2, January 11, 2023., 2023
For therapeutic genome editing, efficient and cell‐specific transport of the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) system into target cells is desirable. Here, folic acid‐modified Cas9 ribonucleoprotein nanocarriers are presented, which mediate enhanced gene modification in folate receptor
Yi Lin, Ulrich Wilk, Jana Pöhmerer, Elisa Hörterer, Miriam Höhn, Xianjin Luo, Hongcheng Mai, Ernst Wagner, Ulrich Lächelt   +8 more
wiley   +1 more source

Assessment and Mitigation of CRISPR‐Cas9‐Induced Nontargeted Translocations

Advanced Science, EarlyView.
Various inverted repeat elements are dispersed throughout genomes. This study reveals that these elements can cause significant chromosomal rearrangements when CRISPR editing occurs in their proximity. The risk can be mitigated by incorporating inverted repeat‐homologous segments into the CRISPR system, which represents a promising strategy for ...
Zhiyang Hou, Qiyi Yi, Mengying Wu, Lijun Wu, Fanghua Li, Ting Wang, Po Bian   +6 more
wiley   +1 more source

Engineering the Immune Response to Biomaterials

Advanced Science, EarlyView.
This paper reviews immune responses to biomaterials, stages of evolution in relation to managing these responses ranging from preventing to modulating them. It also discusses state‐of‐the‐art strategies to modulate these responses and modes of their delivery.
Abolfazl Salehi Moghaddam, Mehran Bahrami, Einollah Sarikhani, Rumeysa Tutar, Yavuz Nuri Ertas, Faleh Tamimi, Ali Hedayatnia, Clotilde Jugie, Houman Savoji, Asma Talib Qureshi, Muhammad Rizwan, Chima V. Maduka, Nureddin Ashammakhi   +12 more
wiley   +1 more source

Functional analysis of a palindromic sequence required for normal replication of several staphylococcal plasmids.

Proceedings of the National Academy of Sciences of the United States of America, 1987
Most small multicopy antibiotic-resistance plasmids of Staphylococcus aureus contain a major axis of hyphenated dyad symmetry (palA) that is required for normal replication and stability, although located outside of the minimal replicon.
A. Gruss, H. F. Ross, R. Novick
semanticscholar   +1 more source

Enhancer Extrachromosomal Circular DNA ANKRD28 Elicits Drug Resistance via POU2F2‐Mediated Transcriptional Network in Multiple Myeloma

Advanced Science, EarlyView.
Enhancer eccANKRD28‐manipulated MM cells have been demonstrated to facilitate drug resistance and promote MM progression by activating the key transcription factor, POU2F2. POU2F2 interacts with sequence‐specific eccANKRD28 as well as RUNX1 and RUNX2 motifs to form the protein complex, which activates the promoter of oncogenes (IRF4, JUNB, IKZF3, et al.
Binzhen Chen, Jia Liu, Yaoxin Zhang, Changming Shi, Di Zhu, Guoqiang Zhang, Fei Xiao, Lu Zhong, Minyue Zhang, Lai Guan Ng, Honghui Huang, Tingting Lu, Jian Hou   +12 more
wiley   +1 more source

Enhanced the Trans‐Cleavage Activity of CRISPR‐Cas12a Using Metal‐Organic Frameworks as Stimulants for Efficient Electrochemical Sensing of Circulating Tumor DNA

Advanced Science, EarlyView.
This work designs manganese metal‐organic frameworks (Mn‐MOFs) co‐assembled with Cas12a/crRNA, enabling rapid release of Mn2⁺ under mild conditions. The MOFs‐induced proximity effect sustains Mn2⁺‐Cas12a/crRNA interactions, enhancing the trans‐cleavage activity of complex available to develop an electrochemical biosensor based on Mn‐MOFs‐enhanced ...
Shuai Wu, Yincheng Liu, Tianyu Zeng, Tianci Zhou, Yanting Sun, Ying Deng, Juan Zhang, Genxi Li, Yongmei Yin   +8 more
wiley   +1 more source

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

Proceedings of the National Academy of Sciences of the United States of America, 2011
Prokaryotic clustered regularly interspaced short palindromic repeat (CRISPR)/Cas (CRISPR-associated sequences) systems provide adaptive immunity against viruses when a spacer sequence of small CRISPR RNA (crRNA) matches a protospacer sequence in the ...
E. Semenova, M. M. Jore, K. Datsenko, Anna V. Semenova, E. Westra, B. Wanner, J. van der Oost, Stan J. J. Brouns, K. Severinov   +8 more
semanticscholar   +1 more source

Mechanical Load‐Induced Upregulation of Talin2 through Non‐Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis Pathogenesis

Advanced Science, EarlyView.
Facet joint osteoarthritis (FJOA), prevalent in the elderly, involves Talin2 upregulation promoting ECM degradation while suppressing synthesis. Mechanical load stabilizes Talin2 via OTUB1‐mediated non‐canonical deubiquitination, driving CCL2 expression that recruits immune cells, exacerbating inflammatory joint degeneration.
Yizhen Huang, Heng Sun, Haojie Chen, Xiangpeng Wang, Junduo Zhao, Yang Jiao, Hongyi Zhou, Haoyu Cai, Jiafeng Dai, Xuan Huang, Weiyun Chen, Jianxiong Shen   +11 more
wiley   +1 more source

Progress in COVID research and developments during pandemic

VIEW, Volume 3, Issue 6, December 2022., 2022
The pandemic respiratory disease COVID‐19 has spread over the globe within a small span of time. Generally, there are two important points are being highlighted and considered towards the successful diagnosis and treatment process. The first point includes the reduction of the rate of infections and the next one is the decrease of the death rate.
Sudheesh K. Shukla, Santanu Patra, Trupti R. Das, Dharmesh Kumar, Anshuman Mishra, Ashutosh Tiwari   +5 more
wiley   +1 more source