Results 61 to 70 of about 45,603 (283)
In Vitro Analysis of Predicted DNA-Binding Sites for the Stl Repressor of the Staphylococcus aureus SaPIBov1 Pathogenicity Island. [PDF]
PLoS ONE, 2016 The regulation model of the Staphylococcus aureus pathogenicity island SaPIbov1 transfer was recently reported. The repressor protein Stl obstructs the expression of SaPI proteins Str and Xis, latter which is responsible for mobilization initiation. Upon
Veronika Papp-Kádár +3 more
doaj +1 more source
Advanced Materials, EarlyView.An advanced‐fidelity CRISPR nuclease, FnCas9‐AF2, is rationally engineered to discriminate single‐base mismatches with unprecedented level precision. Integrated into the MUTE‐Seq workflow, FnCas9‐AF2 depletes wild‐type cell‐free DNA, thereby exposing rare tumor‐derived mutant DNA (a molecular “needle in a haystack”).
Sunghyeok Ye +20 more
wiley +1 more source
Humanized GPRC6A KGKY is a gain-of-function polymorphism in mice
Scientific Reports, 2020 GPRC6A is proposed to regulate energy metabolism in mice, but in humans a KGKY polymorphism in the third intracellular loop (ICL3) is proposed to result in intracellular retention and loss-of-function.
Min Pi +7 more
doaj +1 more source
Target motifs affecting natural immunity by a constitutive CRISPR-Cas system in Escherichia coli. [PDF]
PLoS ONE, 2012 Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated (cas) genes conform the CRISPR-Cas systems of various bacteria and archaea and produce degradation of invading nucleic acids containing sequences (protospacers) that
Cristóbal Almendros +5 more
doaj +1 more source
Bringing Attomolar Detection to the Point‐of‐Care with Nanopatterned DNA Origami Nanoantennas
Advanced Materials, EarlyView.Single DNA molecules are captured within the hotspots of DNA origami NanoAntennas arranged on a chip surface. Fluorescence is amplified, enabling direct detection on a point‐of‐care‐compatible imaging setup (visualized as bright signals against a dark background).
Renukka Yaadav +13 more
wiley +1 more source
CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning
BioTechniques, 2015 Restriction enzymes have two major limitations for cloning: they cannot cleave at any desired location in a DNA sequence and may not cleave uniquely within a DNA sequence. In contrast, the clustered regularly interspaced short palindromic repeat (CRISPR)–
Jia-Wang Wang +6 more
doaj +1 more source
A note on palindromic length of Sturmian sequences
, 2018 Frid, Puzynina and Zamboni (2013) defined the palindromic length of a finite word $w$ as the minimal number of palindromes whose concatenation is equal to $w$. For an infinite word $u$ we study $PL_{u}$, that is, the function that assigns to each positive integer $n$, the maximal palindromic length of factors of length $n$ in $u$. Recently, Frid (2018)
Ambrož, Petr, Pelantová, Edita
openaire +2 more sources
Advanced Science, EarlyView.Current preclinical studies of AAV‐mediated gene therapy explore different strategies based on the characteristics of inner ear diseases. For genetic hearing loss, approaches include the replacement of a “good gene,” removal of a “bad gene,” or direct correction of mutations through base editing.
Fan Wu +7 more
wiley +1 more source
Mobile DNA, 2017 Background Long terminal repeat retrotransposons (LTR-RTs) are major components of plant genomes. Common LTR-RTs contain the palindromic dinucleotide 5′-‘TG’–‘CA’-3′ motif at the ends.
Hao Yin +6 more
doaj +1 more source
Advanced Science, EarlyView.CMT2 mediates the maintenance of DNA methylation on the promoters of SAGs, thereby inhibiting EIN3A untimely activating SAGs’ expression. Aging‐impelled striking upregulation of EIN3A strongly suppresses the expression of CMT2 to reduce the methylation level, which releases the activation of EIN3A on SAGs, thus initiating and accelerating the ...
Dingyu Zhang +10 more
wiley +1 more source