Results 61 to 70 of about 80,959 (317)
Complete genomic DNA sequence of the East Asian spotted fever disease agent Rickettsia japonica. [PDF]
PLoS ONE, 2013 Rickettsia japonica is an obligate intracellular alphaproteobacteria that causes tick-borne Japanese spotted fever, which has spread throughout East Asia. We determined the complete genomic DNA sequence of R.
Minenosuke Matsutani +14 more
doaj +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
FEBS Open Bio, EarlyView.This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
Integrase and integration: biochemical activities of HIV-1 integrase
Retrovirology, 2008 Integration of retroviral DNA is an obligatory step of retrovirus replication because proviral DNA is the template for productive infection. Integrase, a retroviral enzyme, catalyses integration.
Deprez Eric +4 more
doaj +1 more source
Heritable and precise zebrafish genome editing using a CRISPR-Cas system. [PDF]
PLoS ONE, 2013 We have previously reported a simple and customizable CRISPR (clustered regularly interspaced short palindromic repeats) RNA-guided Cas9 nuclease (RGN) system that can be used to efficiently and robustly introduce somatic indel mutations in endogenous ...
Woong Y Hwang +8 more
doaj +1 more source
Advanced Functional Materials, EarlyView.Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley +12 more
wiley +1 more source
AlleleProfileR: A versatile tool to identify and profile sequence variants in edited genomes.
PLoS ONE, 2019 Gene editing strategies, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9), are revolutionizing biology.
Arne A N Bruyneel +3 more
doaj +1 more source
DMSO increases efficiency of genome editing at two non-coding loci. [PDF]
PLoS ONE, 2018 Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein-9 (Cas9) has become the tool of choice for genome editing.
George Stratigopoulos +4 more
doaj +1 more source
Advanced Functional Materials, EarlyView.This work presents self‐propelled CRISPR/Cas9‐functionalized Au–MRs for rapid, amplification‐free, “on‐the‐fly” DNA detection. By harnessing motion‐assisted signal recovery, the platform achieved the limit of detection in low fM DNA concentrations, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any ...
Jyoti +3 more
wiley +1 more source
On the Classification of Universal Rotor-Routers [PDF]
, 2011 The combinatorial theory of rotor-routers has connections with problems of statistical mechanics, graph theory, chaos theory, and computer science. A rotor-router network defines a deterministic walk on a digraph G in which a particle walks from a source
He, Xiaoyu
core
Palindrome complexity bounds for primitive substitution sequences
Discrete Mathematics, 2000 zbMATH Open Web Interface contents unavailable due to conflicting licenses.
Damanik, David, Zare, Douglas
openaire +1 more source